“…Long-read sequencing enables accurate mapping of diverse RNA features with transcript isoform resolution. Such features include, among others, chemical modifications (Acera Mateos et al, 2024;Bansal et al, 2024;Liu et al, 2019;Stephenson et al, 2020), structured regions (Aw et al, 2021;Stephenson et al, 2022), and RNA-protein interacting sites (Lin et al, 2022). The functional roles of these RNA features are often linked to their position relative to transcript landmarks, such as transcription start sites, splice junctions, start and stop codons, and polyadenylation sites.…”