Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in Cordyceps chanhua under H2O2-Induced Oxidative Stress

Abstract

Cordyceps chanhua is an important cordycipitoid mushroom widely used in Asia and beyond. Beauvericin (BEA), one of the bioactive compounds of C. chanhua, has attracted much attention because of its medicinal value and food safety risk. In order to clear up the relationship between oxidative stress and BEA synthesis, we investigated the impact of H2O2-induced oxidative stress on the secondary metabolism of C. chanhua using untargeted metabolomics and a transcript profiling approach. Metabolic profiling of C. chanhua mycelia found that in total, 73 differential metabolites were identified, including organic acids, phospholipids, and non-ribosomal peptides (NRPs), especially the content of BEA, increasing 13-fold under oxidative stress treatment. Combining transcriptomic and metabolomic analyses, we found that the genes and metabolites associated with the NRP metabolism, especially the BEA biosynthesis, were highly significantly enriched under H2O2-induced stress, which indicated that the BEA metabolism might be positive in the resistance of C. chanhua to oxidative stress. These results not only aid in better understanding of the resistance mechanisms of C. chanhua against oxidative stress but also might be helpful for molecular breeding of C. chanhua with low BEA content.

Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of Sanghuangporus baumii

“…Transcriptomescan provide full coverage of transcript abundance expression [ 28 ]. We excavated 57 genes involved in terpenoid synthesis and some high-transcript genes based on transcriptomes [ 13 , 29 ], which were annotated to the MVA pathway ( Table S4 ).…”

“…Metabolome and transcriptome analyses have become common technical means to explore the underlying molecular mechanisms of fungal metabolic biosynthesis [ 28 , 30 ], and their combination can provide an in-depth and comprehensive understanding of the relationship between gene function and metabolites and/or phenotypes [ 5 , 33 ]. In this study, we observed a correlation and linkage between terpenoid products and terpenoid synthase gene expression levels at different developmental stages ( Figure 3 D and Figure 5 ).…”

Genomic Comparison of Two Species of Samsoniella with Other Genera in the Family Cordycipitaceae

“…More than 260 healthcare products had been developed with S. hepiali as a raw material, with Jinshuibao capsules having the highest output value [2]. Cordycipitaceae species have produced a variety of metabolites, such as bassianolide [4], fumosorinone [5], ergosterol [6], 8-O-methylindigotide B [7], cordycerebroside B [8], cepharosporolides C [9], beauvericin [10], beauveriolide [11], oosporein [12], bassiatin [13], ophiocordin [14], cordycepin [15], oosporein [16] and cordycepic acid [17]. It can be seen that there is still a lot of room for exploration of the potential of Cordycipitaceae species to produce compounds.…”

Genomic Comparative Analysis of Cordyceps pseudotenuipes with Other Species from Cordyceps

“…[1][2][3]. Genus Cordyceps had many bioactive components, including cordycepin [4], cordyceps polysaccharides [5], alkaloids [6], cordycepic acids [7], pentostatin [8], ophicordin [9], beauvericin [10], myriocin [11], beauveriolide [12], and oosporein [13] (Figure 1). These bioactive components function in various ways.…”