Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis

Abstract

Excessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.

Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

“…However, in-depth studies on the functions of chicken circFNDC3AL and circHSPG2 on adipogenic differentiation are essential. Previous research reported that runtrelated transcription factor 1 (RUNX1T1) [42,43], myosin, heavy chain 9, non-muscle (MYH9), fatty acid desaturase 2 (FADS2), insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) were associated with adipogenesis and fat deposition [44][45][46][47]. We found that circLCLAT1 might influence adipogenic differentiation by regulating downstream genes (RUNX1T1, FADS2, MYH9, IGF2BP3, and PDGFRA) through four miRNAs (miR-34a-5p, miR-30e-5p, miR-146b-5p, and miR-147).…”

Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

“…However, indepth studies on the functions of chicken circFNDC3AL and circHSPG2 on adipogenic differentiation are essential. Previous research reported that runt-related transcription factor 1 (RUNX1T1) [42,43], myosin, heavy chain 9, non-muscle (MYH9), fatty acid desaturase 2 (FADS2), insulin-like growth factor 2 mRNAbinding protein 1 (IGF2BP1) were associated with adipogenesis and fat deposition [44][45][46][47]. We found that circLCLAT1 might in uence adipogenic differentiation by regulating downstream genes (RUNX1T1, FADS2, MYH9, IGF2BP3, and PDGFRA) through four miRNAs (miR-34a-5p, miR-30e-5p, miR-146b-5p, and miR-147).…”

Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

“…Further studies need to be done to investigate the functions of chicken circFNDC3AL and circHSPG2 on adipogenic differentiation. Previous studies reported that runt-related transcription factor 1 (RUNX1T1) [42,43], myosin, heavy chain 9, nonmuscle (MYH9), fatty acid desaturase 2 (FADS2), insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) [44][45][46][47] were associated with adipogenesis and fat deposition. We found that circLCLAT1 might affect adipogenic differentiation by regulating downstream genes (RUNX1T1, FADS2, MYH9, IGF2BP1 and PDGFRA) via four miRNAs (miR-34a-5p, miR-30e-5p, miR-146b-5p and miR-147).…”