Integration of nanomaterials with nucleic acid amplification approaches for biosensing

Wang, Ziyue; Cui, Lin; Qiu, Jian-Ge; Jiang, Bing‐Hua; Zhang, Chunyang

doi:10.1016/j.trac.2020.115959

Cited by 39 publications

(27 citation statements)

References 111 publications

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“…These above-mentioned clinical demands require the NATs to be satisfied with high specificity, high sensitivity, as well as high simplicity, which, as a consequence, have driven the development of various nucleic acid detection techniques. For instance, researchers have developed signal amplification − and recognition interface engineering strategies for improving sensitivity, − used controllable hybridization approaches for improving specificity, − and used one-step design for improving simplicity (Figure b). − From the detection limit point of view, we found that the one-step-based analysis exhibited the highest detection limit in spite of its simplicity. The supplementary means including DNA nanosturcture-based regulation, nucleic acid hybridization, and amplification approaches are facilitated to improve the detection sensitivity.…”

Section: Clinical Translation-driven Natsmentioning

confidence: 93%

Nucleic Acid Tests for Clinical Translation

et al. 2021

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Nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are natural biopolymers composed of nucleotides that store, transmit, and express genetic information. Overexpressed or underexpressed as well as mutated nucleic acids have been implicated in many diseases. Therefore, nucleic acid tests (NATs) are extremely important. Inspired by intracellular DNA replication and RNA transcription, in vitro NATs have been extensively developed to improve the detection specificity, sensitivity, and simplicity. The principles of NATs can be in general classified into three categories: nucleic acid hybridization, thermal-cycle or isothermal amplification, and signal amplification. Driven by pressing needs in clinical diagnosis and prevention of infectious diseases, NATs have evolved to be a rapidly advancing field. During the past ten years, an explosive increase of research interest in both basic research and clinical translation has been witnessed. In this review, we aim to provide comprehensive coverage of the progress to analyze nucleic acids, use nucleic acids as recognition probes, construct detection devices based on nucleic acids, and utilize nucleic acids in clinical diagnosis and other important fields. We also discuss the new frontiers in the field and the challenges to be addressed.

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Section: Clinical Translation-driven Natsmentioning

confidence: 93%

Nucleic Acid Tests for Clinical Translation

et al. 2021

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“…In fluorescence detection of viruses, nucleic acids are modified with fluorescein derivatives such as ethidium bromide dye, SYBR green or TaqMan probe, which emit fluorescence under LED light sources [ 146 ]. Recently, entropy-driven amplification strategy as a novel isothermal nucleic acid amplification method has been presented for highly sensitive detection of nucleic acids.…”

Section: Recent Applications Of Paper-based Analytical Devices For Virus Detectionmentioning

confidence: 99%

Paper-based analytical devices for virus detection: Recent strategies for current and future pandemics

Özer

Henry

2021

TrAC Trends in Analytical Chemistry

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The importance of user-friendly, inexpensive, sensitive, and selective detection of viruses has been highlighted again due to the recent Coronavirus disease 2019 (COVID-19) pandemic. Among the analytical tools, paper-based devices (PADs) have become a leading alternative for point-of-care (POC) testing. In this review, we discuss the recent development strategies and applications in nucleic acid-based, antibody/antigen-based and other affinity-based PADs using optical and electrochemical detection methods for sensing viruses. In addition, advantages and drawbacks of presented PADs are identified. Current state and insights towards future perspectives are presented regarding developing POC diagnosis platform for COVID-19. This review considers state-of-the-art technologies for further development and improvement in PADs performance for virus detection.

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“…Nonetheless, detecting any of them is rather complicated since they are present in minute concentrations relative to the rest of components in those matrices. Despite being like finding a needle in haystack, the combination of novel nanomaterials with DNA-based technologies is making possible the sensitive and selective detection of ultralow concentrations of those biomarkers . These assays can be divided into three stages: (i) capture and biorecognition of the analyte on the surface of the electrode, (ii) amplification for increasing the signal, and (iii) the electrochemical sensing per se .…”

Section: Point-of-care Testing: Massive Inexpensive Fast Diagnosismentioning

confidence: 99%

Electrochemical Sensing Directions for Next-Generation Healthcare: Trends, Challenges, and Frontiers

2020

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Integration of nanomaterials with nucleic acid amplification approaches for biosensing

Cited by 39 publications

References 111 publications

Nucleic Acid Tests for Clinical Translation

Nucleic Acid Tests for Clinical Translation

Paper-based analytical devices for virus detection: Recent strategies for current and future pandemics

Electrochemical Sensing Directions for Next-Generation Healthcare: Trends, Challenges, and Frontiers

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