Integration of systematic review, lipidomics with experiment verification reveals abnormal sphingolipids facilitate diabetic retinopathy by inducing oxidative stress on RMECs

Yuan, Zhenshuang; Tian, Yue; Zhang, Cong; Wang, Mingshuang; Xie, Jiaqi; Wang, Can; Huang, Jianmei

doi:10.1016/j.bbalip.2023.159382

Cited by 2 publications

(1 citation statement)

References 76 publications

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“…6B). We studied the relationship between nodes and edges by referring to previous research methods of Zhenshuang Yuan (Zhenshuang et al, 2023). Using Cytoscape, we performed further analysis of the imported PPI network for more information.…”

Section: Bioinformatics Analysis Of the Anti-crc Mechanism Of C2fmentioning

confidence: 99%

Ethyl 2,2-difluoro-2-(2-oxo-2H-chromen-3-yl) acetate inhibits the malignant biological behaviors of colorectal cancer by restricting the phosphorylation and nuclear translocation of STAT3

LIN,

LIU,

et al. 2024

Preprint

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To investigate the effect of a novel coumarin derivative, ethyl 2,2-difluoro-2 - (2-oxo-2H-chromen-3-yl) acetate (C2F), on the malignant biological behaviors of colorectal cancer (CRC) and elucidate its mechanism. In vitro, the effects of C2F on the proliferation, apoptosis, migration, invasion, and cell cycle of CRC cells were analyzed by MTT assay, EdU stainning, colony formation assay, flow cytometry, wound healing and transwell assay. The anti-CRC activity of C2F was evaluated in a nude mice xenograft model in vivo. Western blot was conducted to detect the expression of protein in cells and mice tissue. Then, the potential targets of C2F in CRC were predicted by network pharmacology analysis and molecular docking. The localization of STAT3 was observed through immunofluorescence experiment. C2F inhibits CRC cell proliferation, promotes CRC cell apoptosis, hinders CRC cell migration and invasion, and prevents the cell cycle from entering the G2/M phase. In vivo, C2F inhibited tumor growth in xenograft model. C2F inhibited signal transduction and activator of transcription 3 (STAT3) phosphorylation and blocked interleukin-6 (IL-6)-induced STAT3 nuclear translocation. C2F inhibits the malignant biological behavior of CRC by limiting STAT3 phosphorylation and entry into the nucleus.

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Section: Bioinformatics Analysis Of the Anti-crc Mechanism Of C2fmentioning

confidence: 99%

Ethyl 2,2-difluoro-2-(2-oxo-2H-chromen-3-yl) acetate inhibits the malignant biological behaviors of colorectal cancer by restricting the phosphorylation and nuclear translocation of STAT3

LIN,

LIU,

et al. 2024

Preprint

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Potential testicular aging biomarkers identified in seminal plasma byproteomic analysis

Libo Xiong,

Xi Wang,

Min Nie

et al. 2024

IJKD

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Introduction. This research aims to identify biomarkers for the early detection of testicular aging by comparing protein expression levels in seminal plasma from young and older individuals.Methods. Seminal plasma samples from five young and five older men were analyzed using Data Independent Acquisition (DIA) proteomics to identify proteins with differential expression. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were applied to these proteins. Further validation of selected proteins was conducted in new samples of seminal plasma and serum using enzyme-linked immunosorbent assay (ELISA).Results. Using the DIA method, 73 proteins with altered expression levels were detected in seminal plasma-46 exhibited increased expression, while 27 showed decreased expression.GO and KEGG analyses linked these proteins to lipid metabolism, inflammation, and cellular aging processes. ELISA tests confirmed that the proteins GNA13, SAA4, and CYCS were prominently expressed in older seminal plasma samples. Serum analysis using ELISA indicated consistent levels of SAA4 and CYCS compared to seminal plasma but revealed a delayed expression of GNA13.Conclusion. The elevated levels of GNA13 in seminal plasma, which are observed before its detection in serum, correlate with the aging process. This indicates that GNA13 could serve as a promising early indicator for diagnosing testicular aging.

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Integration of systematic review, lipidomics with experiment verification reveals abnormal sphingolipids facilitate diabetic retinopathy by inducing oxidative stress on RMECs

Cited by 2 publications

References 76 publications

Ethyl 2,2-difluoro-2-(2-oxo-2H-chromen-3-yl) acetate inhibits the malignant biological behaviors of colorectal cancer by restricting the phosphorylation and nuclear translocation of STAT3

Ethyl 2,2-difluoro-2-(2-oxo-2H-chromen-3-yl) acetate inhibits the malignant biological behaviors of colorectal cancer by restricting the phosphorylation and nuclear translocation of STAT3

Potential testicular aging biomarkers identified in seminal plasma byproteomic analysis

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