Integration of sperm sexing technology into the ART toolbox

Maxwell, W.M.C.; Evans, G.; Hollinshead, Fiona K.; Bathgate, R.; Graaf, S.P. de; Eriksson, Bengt; Gillan, L.; Morton, Katherine; O’Brien, JK

doi:10.1016/j.anireprosci.2004.04.013

Cited by 129 publications

(41 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even if the cost of each straw of sexed sperm is doubled compared to unsexed straw, it would still be profitable due to the increased production of replacement dairy heifers, or steers [4–6]. After invention of sperm sorting, sexed sperm have been used for artificial insemination (AI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a number of species [7–9]. A reduced efficiency in the production of embryos in vitro is usually reported though, when using sexed spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the current limitations, constraints, and inefficiencies of in vitro embryo production procedures, it is likely that a synergistic positive effect can be achieved by utilizing sperm sexing technology. In fact, IVF requires a much lower number of spermatozoa than does AI, in order to achieve a reasonable number of embryos produced in vitro [7]. Moreover, a higher efficiency will ultimately be reached because producing offspring of the desired sex will be cost effective, nearly half cost of those animals produced with unsexed sperm.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oocyte Source and Hormonal Stimulation forIn VitroFertilization Using Sexed Spermatozoa in Cattle

Presicce

Gong

et al. 2011

Veterinary Medicine International

View full text Add to dashboard Cite

The aim of this study was to investigate the efficiency of in vitro embryo production in cattle utilizing sexed sperm from two bulls and oocytes recovered by OPU. Twenty donor animals were employed in eight OPU replicates: the first four OPU trials were conducted on animals without hormone treatment, and the last four were run on the same animals, following FSH subcutaneous and intramuscular administration. A higher rate of blastocyst development was recorded in stimulated, as compared to nonstimulated animals, (25.2% versus 12.8%, P = .001). Ocytes derived from slaughterhouse (SH) ovaries were also fertilized with sperm from the same bulls. Overall, non-sexed sperm used with oocytes derived from SH ovaries was significantly more efficient for blastocyst development than was sexed sperm with these same SH derived oocytes and sexed sperm with stimulated donor oocytes (39.8% versus 25.0% and 25.2%, P = .001). In conclusion, the use of sexed sperm with OPU-derived oocytes resulted in a significantly higher blastocyst development when donors were hormonally stimulated; furthermore, the level of efficiency achieved was comparable to that attained when the same sexed sperm was tested on oocytes derived from SH ovaries.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oocyte Source and Hormonal Stimulation forIn VitroFertilization Using Sexed Spermatozoa in Cattle

Presicce

Gong

et al. 2011

Veterinary Medicine International

View full text Add to dashboard Cite

show abstract

“…This technique exploits differences in DNA content (Johnson et al 1989) between the X and Y chromosomes, which can be detected by fluorescenceactivated cell sorting after staining with a DNA binding dye (Hoechst 33342, a bisbenzimide-based membrane permeable probe). The technique has now been successfully applied to a wide range of species, produced innumerable live offspring after artificial insemination, and has been the subject of many reviews (Maxwell et al 2004, Garner 2006, Bathgate 2008, Rath et al 2009b). Despite its considerable advantages, industry adoption has been hampered by the additional expense and reduced capabilities of sex-sorted spermatozoa compared with their non-sorted counterparts (Rath et al 2009b).…”

Section: Sex-sortingmentioning

confidence: 99%

Sperm surface changes and physiological consequences induced by sperm handling and storage

2011

View full text Add to dashboard Cite

Spermatozoa interact with their immediate environment and this contact remodels the sperm surface in preparation for fertilisation. These fundamental membrane changes will be critically covered in this review with special emphasis on the very specific surface destabilisation event, capacitation. This process involves very subtle and intricate modifications of the sperm membrane including removal of suppression (decapacitation) factors and changes in the lateral organisation of the proteins and lipids of the sperm surface. Processing of sperm for assisted reproduction (storage, sex-sorting, etc.) subjects spermatozoa to numerous stressors, and it is possible that this processing overrides such delicate processes resulting in sperm instability and cell damage. To improve sperm quality, novel mechanisms must be used to stabilise the sperm surface during handling. In this review, different types of membrane stress are considered, as well as novel surface manipulation methods to improve sperm stability.

show abstract

“…Since the first report by Johnson et al (1989) in rabbits, flowcytometric sexing technology has been shown to be efficacious in several species including buffalo ( Johnson, 2000;Seidel Jr and Garner, 2002;Maxwell et al, 2004;Lu et al, 2007;Liang et al, 2008). The earlier studies failed to notice difference in the DNA content between X-and Y-bearing sperm because of random orientation of the sperm in the flow-cytometer fluid stream ( Johnson and Welch, 1999).…”

Section: Flow-cytometric Sorting Of Semenmentioning

confidence: 98%