Integrative analyses of gene expression and alternative splicing to gain insights into the effects of copper on hepatic lipid metabolism in swamp eel (Monopterus albus)

Liu, Lin; Zhou, Qiubai; Lin, Changgao; He, Li; Wei, Lili

doi:10.1016/j.aquaculture.2021.737367

Cited by 12 publications

(6 citation statements)

References 75 publications

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“…The 56-day feeding trial were conducted according to our previous study with some modification ( 11 ). Each diet group was randomly assigned to four replicates, and one replicate corresponded to one tank (100 cm × 60 cm × 50 cm, 20 healthy swamp eels per tank with an initial weight of 12.10 ± 0.03 g).…”

Section: Methodsmentioning

confidence: 99%

“…Swamp eel ( Monopterus albus ) is a high-value commercially farmed, eurythermal, and freshwater carnivorous fish with a desirable growth rate, good meat quality, and an exceptional ability to obtain oxygen from the air rather than water ( 11 , 12 ). Our previous study showed that a high-carbohydrate diet resulted in liver injury in swamp eel ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

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Dietary Macroalgae Saccharina japonica Ameliorates Liver Injury Induced by a High-Carbohydrate Diet in Swamp Eel (Monopterus albus)

Wang

Cai

et al. 2022

Front. Vet. Sci.

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A high-carbohydrate diet lowers the rearing cost and decreases the ammonia emission into the environment, whereas it can induce liver injury, which can reduce harvest yields and generate economic losses in reared fish species. Macroalgae Saccharina japonica (SJ) has been reported to improve anti-diabetic, but the protective mechanism of dietary SJ against liver injury in fish fed a high-carbohydrate diet has not been studied. Therefore, a 56-day nutritional trial was designed for swamp eel Monopterus albus, which was fed with the normal diet [20% carbohydrate, normal carbohydrate (NC)], a high carbohydrate diet (32% carbohydrate, HC), and a HC diet supplemented with 2.5% SJ (HC-S). The HC diet promoted growth and lowered feed coefficient (FC), whereas it increased hepatosomatic index (HSI) when compared with the NC diet in this study. However, SJ supplementation increased iodine contents in muscle, reduced HSI, and improved liver injury, such as the decrease of glucose (GLU), total bile acid (TBA), and alanine aminotransferase (ALT) in serum, and glycogen and TBA in the liver. Consistently, histological analysis showed that SJ reduced the area of lipid droplet, glycogen, and collagen fiber in the liver (p < 0.05). Thoroughly, the underlying protective mechanisms of SJ supplementation against HC-induced liver injury were studied by liver transcriptome sequencing coupled with pathway analysis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differentially expressed genes (DEGs), such as the acetyl-coenzyme A synthetase (acss1), alcohol dehydrogenase (adh), interferon-induced protein with tetratricopeptide repeats 1 (ifit1), aldo-keto reductase family 1 member D1 (akr1d1), cholesterol 7-alpha-monooxygenase (cyp7a1), and UDP-glucuronosyltransferase (ugt), indicated that the pathway of glycolysis/gluconeogenesis was the main metabolic pathway altered in the HC group compared with the NC group. Meanwhile, hepatitis C, primary BA biosynthesis, and drug metabolism-cytochrome P450 were the three main metabolic pathways altered by SJ supplementation when compared with the HC group. Moreover, the BA-targeted metabolomic analysis of the serum BA found that SJ supplementation decreased the contents of taurohyocholic acid (THCA), taurochenodeoxycholic acid (TCDCA), taurolithocholic acid (TLCA), nordeoxycholic acid (NorDCA), and increased the contents of ursocholic acid (UCA), allocholic acid (ACA), and chenodeoxycholic acid (CDCA). In particular, the higher contents of UCA, ACA, and CDCA regulated by SJ were associated with lower liver injury. Overall, these results indicate that the 2.5% supplementation of SJ can be recommended as a functional feed additive for the alleviation of liver injury in swamp eel-fed high-carbohydrate diets.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary Macroalgae Saccharina japonica Ameliorates Liver Injury Induced by a High-Carbohydrate Diet in Swamp Eel (Monopterus albus)

Wang

Cai

et al. 2022

Front. Vet. Sci.

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“…The primary pathways implicated in these processes include the uptake of circulating lipids, de novo lipogenesis, and fatty acid oxidation. First, compared to common grass carp, higher blood levels of TG, TC, HDL‐C, and LDL‐C as well as up‐regulation of fabp7a, a transporter fatty acid transporter protein (Liu et al., 2022), were observed in CGC liver, suggesting increased lipid uptake. Additionally, de novo lipogenesis, which involves the synthesis of fatty acids from carbohydrate sources, primarily glucose, contributes to at least 35% of lipids in steatotic cells (Gnoni et al., 2022).…”

Section: Discussionmentioning

confidence: 99%

Broad bean (Vicia faba L.) caused abnormal lipid metabolism in grass carp (Ctenopharyngodon idellus) liver: Insight from the gut microbiota–liver axis

Fu,

Ma,

Yang

et al. 2024

Food Frontiers

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Broad bean (Vicia faba L.) has received attention from the food and animal feed industries as a sustainable, inexpensive, and versatile plant‐based protein source. However, long‐term consumption of broad bean, especially unprocessed ones, can lead to hepatic lipid accumulation. To investigate how broad bean affected hepatic lipid metabolism and its underlying mechanisms, we conducted a multi‐omics analysis of crisp grass carp (Ctenopharyngodon idellus C. et V) model with hepatic steatosis after feeding with single broad bean for 120 days. The glycolysis inhibition, increased de novo lipogenesis, abnormalities in lipid metabolism, and activation of oxidative stress were the key processes involved in broad bean‐induced hepatic lipid accumulation. Disturbances in intestinal flora caused by damage to the intestinal barrier and increased lipopolysaccharide caused by broad bean promoted hepatic lipid accumulation. Reduction of antinutritional factors and balanced diet offer potential strategies to mitigate the liver damage caused by broad bean consumption. Overall, our findings provided a theoretical basis for the wide application of broad bean as animal feeds.

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“…However, their specific molecular mechanisms required further study in the future. Additionally, functional genes with diverse AS events were also detected in other organisms, such as the Na + /K + /2Cl − cotransporter in humans [ 43 ], signal transducer and activator of transcription 3 in zebrafish [ 44 ], immunoglobulin heavy chain in spotted sea bass [ 9 ] and acetyl-CoA carboxylase alpha in swamp eels ( Monopterus albus ) [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Alternative Splicing (AS) Mechanism Provides Insights into Salinity Adaptation in the Livers of Three Euryhaline Teleosts, including Scophthalmus maximus, Cynoglossus semilaevis and Oncorhynchus mykiss

Tian

Gao

Dong

et al. 2022

Biology

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Salinity is an important environmental factor that directly affects the survival of aquatic organisms, including fish. However, the underlying molecular mechanism of salinity adaptation at post-transcriptional regulation levels is still poorly understood in fish. In the present study, 18 RNA-Seq datasets were utilized to investigate the potential roles of alternative splicing (AS) in response to different salinity environments in the livers of three euryhaline teleosts, including turbot (Scophthalmus maximus), tongue sole (Cynoglossus semilaevis) and steelhead trout (Oncorhynchus mykiss). A total of 10,826, 10,741 and 10,112 AS events were identified in the livers of the three species. The characteristics of these AS events were systematically investigated. Furthermore, a total of 940, 590 and 553 differentially alternative splicing (DAS) events were determined and characterized in the livers of turbot, tongue sole and steelhead trout, respectively, between low- and high-salinity environments. Functional enrichment analysis indicated that these DAS genes in the livers of three species were commonly enriched in some GO terms and KEGG pathways associated with RNA processing. The most common DAS genes work as RNA-binding proteins and play crucial roles in the regulation of RNA splicing. The study provides new insights into uncovering the molecular mechanisms of salinity adaptation in teleosts.

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Integrative analyses of gene expression and alternative splicing to gain insights into the effects of copper on hepatic lipid metabolism in swamp eel (Monopterus albus)

Cited by 12 publications

References 75 publications

Dietary Macroalgae Saccharina japonica Ameliorates Liver Injury Induced by a High-Carbohydrate Diet in Swamp Eel (Monopterus albus)

Dietary Macroalgae Saccharina japonica Ameliorates Liver Injury Induced by a High-Carbohydrate Diet in Swamp Eel (Monopterus albus)

Broad bean (Vicia faba L.) caused abnormal lipid metabolism in grass carp (Ctenopharyngodon idellus) liver: Insight from the gut microbiota–liver axis

Genome-Wide Analysis of Alternative Splicing (AS) Mechanism Provides Insights into Salinity Adaptation in the Livers of Three Euryhaline Teleosts, including Scophthalmus maximus, Cynoglossus semilaevis and Oncorhynchus mykiss

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