Integrative analysis of exogenous auxin mediated plant height regulation in Moso bamboo (Phyllostachys edulis)

Bai, Yucong; Cai, Miaomiao; Mu, Changhong; Zheng, Huifang; Cheng, Zhanchao; Xie, Yali; Gao, Jian

doi:10.1016/j.indcrop.2023.116852

Cited by 12 publications

(9 citation statements)

References 47 publications

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“…In Moso bamboo, previous studies have found that the phytohormone content is not the same in different parts of the same internode in bamboo shoots, and that the content of auxin, gibberellin and cytokinin is much higher at the base of the internode than at the upper part of the internode [ 41 ]. At the same time, we have identified the role of auxin and gibberellin in the regulation of the internode length and plant height, and the possible involvement of cytokinin in the regulation of elongation in the internode elongation zone of Moso bamboo, but the molecular mechanisms involved are not yet clear [ 29 , 30 , 42 ]. In order to further analyze the integration of phytohormone signaling in Moso bamboo, we found that most of the genes containing the corresponding regulatory elements in the promoter region could respond to the corresponding phytohormones based on the available transcriptome data, and further determined the phytohormone co-expression pattern during the developmental stage of bamboo shoots.…”

Section: Discussionmentioning

confidence: 99%

“…During the entire growth period, plant hormones such as auxin, cytokinin and gibberellin play a crucial role. Gibberellin and auxin directly influence the internode length in Moso bamboo, while the dynamic balance between the cytokinin and auxin content affects internode cell elongation [ 29 , 30 ]. Here, we systematically identified gene families critical for CK synthesis and signal transduction, and conducted a preliminary investigation into the regulation of cytokinin and auxin, laying the foundation for studying the involvement of CK in the regulation of Moso bamboo growth.…”

Section: Introductionmentioning

confidence: 99%

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Phytohormone Crosstalk of Cytokinin Biosynthesis and Signaling Family Genes in Moso Bamboo (Phyllostachys edulis)

Bai

Cai

Dou

et al. 2023

IJMS

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Cytokinin is widely involved in the regulation of plant growth, but its pathway-related genes have not been reported in Moso bamboo. In this study, a total of 129 candidate sequences were identified by bioinformatic methods. These included 15 IPT family genes, 19 LOG family genes, 22 HK family genes, 11 HP family genes and 62 RR family genes. Phylogenetic analysis revealed that the cytokinin pathway was closely related to rice, and evolutionary pattern analysis found that most of the genes have syntenic relationship with rice-related genes. The Moso bamboo cytokinin pathway was evolutionarily conservative and mainly underwent purifying selection, and that gene family expansion was mainly due to whole-gene duplication events. Analysis of transcriptome data revealed a tissue-specific expression pattern of Moso bamboo cytokinin family genes, with auxin and gibberellin response patterns. Analysis of co-expression patterns at the developmental stages of Moso bamboo shoots revealed the existence of a phytohormone co-expression pattern centered on cytokinin signaling genes. The auxin signaling factor PheARF52 was identified by yeast one-hybrid assay as regulating the PheRR3 gene through a P-box element in the PheRR3 promoter region. Auxin and cytokinin signaling crosstalk to regulate Moso bamboo growth. Overall, we systematically identified and analyzed key gene families of the cytokinin pathway in Moso bamboo and obtained key factors for auxin and cytokinin crosstalk, laying the foundation for the study of hormone regulation in Moso bamboo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phytohormone Crosstalk of Cytokinin Biosynthesis and Signaling Family Genes in Moso Bamboo (Phyllostachys edulis)

Bai

Cai

Dou

et al. 2023

IJMS

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“…Bamboo shoots were selected 50 cm tall in the bamboo forest for basal injections of exogenous sugars and hormones every three days and sampled after fifteen days for transcriptome sequencing. Auxin related bamboo shoot transcriptome data are stored in the SAR database under access number PRJNA788576 [ 36 ]. Heat maps presented in this study were generated using TBtools software.…”

Section: Methodsmentioning

confidence: 99%

Deciphering the regulatory role of PheSnRK genes in Moso bamboo: insights into hormonal, energy, and stress responses

Zheng,

Xie,

et al. 2024

BMC Genomics

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The SnRK (sucrose non-fermentation-related protein kinase) plays an important role in regulating various signals in plants. However, as an important bamboo shoot and wood species, the response mechanism of PheSnRK in Phyllostachys edulis to hormones, low energy and stress remains unclear. In this paper, we focused on the structure, expression, and response of SnRK to hormones and sugars. In this study, we identified 75 PheSnRK genes from the Moso bamboo genome, which can be divided into three groups according to the evolutionary relationship. Cis-element analysis has shown that the PheSnRK gene can respond to various hormones, light, and stress. The PheSnRK2.9 proteins were localized in the nucleus and cytoplasm. Transgenic experiments showed that overexpression of PheSnRK2.9 inhibited root development, the plants were salt-tolerant and exhibited slowed starch consumption in Arabidopsis in the dark. The results of yeast one-hybrid and dual luciferase assay showed that PheIAAs and PheNACs can regulate PheSnRK2.9 gene expression by binding to the promoter of PheSnRK2.9. This study provided a comprehensive understanding of PheSnRK genes of Moso bamboo, which provides valuable information for further research on energy regulation mechanism and stress response during the growth and development of Moso bamboo.

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“…These findings suggest that Moso bamboo exhibits an intricate adaptation mechanism to modify phenotypic traits when faced with stress conditions. Additionally, understanding its fast growth process is crucial for rapid biomass production [ 28 , 29 , 30 ]. Thus, significant improvement has been made in understanding its culm development, which is a well-defined process involving various stages [ 8 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Culm Morphological Analysis in Moso Bamboo Reveals the Negative Regulation of Internode Diameter and Thickness by Monthly Precipitation

Zhang,

Chu,

Gao

et al. 2024

Plants

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The neglect of Moso bamboo’s phenotype variations hinders its broader utilization, despite its high economic value globally. Thus, this study investigated the morphological variations of 16 Moso bamboo populations. The analysis revealed the culm heights ranging from 9.67 m to 17.5 m, with average heights under the first branch ranging from 4.91 m to 7.67 m. The total internode numbers under the first branch varied from 17 to 36, with internode lengths spanning 2.9 cm to 46.4 cm, diameters ranging from 5.10 cm to 17.2 cm, and wall thicknesses from 3.20 mm to 33.3 mm, indicating distinct attributes among the populations. Furthermore, strong positive correlations were observed between the internode diameter, thickness, length, and volume. The coefficient of variation of height under the first branch showed strong positive correlations with several parameters, indicating variability in their contribution to the total culm height. A regression analysis revealed patterns of covariation among the culm parameters, highlighting their influence on the culm height and structural characteristics. Both the diameter and thickness significantly contribute to the internode volume and culm height, and the culm parameters tend to either increase or decrease together, influencing the culm height. Moreover, this study also identified a significant negative correlation between monthly precipitation and the internode diameter and thickness, especially during December and January, impacting the primary thickening growth and, consequently, the internode size.

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Integrative analysis of exogenous auxin mediated plant height regulation in Moso bamboo (Phyllostachys edulis)

Cited by 12 publications

References 47 publications

Phytohormone Crosstalk of Cytokinin Biosynthesis and Signaling Family Genes in Moso Bamboo (Phyllostachys edulis)

Phytohormone Crosstalk of Cytokinin Biosynthesis and Signaling Family Genes in Moso Bamboo (Phyllostachys edulis)

Deciphering the regulatory role of PheSnRK genes in Moso bamboo: insights into hormonal, energy, and stress responses

Culm Morphological Analysis in Moso Bamboo Reveals the Negative Regulation of Internode Diameter and Thickness by Monthly Precipitation

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