Integration of tri-polar microelectrodes for performance enhancement of an impedance biosensor

Sherif, Sameh; Morsy, Omar E.; Ziko, Laila; Siam, Rania; Ghallab, Yehya H.; Ismail, Yehea

doi:10.1016/j.sbsr.2020.100329

Cited by 11 publications

(12 citation statements)

References 13 publications

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“…µEIS has also been utilized to investigate the electrical properties of tissues such as brain tissue and to develop biosensors for detecting specific molecules in biological samples. The passive electrical properties and dielectric characteristics [ 21 ], such as the impedance of biological cells, including human cancer cells, can be elucidated using µEIS, which can describe the fluctuation in cell impedance across a range of frequencies [ 25 ]. These fluctuations are due to the permittivity and conductivity contributions of various polarization processes that occur upon changes in the externally applied field [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…These fluctuations are due to the permittivity and conductivity contributions of various polarization processes that occur upon changes in the externally applied field [ 26 ]. Thus, defining the parameters, such as applied voltage and frequency, are essential for analysis to improve the system’s reliability in extracting cell dielectric features [ 21 , 27 , 28 ], including cancer, stem, and neural cells [ 25 ].µEIS has been used to study the properties of tissues and organelles, including changes in their composition or structure [ 29 ]. Researchers [ 30 ] also performed an impedance analysis on human cervical cancer cell lines as a function of frequency.…”

Section: Introductionmentioning

confidence: 99%

“…At low frequencies, the cell membrane acts as an insulator, while at high frequencies, it behaves as a conductor. In other words, the measured electrical impedance at low frequencies only reflects the impedance of the extracellular fluid since the electric field passes around the cell membrane [ 25 ]. In contrast, at high frequencies, the cell membrane impedance is so tiny that it cannot block the incident electric field, causing the cell to become permeable [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

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Optimization design of interdigitated microelectrodes with an insulation layer on the connection tracks to enhance efficiency of assessment of the cell viability

et al. 2023

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Background Microelectrical Impedance Spectroscopy (µEIS) is a tiny device that utilizes fluid as a working medium in combination with biological cells to extract various electrical parameters. Dielectric parameters of biological cells are essential parameters that can be extracted using µEIS. µEIS has many advantages, such as portability, disposable sensors, and high-precision results. Results The paper compares different configurations of interdigitated microelectrodes with and without a passivation layer on the cell contact tracks. The influence of the number of electrodes on the enhancement of the extracted impedance for different types of cells was provided and discussed. Different types of cells are experimentally tested, such as viable and non-viable MCF7, along with different buffer solutions. This study confirms the importance of µEIS for in vivo and in vitro applications. An essential application of µEIS is to differentiate between the cells’ sizes based on the measured capacitance, which is indirectly related to the cells’ size. The extracted statistical values reveal the capability and sensitivity of the system to distinguish between two clusters of cells based on viability and size. Conclusion A completely portable and easy-to-use system, including different sensor configurations, was designed, fabricated, and experimentally tested. The system was used to extract the dielectric parameters of the Microbeads and MCF7 cells immersed in different buffer solutions. The high sensitivity of the readout circuit, which enables it to extract the difference between the viable and non-viable cells, was provided and discussed. The proposed system can extract and differentiate between different types of cells based on cells’ sizes; two other polystyrene microbeads with different sizes are tested. Contamination that may happen was avoided using a Microfluidic chamber. The study shows a good match between the experiment and simulation results. The study also shows the optimum number of interdigitated electrodes that can be used to extract the variation in the dielectric parameters of the cells without leakage current or parasitic capacitance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimization design of interdigitated microelectrodes with an insulation layer on the connection tracks to enhance efficiency of assessment of the cell viability

et al. 2023

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“…According to the authors' best knowledge, the proposed sensing method is the world's first design for tissue resistivity measurement and interpretation based on a tripolar configuration and existing RAMIS instruments. Unlike other existing technologies [28], the proposed method utilizes flexible separate electrodes, and is based on coordinated control for measurement and data analysis.…”

Section: Introductionmentioning

confidence: 99%

An Electrical Bioimpedance Scanning System for Subsurface Tissue Detection in Robot Assisted Minimally Invasive Surgery

Cheng

Schwaner

Dall’Alba

et al. 2022

IEEE Trans. Biomed. Eng.

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In Robot Assisted Minimally Invasive Surgery, discriminating critical subsurface structures is essential to make the surgical procedure safer and more efficient. In this paper, a novel robot assisted electrical bio-impedance scanning (RAEIS) system is developed and validated using a series of experiments. The proposed system constructs a tri-polar sensing configuration for tissue homogeneity inspection. Specifically, two robotic forceps are used as electrodes for applying electric current and measuring reciprocal voltages relative to a ground electrode which is placed distal from the measuring site. Compared to existing electrical bioimpedance sensing technology, the proposed system is able to use miniaturized electrodes to measure a site flexibly with enhanced subsurfacial detection capability. This paper presents the concept, the modeling of the sensing method, the hardware design, and the system calibration. Subsequently, a series of experiments are conducted for system evaluation including finite element simulation, saline solution bath experiments and experiments based on ex vivo animal tissues. The experimental results demonstrate that the proposed system can measure the resistivity of the material with high accuracy, and detect a subsurface non-homogeneous object with 100% success rate. The proposed parameters estimation algorithm is able to approximate the resistivity and the depth of the subsurface object effectively with one fast scanning.

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“…Source: Adapted from BUSCAGLIA et al 35 An alternative to generate variable signal is a system known as direct digital synthesizer (DDS), also widely employed on instruments for impedance spectroscopy. 16,32,[48][49][50] given by f OUT = f OSC (FCW / 2 N ) . Most ICs used in impedance spectroscopy use DDSs rather than PLLs, which require complex and numerous circuits for frequency sweeping.…”

Section: Frequency Generationmentioning

confidence: 99%

Development of a Portable Impedance Spectrometer

Buscaglia¹

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 Mamá, for being unconditionally present, for helping me with the software development for so many days, and most important, for being my mother. Sisters, grandparents (Sílvia and Vivaldo, you too!), uncles and aunts, cousins, Mariana and Marcelo, for accompanying me during these years. Friends from São Carlos, Bariloche and abroad, all essential for me to work every day. Bianca, for the deep friendship, for the teachings, and especially for the months of quarantine together, which were so important for this project to succeed. Larissa, for being my partner and having participated with much love in my daily life during this adventure. Professor João P. Carmo, for your guidance with the electronics and for your collaboration during the writing of the review article and patent application.  Tía Mariana, for your thoughtful collaboration on designing the logo and the casing of Simple-Z.  Gounella, Poste, Tiago and Melk, for all your time helping me in the fabrication of Simple-Z.  Paulo, for your time and dedication on helping me with the sodium sulfate, among others, experiments.  Tío Car, for your very careful mentoring during the experiments with cyanobacteria. Fellipe, for the many hours spent in the laboratory helping me on the experiments with cyanobacteria and for helping me write the correspondent part in this dissertation. Andrey and Juliana, for working with me over this years and especially on the article on SARS-CoV-2. Professor Margaret, even though the pandemic brought down our plans, for your willingness and efforts on receiving me in Ireland for working together. Willyan, Priscila and all Biosens, for supporting my personal development. All the co-authors of the works published throughout my Masters, it was a pleasure working with you.

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Integration of tri-polar microelectrodes for performance enhancement of an impedance biosensor

Cited by 11 publications

References 13 publications

Optimization design of interdigitated microelectrodes with an insulation layer on the connection tracks to enhance efficiency of assessment of the cell viability

Optimization design of interdigitated microelectrodes with an insulation layer on the connection tracks to enhance efficiency of assessment of the cell viability

An Electrical Bioimpedance Scanning System for Subsurface Tissue Detection in Robot Assisted Minimally Invasive Surgery

Development of a Portable Impedance Spectrometer

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