Integrative analyses of<i>Nervilia</i>(Orchidaceae) section<i>Linervia</i>reveal further undescribed cryptic diversity in Thailand

Gale, Stephan W.; Duangjai, Sutee; Li, Jihong; Yu, I.; Watthana, Santi; Termwutthipreecha, Phatsara; Cheuk, Mang Lung; Suddee, Somran

doi:10.1080/14772000.2017.1415233

Cited by 10 publications

(8 citation statements)

References 66 publications

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“…Species complexes are common across the orchid family (e.g. Bateman et al, 2017;Gale et al, 2018;Johnson and Steiner, 1997). As such, orchids provide a good opportunity to test the utility of GBS data for resolving phylogenetic relationships among taxa with little or overlapping morphological variation.…”

Section: Introductionmentioning

confidence: 99%

Resolving relationships in an exceedingly young orchid lineage using Genotyping-by-sequencing data

Escobar

Bogarín

Schley

et al. 2018

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Poor morphological and molecular differentiation in recently diversified lineages is a widespread phenomenon in plants. Phylogenetic relationships within such species complexes are often difficult to resolve because of the low variability in traditional molecular loci, as well as various other biological phenomena responsible for topological incongruence such as ILS and hybridization. In this study, we employ a Genotyping-by-sequencing (GBS) approach to disentangle evolutionary relationships within a species complex belonging to the Neotropical orchid genus Cycnoches. The complex includes seven taxa distributed in Central America and the adjacent Chocó biogeographic region, nested within a clade estimated to have first diversified in the early Quaternary. Previous phylogenies inferred from a handful of loci failed to provide support for internal relationships within the complex. Our Neighbor-net and coalescent-based analyses inferred from ca. 13,000 GBS loci obtained from 31 individuals belonging to six of the seven traditionally accepted Cycnoches species provided a robustly supported network. The resulting three main clades are corroborated by morphological traits and geographical distributions. Similarly, Maximum Likelihood (ML) inferences of concatenated GBS-loci produced results comparable with those derived from coalescence and network-based methods, albeit always with poor statistical support. The low support evident in the ML phylogeny might be attributed to the abundance of uninformative GBS loci, which can account for up to 50% of the total number of loci recovered. The phylogenomic framework provided here, as well as morphological evidence and geographical patterns, suggest that the six entities previously thought to be different species might actually represent only three distinct segregates. Our study is the first to demonstrate the utility of GBS data in phylogenomic research of a very young Neotropical plant clade (~2 Ma), and it paves the way for the study of the many other species complexes that populate the species-rich orchid family.

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Section: Introductionmentioning

confidence: 99%

Resolving relationships in an exceedingly young orchid lineage using Genotyping-by-sequencing data

Escobar

Bogarín

Schley

et al. 2018

Preprint

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“…is a genus of some 75–80 accepted species occurring in the tropics and subtropics of Africa, Asia and Oceania (Govaerts et al., 2020). Most species remain poorly known taxonomically due to a combination of their rarity, ephemeral emergence and inconspicuous habit (Gale et al., 2018). Moreover, the hysteranthous growth pattern, a diagnostic feature of the genus, means that generative and vegetative shoots are rarely present at the same time.…”

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“…three or more) flowered inflorescence are placed in section Nervilia, those with a two‐flowered inflorescence are placed in section Vinerlia and those with a one‐flowered inflorescence are placed in section Linervia; the latter now also includes one‐flowered species with a spurred lip previously assigned to section Kyimbilaea (Pridgeon et al., 2005). Although both cladistic analysis of morphological data (Pettersson, 1991) and phylogenetic analysis of molecular data (Gale et al., 2018) provide support for this infrageneric classification, several exceptions exist throughout the range of the genus, debasing the utility of flower number per se in assigning species to their appropriate section. Instead, correlated details of floral anatomy, principally lip outline and ornamentation, as well as leaf shape and pubescence, are more informative (Gale, 2009; Gale et al., 2018).…”

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“…Although both cladistic analysis of morphological data (Pettersson, 1991) and phylogenetic analysis of molecular data (Gale et al., 2018) provide support for this infrageneric classification, several exceptions exist throughout the range of the genus, debasing the utility of flower number per se in assigning species to their appropriate section. Instead, correlated details of floral anatomy, principally lip outline and ornamentation, as well as leaf shape and pubescence, are more informative (Gale, 2009; Gale et al., 2018). In addition, elongation of the scape during fructification appears to be a synapomorphy for section Linervia, to which N. nipponica belongs (Pettersson, 1991).…”

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“…In addition, elongation of the scape during fructification appears to be a synapomorphy for section Linervia, to which N. nipponica belongs (Pettersson, 1991). All three sections contain species complexes within which leaf morphology is largely uniform but minor differences in floral morphology sometimes conceals wide genetic and cytological divergence between cryptic taxa (Gale et al., 2015, 2018; Ketjarun et al., 2019). Nervilia nipponica is referable to the ‘ Nervilia adolphi / punctata species alliance’, a monophyletic complex of approximately 35 diminutive, one‐flowered species characterised by an entire, slender, white and purple‐marked labellum that lacks a spur and a glabrous, angular‐cordate leaf (Gale et al., 2018; Gale & Phaxaysombath, 2017; Pettersson, 1991).…”

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International Biological Flora: Nervilia nipponica

Gale¹,

Maeda

Miyashita

et al. 2021

Journal of Ecology

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This account presents information on all aspects of the biology of Nervilia nipponica Makino (mukago‐saishin) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the International Biological Flora: distribution, habitat, communities, responses to biotic factors, responses to the environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history, conservation and global heterogeneity. Nervilia nipponica is a small, stoloniferous, seasonally dormant herb that grows in the understorey of evergreen forests in the humid subtropical zone of central and western Japan, with a few outlying populations on Jeju Island in South Korea. Its northern extent is defined by the 0°C winter isotherm, and its occurrence is also limited by site aspect and incline. It is a weak competitor that occupies species‐poor microsites in which bare ground and leaf litter predominate. Plant numbers tend to decline as percentage ground cover of surrounding understorey vegetation increases. The inflorescence sprouts from a short‐lived, subterranean tuber in late spring and leaf‐flush occurs after fruit‐set. However, most tubers do not flower in any one annual growth cycle. Long‐term monitoring of individually marked plants suggests that tubers are resource‐limited and that flowering constrains future genet growth. Nervilia nipponica is exclusively autogamous and has a strong capacity for vegetative propagation. The species is genetically depauperate but exhibits significant differentiation between populations, which comprise clonal clusters in phalanx formation. The level of mycorrhizal infection differs between plant parts and through successive phenological stages. Stable isotope signatures indicate that the species is partially mycoheterotrophic, with fungal partners supporting growth particularly at lower light intensities. Despite this, falling light availability associated with forest succession can lead to population decline. Populations tend to be small and prone to extirpation, but the species is probably under‐recorded as a result of its ephemeral emergence above‐ground and inconspicuous habit. Management interventions likely to benefit the species at the site level include thinning dense forest canopy and removing encroaching ground cover.

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Studies in Asian Nervilia (Orchidaceae) VIII: N. hemratii, another new member of section Linervia from Thailand

Gale¹,

Tetsana

Suddee

2022

Kew Bull

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Integrative analyses ofNervilia(Orchidaceae) sectionLinerviareveal further undescribed cryptic diversity in Thailand

Cited by 10 publications

References 66 publications

Resolving relationships in an exceedingly young orchid lineage using Genotyping-by-sequencing data

Resolving relationships in an exceedingly young orchid lineage using Genotyping-by-sequencing data

International Biological Flora: Nervilia nipponica

Studies in Asian Nervilia (Orchidaceae) VIII: N. hemratii, another new member of section Linervia from Thailand

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