Integrative analysis based on transcriptome revealed the relation of auxin to turnip hypocotyl-tuber formation

Huang, Yingjuan; Liu, Ruicheng; Song, Ruiqi; Zhou, Dong; Chen, Jingwen; Liu, Rui; Chen, Weiwei; Yang, Jianli; Yu, Xiaolin; Cao, Jiashu; Huang, Li

doi:10.1016/j.scienta.2022.111536

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…Primer 5.0 software was used to design primers and verify their specificity in the Brassica Database (http://brassicadb.cn/) (accessed on 4 March 2023). BraUBC10 was used for the internal reference gene [55]. The primer sequence information is shown in Table S7.…”

Section: Rna Extraction and Real-time Quantitative Pcr Assay (Qpcr)mentioning

confidence: 99%

Expression Characterization of ABCDE Class MADS-Box Genes in Brassica rapa with Different Pistil Types

Zhang,

Zhao,

Wang

et al. 2023

Plants

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MADS-box is a vital transcription factor family that functions in plant growth and development. Apart from APETALA2, all genes in the ABCDE model that explain the molecular mechanism of floral organ development belong to the MADS-box family. Carpel and ovule numbers in plants are essential agronomic traits that determine seed yield, and multilocular siliques have great potential for the development of high-yield varieties of Brassica. In this study, ABCDE genes in the MADS-box family from Brassica rapa were identified and characterized. Their tissue-specific expression patterns in floral organs and their differential expression in different pistil types of B. rapa were revealed by qRT-PCR. A total of 26 ABCDE genes were found to belong to the MADS-box family. Our proposed ABCDE model of B. rapa is consistent with that of Arabidopsis thaliana, indicating that ABCDE genes are functionally conserved. These results of qRT-PCR showed that the expression levels of class C and D genes were significantly different between the wild-type (wt) and tetracarpel (tetrac) mutant of B. rapa. Interestingly, the expression of the homologs of class E genes was imbalanced. Therefore, it is speculated that class C, D, and E genes are involved in developing the carpel and ovule of B. rapa. Our findings reveal the potential for the selection of candidate genes to improve yield traits in Brassica crops.

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Section: Rna Extraction and Real-time Quantitative Pcr Assay (Qpcr)mentioning

confidence: 99%

Expression Characterization of ABCDE Class MADS-Box Genes in Brassica rapa with Different Pistil Types

Zhang,

Zhao,

Wang

et al. 2023

Plants

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Genome-wide identification and expression analysis of the AUX/IAA gene family in turnip (Brassica rapa ssp. rapa)

Liu

Zhang

et al. 2023

BMC Plant Biol

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Background Auxin/indoleacetic acid (AUX/IAA) genes encoding short-lived proteins participate in AUX signaling transduction and play crucial roles in plant growth and development. Although the AUX/IAA gene family has been identified in many plants, a systematic analysis of AUX/IAA genes in Brassica rapa ssp. rapa has not yet been reported. Results We performed a comprehensive genome-wide analysis and found 89 AUX/IAA genes in turnip based on the conserved AUX/IAA domain (pfam02309). Phylogenetic analysis of AUX/IAA genes from turnip, Arabidopsis, and cabbage revealed that these genes cluster into six subgroups (A1, A2, A3, A4, B1, and B2). The motif distribution was also conservative among the internal members of the clade. Enhanced yellow fluorescent protein (EYFP) signals of BrrIAA-EYFPs showed that BrrIAA members functioned as nucleoproteins. Moreover, transcriptional analysis revealed that the expression patterns of AUX/IAA genes in turnip were tissue-dependent. Because orthologs have similar biological functions and interaction networks in plant growth and development, BrrIAA66 in turnip possibly played a role in embryo axis formation, vascular development, lateral root formation, and floral organ development by interacting with BrrARF19 and BrrTIR1. Conclusion These results provide a theoretical basis for further investigation of BrrAUX/IAA genes and lay the foundation for functional analysis of BrrIAA66 in turnip.

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Integrative analysis based on transcriptome revealed the relation of auxin to turnip hypocotyl-tuber formation

Cited by 2 publications

References 40 publications

Expression Characterization of ABCDE Class MADS-Box Genes in Brassica rapa with Different Pistil Types

Expression Characterization of ABCDE Class MADS-Box Genes in Brassica rapa with Different Pistil Types

Genome-wide identification and expression analysis of the AUX/IAA gene family in turnip (Brassica rapa ssp. rapa)

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