Integration of Repeatomic and Cytogenetic Data on Satellite DNA for the Genome Analysis in the Genus Salvia (Lamiaceae)

Muravenko, Olga V.; Yurkevich, Olga Yu.; Kalnyuk, Julia V.; Samatadze, Tatiana E.; Zoshchuk, Svyatoslav A.; Amosova, Аlexandra V.

doi:10.3390/plants11172244

Cited by 6 publications

(2 citation statements)

References 109 publications

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“…In the Ty1-Copia superfamily, the SIRE and Angela families were the most common, and in the Ty3-Gypsy, the Tekay chromovirus was predominant. This is typical for many species of vascular plants although the number of these retroelements can vary among taxa [44,45,62].…”

Section: Discussionmentioning

confidence: 99%

“…SatDNAs are often associated with heterochromatin and are localized in certain regions of chromosomes, which makes it possible to identify chromosome pairs in a karyotype, detect different chromosome rearrangements, estimate the range of chromosome variability and clarify species relationships [44,45,62,66,72]. The analysis of the chromosome distribution of the oligonucleotide satDNAs in the karyotypes of C. officinalis, C. stellata, C. tripterocarpa, and C. arvensis allowed us to detect three satDNAs (Cal 2, Cal 43, and Cal 163), which presented species-specific localization on the chromosomes of all the studied species and might be effective markers for the analysis of karyotypes within Calendula.…”

Section: Discussionmentioning

confidence: 99%

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Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers

Samatadze,

Yurkevich,

Khazieva

et al. 2023

Plants

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The taxonomically challenging genus Calendula L. (Asteraceae) includes lots of medicinal species characterized by their high morphological and karyological variability. For the first time, a repeatome analysis of a valuable medicinal plant Calendula officinalis L. was carried out using high-throughput genome DNA sequencing and RepeatExplorer/TAREAN pipelines. The FISH-based visualization of the 45S rDNA, 5S rDNA, and satellite DNAs of C. officinalis was performed on the chromosomes of C. officinalis, C. stellata Cav., C. tripterocarpa Rupr., and C. arvensis L. Three satellite DNAs were demonstrated to be new molecular chromosome markers to study the karyotype structure. Karyograms of the studied species were constructed, their ploidy status was specified, and their relationships were clarified. Our results showed that the C. officinalis karyotype differed from the karyotypes of the other three species, indicating its separate position in the Calendula phylogeny. However, the presence of common repeats revealed in the genomes of all the studied species could be related to their common origin. Our findings demonstrated that C. stellata contributed its genome to allotetraploid C. tripterocarpa, and C. arvensis is an allohexaploid hybrid between C. stellata and C. tripterocarpa. At the same time, further karyotype studies of various Calendula species are required to clarify the pathways of chromosomal reorganization that occurred during speciation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers

Samatadze,

Yurkevich,

Khazieva

et al. 2023

Plants

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First insight into the genomes of the Pulmonaria officinalis group (Boraginaceae) provided by repeatome analysis and comparative karyotyping

Kobrlová,

Čížková,

Zoulová

et al. 2024

BMC Plant Biol

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Intraspecific variability of Salvia officinalis (Lamiaceae) according to DNA repeats chromosome patterns

Muravenko,

Kalnyuk,

Yurkevich

et al. 2023

bpssm

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Salvia officinalis L. (Lamiaceae) is a widespread economically valuable species. Due to the high content of essential oil, it is actively used in pharmaceutical, food and perfume industries. However, the chromosome structure and organization of its genome is still not well understood. For the first time, an analysis of intraspecific variability of S. officinalis was carried out based on the patterns of FISH localization of various DNA repeats in karyotypes of four species samples and two varieties from different habitats. Patterns of chromosome localization on of classical markers, 45S rDNA and 5S rDNA, confirmed the stability of genomes of the studied species samples and varieties. However, the intraspecific variability in localization of SO202 in karyotypes of the studied samples was found. In addition to the main set of chromosomes, B chromosomes were found in some karyotypes, including those containing 45S rDNA and SO202 clusters. The results of this study allowed us to identify all chromosome pairs in karyotypes and construct karyograms of the studied samples of S. officinalis. Our findings confirmed the stability of karyotypes of these samples according to the classical chromosomal markers but also demonstrated the presence of intraspecific variability in localization of the SO202 repeat, which can be used in breeding new varieties of S. officinalis.

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Integration of Repeatomic and Cytogenetic Data on Satellite DNA for the Genome Analysis in the Genus Salvia (Lamiaceae)

Cited by 6 publications

References 109 publications

Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers

Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers

First insight into the genomes of the Pulmonaria officinalis group (Boraginaceae) provided by repeatome analysis and comparative karyotyping

Intraspecific variability of Salvia officinalis (Lamiaceae) according to DNA repeats chromosome patterns

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