Integration of the full-length HPV16 genome in cervical cancer and Caski and Siha cell lines and the possible ways of HPV integration

Xu, Feng; Cao, Meng; Qinfeng, Shi; Chen, Hongwei; Wang, Yili; Li, Xu

doi:10.1007/s11262-014-1164-7

Cited by 34 publications

(23 citation statements)

References 20 publications

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“…These findings suggest a lower risk of persistence and progression in women with ASCUS Pap smears that were infected with the EUR-350T isolate [8,15,20]. However our technique risks to under-quantify the percentage of integration if the gene E2 is intact after integration, for example in case of tandem-repeated HPV integrated genomes induce by the rolling circle replication mechanism [56,57]. But, HR-HPV DNA is frequently integrated into the chromosome of cervical cancer cells and is occasionally found amplified as tandem-repeated HPV integrated genomes, as typically observed in CaSki cells [21,40].…”

Section: Discussionmentioning

confidence: 96%

Relationship between E6-E7 lineage sequences, viral loads, and integration of HPV16 in women with atypical squamous cells of undetermined significance (ASCUS) pap smears

Loubatier¹,

Monte²,

Cannavo³

et al. 2018

Obstet Gynecol Rep

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HPV16 DNA associated with histologic high-grade precancer lesions (cervical intraepithelial neoplasia grade 2 or 3 [CIN2 or CIN3]) is sometimes found in women with atypical squamous cells of undetermined significance (ASCUS) Pap smears, the mildest form of cellular abnormality in a cervical smear. This study evaluated, in a population of patients with ASCUS Pap smears, the prevalence of different HPV16 lineages (or isolates) and the physical status, total viral load, and integrated viral load of HPV16 DNA in a population, stratified by HPV16 isolates (primarily either EUR-350T or EUR-350G) that was found. We demonstrated, in smears of women diagnosed with ASCUS and infected with EUR-350G HPV16 isolates (sublineage A1) that both the physical status of HPV16 and the distribution of integrated HPV16 DNA viral loads were different when compared to a population infected with EUR-350T HPV16 isolates. We showed that the mean and median percentages of HPV16 DNA were higher, and a distribution of log 10 integrated HPV16 viral load was more homogeneous in a population infected with EUR-350G HPV16 isolates than in a population infected with EUR-350T HPV16 isolates. No difference was found in terms of total HPV16 viral load between these two isolates. Here, we investigate E6-E7 sequencing as an easy technique to detect women with a greater proportion of integrated HPV genome, and we suggest an adaptation of the current protocol for monitoring women with ASCUS Pap smears.

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Section: Discussionmentioning

confidence: 96%

Relationship between E6-E7 lineage sequences, viral loads, and integration of HPV16 in women with atypical squamous cells of undetermined significance (ASCUS) pap smears

Loubatier¹,

Monte²,

Cannavo³

et al. 2018

Obstet Gynecol Rep

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“…The fluorescence intensity was regularly read with the qPCR machine at 30 s intervals. HeLa cells were used for HPV18 positive cervical carcinoma cells (19), SiHa cells for HPV16 positive cervical carcinoma cells (20), and C-33A cells for HPV-negative cervical carcinoma cell (21). …”

Section: Methodsmentioning

confidence: 99%

Detection of Nucleic Acids with a Novel Stem-Loop Primer Rolling Circle Amplification Technique

Zhang

Wang

et al. 2018

BioTechniques

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This paper presents a new rolling circle amplification (RCA) technique using stem-loop primers (SLP). The technique enables detection of target DNA by either linear or exponential amplification (SLP-lRCA and SLP-eRCA) in both liquid and solid phases. For solid-phase detection, SLP-eRCA detects nucleic acids in four steps: (1) covalently immobilize an array of capture probes (CP) on a solid support; (2) hybridize the CP array with the DNA sample; (3) incubate the CP array with an RCA reaction containing two SLPs; (4) image the CP array. SLP-eRCA detects nucleic acids in liquid phase in one step: a real-time RCA reaction containing the DNA sample and two SLPs. Both liquid- and solid-phase detection methods employ a general rolling circle and an SLP. The other SLP is specific to the target. The technique was verified by detecting synthesized oligonucleotides and six different human papillomaviruses (HPVs), both in liquid phase and on a solid surface. The technique also detected two high-risk HPVs (HPV16 and HPV18) in cervical carcinoma cells (HeLa and SiHa) and clinical samples. This study provides proof-of-concept for the new RCA technique for nucleic acid detection, which overcomes major limitations of current RCA approaches.

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“…Moreover, chronic high-risk HPV infection and the integration of elements of the viral genome into the human chromosome have proven to be pathogenic factors for cervical malignant transformation and lesions (12,13).…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, few reports (11)(12)(13)(14) have described the HPV integration site. The objective of the present study was to assess HPV18 infection and its location in the human genome in the setting of EC.…”

Section: Introductionmentioning

confidence: 99%

Integration sites of human papillomavirus 18 in esophageal cancer samples

Yan

Sun

et al. 2018

Oncol Lett

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Abstract. To investigate the association between human papillomavirus (HPV) infection and esophageal cancer, genomic DNA was isolated from 189 samples obtained from patients with esophageal carcinoma, and HPV DNA was identified using the polymerase chain reaction (PCR) with the following specific primers: My09/11 for HPV L1 and HPV18 E6 for HPV18. The HPV18 gene products were sequenced to identify the HPV genotype and the HPV18 integration site was verified using PCR amplification of papillomavirus oncogene transcripts. HPV18 oncogene transcript products were ligated into a pMD-18T plasmid vector and sequenced to confirm the physical location of HPV18 integration. Of the 189 samples, 168 were positive for HPV, of which 33 were positive for HPV18. The sequencing analysis identified two HPV18 E6-positive samples containing one mutation and two samples containing two mutations in the viral DNA. In total ~600 bp of the HPV18 oncogene transcript was detected in three esophageal cancer samples. Sequence analysis revealed that, in two patients, the HPV18 infection was integrated into human chromosome 5, whereas in the remaining sample the virus was integrated into human chromosome 2. The high prevalence of HPV18 infection suggested that HPV18 infection is a pathogenic factor in esophageal carcinoma progression. The integration of HPV18 DNA into the host cell genome suggests that persistent HPV infection has a role in esophageal epithelial cell malignant transformation

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Integration of the full-length HPV16 genome in cervical cancer and Caski and Siha cell lines and the possible ways of HPV integration

Cited by 34 publications

References 20 publications

Relationship between E6-E7 lineage sequences, viral loads, and integration of HPV16 in women with atypical squamous cells of undetermined significance (ASCUS) pap smears

Relationship between E6-E7 lineage sequences, viral loads, and integration of HPV16 in women with atypical squamous cells of undetermined significance (ASCUS) pap smears

Detection of Nucleic Acids with a Novel Stem-Loop Primer Rolling Circle Amplification Technique

Integration sites of human papillomavirus 18 in esophageal cancer samples

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