Integration of Technology Into Clinical Practice

Doern, Christopher D.

doi:10.1016/j.cll.2013.03.004

Cited by 7 publications

(5 citation statements)

References 74 publications

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“…The critical step that has the most influence on the quality of the mass spectra is sample preparation [29,[31][32][33]. Several studies have shown that the type of medium from which an organism is selected for MALDI-TOF identification can impact the quality of the result generated [34]. Also, the amount of bacteria necessary to obtain a useful protein profile for identification has influence on the quality of the obtained spectrum [35].…”

Section: Discussionmentioning

confidence: 99%

Use of MALDI-TOF mass spectrometry fingerprinting to characterize Enterococcus spp. and Escherichia coli isolates

Santos

Capelo

Santos

et al. 2015

Journal of Proteomics

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Section: Discussionmentioning

confidence: 99%

Use of MALDI-TOF mass spectrometry fingerprinting to characterize Enterococcus spp. and Escherichia coli isolates

Santos

Capelo

Santos

et al. 2015

Journal of Proteomics

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“…Though not uniformly ready for routine clinical use, rapid diagnostics have the potential to dramatically alter the field of antimicrobial stewardship [56]. Rapid diagnostic tools have the potential to identify bacterial, fungal, and viral causes of sepsis at a much earlier stage than traditional culture methods, which should reduce time to appropriate therapy, a known determinant of sepsis mortality [4, 5, 57].…”

Section: Antibiotic Stewardshipmentioning

confidence: 99%

The intensive care medicine research agenda on multidrug-resistant bacteria, antibiotics, and stewardship

et al. 2017

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Purpose: To concisely describe the current standards of care, major recent advances, common beliefs that have been contradicted by recent trials, areas of uncertainty, and clinical studies that need to be performed over the next decade and their expected outcomes with regard to the management of multidrug-resistant (MDR) bacteria, antibiotic use, and antimicrobial stewardship in the intensive care unit (ICU) setting. Methods: Narrative review based on a systematic analysis of the medical literature, national and international guidelines, and expert opinion. Results: The prevalence of infection of critically ill patients by MDR bacteria is rapidly evolving. Clinical studies aimed at improving understanding of the changing patterns of these infections in ICUs are urgently needed. Ideal antibiotic utilization is another area of uncertainty requiring additional investigations aimed at better understanding of dose optimization, duration of therapy, use of combination treatment, aerosolized antibiotics, and the integration of rapid diagnostics as a guide for treatment. Moreover, there is an imperative need to develop non-antibiotic approaches for the prevention and treatment of MDR infections in the ICU. Finally, clinical research aimed at demonstrating the beneficial impact of antimicrobial stewardship in the ICU setting is essential. Conclusions: These and other fundamental questions need to be addressed over the next decade in order to better understand how to prevent, diagnose, and treat MDR bacterial infections. Clinical studies described in this research agenda provide a template and set priorities for investigations that should be performed in this field.

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“…The mass-to-charge ratio of ions is proportional, and the mass-to-charge ratio can be measured according to the flight time to the detector, and a characteristic fingerprint can be obtained through software processing [54]. MALDI-TOF MS technology has the characteristics of rapidity, accuracy, sensitivity and automation [55], and gradually occupies an important position in the identification of microbiology laboratories [56]. Rychert et al [57] conducted a multicenter study on Gram-positive aerobic bacteria, and the results showed that in 1146 Gram-positive bacteria samples, the accuracy rate at the species level was 92.8%, and the accuracy at the genus level could reach 95.5%.…”

Section: Mass Spectrometrymentioning

confidence: 99%

Recent Progress in the Diagnosis of Staphylococcus in Clinical Settings

Zhang¹,

Gu²,

Usman³

et al. 2023

Infectious Diseases

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Staphylococci are mainly found on the skin or in the nose. These bacteria are typically friendly, causing no harm to healthy individuals or resulting in only minor issues that can go away on their own. However, under certain circumstances, staphylococcal bacteria could invade the bloodstream, affect the entire body, and lead to life-threatening problems like septic shock. In addition, antibiotic-resistant Staphylococcus is another issue because of its difficulty in the treatment of infections, such as the notorious methicillin-resistant Staphylococcus aureus (MRSA) which is resistant to most of the currently known antibiotics. Therefore, rapid and accurate diagnosis of Staphylococcus and characterization of the antibiotic resistance profiles are essential in clinical settings for efficient prevention, control, and treatment of the bacteria. This chapter highlights recent advances in the diagnosis of Staphylococci in clinical settings with a focus on the advanced technique of surface-enhanced Raman spectroscopy (SERS), which will provide a framework for the real-world applications of novel diagnostic techniques in medical laboratories via bench-top instruments and at the bedside through point-of-care devices.

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Integration of Technology Into Clinical Practice

Cited by 7 publications

References 74 publications

Use of MALDI-TOF mass spectrometry fingerprinting to characterize Enterococcus spp. and Escherichia coli isolates

Use of MALDI-TOF mass spectrometry fingerprinting to characterize Enterococcus spp. and Escherichia coli isolates

The intensive care medicine research agenda on multidrug-resistant bacteria, antibiotics, and stewardship

Recent Progress in the Diagnosis of Staphylococcus in Clinical Settings

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