Integration of polycarbonate membranes in microfluidic free-flow electrophoresis

Novo, Pedro; Dell’Aica, Margherita; Jender, Matthias; Höving, Stefan; Zahedi, René P.; Janasek, Dirk

doi:10.1039/c7an01514c

Cited by 14 publications

(22 citation statements)

References 31 publications

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“…In studies where a higher sample fractionation (e.g., with 9, 11 or 24 outlets) was obtained, this could only be achieved by using tubing connections or an elaborate assembly of a tubeless interface to the main device [25]. However, it is rather difficult to integrate tubing connections and alignment into microfluidic devices in such a way that reproducible outlet flow rates can be achieved [7]. To the best of our knowledge, we present the first directly integrated chip‐to‐world interface that does not rely on a manual alignment step or tubing.…”

Section: Resultsmentioning

confidence: 99%

“…Using the same buffer conditions and dynamic coating strategy based on 0.1% (w/w) HEC, Novo et al. estimated an apparent electroosmotic mobility of 3.2 × 10 –8 m 2 /Vs [7]. The measured electroosmosis is therefore over 150–175 times smaller than in a PDMS‐based device.…”

Section: Resultsmentioning

confidence: 99%

“…First presented by Novo et al. [7,17], commercially available polycarbonate membranes meet these criteria and allow simple integration into the PDMS‐based device. In this study, a novel 3D design of a microfluidic device is presented, which allows an easy, fast, and cost‐effective production of a μFFE using high‐resolution 3D printing technology.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, complex 3D structures-as they are used for example in passive micromixers-do not need any potentially error-prone annealing steps, and manual labor is considerably reduced when compared to more traditional assembly methods [3][4][5]. Manufacturing of polydimethylsiloxane-based (PDMS) devices typically requires a master mold for each design, as well as assembly of each casting [6][7][8]. In addition to the casting methods, subtractive methods are used to fabricate microfluidic devices [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…Microfluidic free-flow electrophoresis (μFFE) is an example of a LoC device which can separate complex samples (e.g., nucleic acids [15][16][17], proteins [6,7,11,[18][19][20][21], and cells [22,23]) in a continuous manner. Usually, the electric field is applied perpendicularly to the fluidic stream, and charged compounds are deflected differently based on their specific electrophoretic mobility [24,25].…”

Section: Introductionmentioning

confidence: 99%

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Miniaturized free‐flow electrophoresis: production, optimization, and application using 3D printing technology

et al. 2020

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The increasing resolution of three‐dimensional (3D) printing offers simplified access to, and development of, microfluidic devices with complex 3D structures. Therefore, this technology is increasingly used for rapid prototyping in laboratories and industry. Microfluidic free flow electrophoresis (μFFE) is a versatile tool to separate and concentrate different samples (such as DNA, proteins, and cells) to different outlets in a time range measured in mere tens of seconds and offers great potential for use in downstream processing, for example. However, the production of μFFE devices is usually rather elaborate. Many designs are based on chemical pretreatment or manual alignment for the setup. Especially for the separation chamber of a μFFE device, this is a crucial step which should be automatized. We have developed a smart 3D design of a μFFE to pave the way for a simpler production. This study presents (1) a robust and reproducible way to build up critical parts of a μFFE device based on high‐resolution MultiJet 3D printing; (2) a simplified insertion of commercial polycarbonate membranes to segregate separation and electrode chambers; and (3) integrated, 3D‐printed wells that enable a defined sample fractionation (chip‐to‐world interface). In proof of concept experiments both a mixture of fluorescence dyes and a mixture of amino acids were successfully separated in our 3D‐printed μFFE device.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Miniaturized free‐flow electrophoresis: production, optimization, and application using 3D printing technology

et al. 2020

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Microfluidic Free‐Flow Paper Electrochromatography for Continuous Separation of Glycans

Liu

Huang

et al. 2022

ChemElectroChem

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Microfluidic free-flow electrophoresis (μ-FFE) is widely used for continuous separation of charged analytes. However, μ-FFE is limited in the separation of analytes with the same electrophoretic mobility. In this work, we developed a novel microfluidic free-flow paper electrochromatography (μ-FFPE) by combining μ-FFE with filter paper-based chromatography, enabling continuous separation of analytes through the mechanism of both μ-FFE and paper chromatography. Numerical simulation was applied to demonstrate the advantages of adding filter paper as the stationary phase of μ-FFE. With the filter paper, a more stable separation system was achieved. The device was applied to the separation of simple mixtures of dyes and complex mixtures of glycans. With the μ-FFPE fractionation, much more glycans and glycan types can be identified by LC-MS, demonstrating the value of the method in biological analysis. The μ-FFPE can provide a new platform for the separation and analysis of various biological molecules.

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Continuous flow electrophoretic separation — Recent developments and applications to biological sample analysis

Št́astná

2019

Electrophoresis

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Continuous flow electrophoretic separation with continuous sample loading provides the advantage of processing volumes of any sizes, as well as the benefit of a real‐time monitoring and optimization of the separation process. In addition, the spatial separation of the sample enables collecting multiple separated components simultaneously and in a continuous manner. The separation is usually performed in mild buffers without organic solvents and detergents (sample biological activity is retained) and it is carried out without usage of a solid support in the separation space preventing the interaction of the sample with it (high sample recovery). The method is used for the separation of proteins/peptides in proteomic applications, and its great applicability is to the separation of the cells, cellular organelles, vesicles, membrane fragments, and DNA. This review focuses on the electrophoretic separation performed in a continuous flow and it describes various electrophoretic modes and instrumental setups. Recent developments in methodology and instrumentation, the integration with other techniques, and the application to the biological sample analysis are discussed as well.

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Integration of polycarbonate membranes in microfluidic free-flow electrophoresis

Abstract: A general difficulty in the miniaturization of free-flow electrophoresis relates to the need to separate electrodes and separation bed compartments.

Cited by 14 publications

References 31 publications

Miniaturized free‐flow electrophoresis: production, optimization, and application using 3D printing technology

Miniaturized free‐flow electrophoresis: production, optimization, and application using 3D printing technology

Microfluidic Free‐Flow Paper Electrochromatography for Continuous Separation of Glycans

Continuous flow electrophoretic separation — Recent developments and applications to biological sample analysis

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