Integrative analysis identifies the safety of transgenic Cry1Ab rice to non-target spider Pardosa pseudoannulata

RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata

“…The reference gene used was the 18S ribosomal RNA of Pardosa pseudoannulata. 41,42 RT-qPCR used the following reaction conditions: an initial denaturation at 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s, 60 °C for 30 s and 72 °C for 30 s. A temperature of 95 °C for 30 s was used for melting curve analysis, and to ensure the consistency and specificity of the amplified products. Normalization of cDNA levels was performed using 18S ribosomal RNA and the 2 −ΔΔCt method for analysis.…”

Communal rearing induces high predatory capacity in a solitary wolf spider and its potential in pest control

“…To date, there is little published research on the issue that whether early rearing experience of solitary spiders would affect their later foraging behavior. Pardosa pseudoannulata (Araneae: Lycosidae) is a wandering wolf spider that has large populations in rice fields in Asia, and it is a major predator of pests such as Nilaparvata lugens in the rice field (Lv et al, 2022 ; Tang et al, 2022 ). The planthopper N. lugens (Stål) (Hemiptera: Delphacidae) is one of the most serious pests of rice in both temperate and tropical regions of east and south Asia, and causes serious yield losses of rice crops all over the world (Liu, Li, et al, 2020 ; Liu, Zhuang, et al, 2020 ; Riley et al, 1991 ).…”