Integrative analysis of the gastric cancer long non‑coding RNA‑associated competing endogenous RNA network

Jiang, Yuyou; Zhang, Xianqin; Li, Rong; Hou, Yi; Jing, Song; Zhang, Wanfeng; He, Min; Xie, Yiqiang; Li, Yue; Song, Fangzhou

doi:10.3892/ol.2021.12717

Cited by 7 publications

(6 citation statements)

References 52 publications

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“… 55 , 56 LINC00501 , as an upregulated oncogenic lncRNA, was reported to distinguish GC from normal stomach tissues. 23 Consistent with this previous study, we reported that LINC00501 was significantly upregulated in GC tissues than in normal stomach tissues. Importantly, LINC00501 exhibited the further potential to identify advanced and metastatic GC; the AUCs of the ROC curve were 0.767 and 0.737, respectively.…”

Section: Discussionsupporting

confidence: 91%

“…Emerging studies reported that lncRNA is a potential candidate biomarker for GC 55,56 . LINC00501 , as an upregulated oncogenic lncRNA, was reported to distinguish GC from normal stomach tissues 23 . Consistent with this previous study, we reported that LINC00501 was significantly upregulated in GC tissues than in normal stomach tissues.…”

Section: Discussionsupporting

confidence: 91%

“…Overall, 1866 upregulated and 2466 downregulated lncRNAs were identified. LINC00501, one of the top 5 dysregulated lncRNAs, displayed a significant 10.91-fold elevation in its expression level when compared to the surrounding noncancerous tissues, considering LINC00501 has been reported to show the potential to become gastric cancer biomarker, 23 this result sparked our interest for further study (Supplementary Figure S1A). The RNA characteristics of LINC00501 were also analysed and indicated LINC00501 exhibited low-coding potential and high expression in GI cancers (Supplementary Figure S2A-C).…”

Section: Linc00501 Correlates With Advanced Gc Stage and Metastasismentioning

confidence: 99%

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Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial‐mesenchymal transition and angiogenesis

Dou,

Han,

Yang

et al. 2023

Clinical & Translational Med

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BackgroundThe molecular mechanism of the significant role of long noncoding RNAs (lncRNAs) in the progression and metastasis of gastric cancer (GC) remains largely elusive. Our objective is to detect overexpressed lncRNA in GC and investigate its role in promoting epithelial‐mesenchymal transition and tumour microenvironment remodel.MethodsLncRNA differential expression profile in GC was analysed using RNA microarrays. The level of LINC00501 was evaluated in both GC patient tissues and GC cell lines by quantitative reverse transcription PCR and large‐scale (n = 304) tissue microarray. To explore the biological role and regulatory driver of LINC00501 in GC, various experimental techniques including Chromatin isolation by RNA purification (ChIRP), RNA immunoprecipitation (RIP), chromatin immunoprecipitation (ChIP) assay, dual luciferase assays were performed.ResultsClinically, it was observed that LINC00501 level was abnormal overexpression in GC tissue and was associated with GC progression and distant metastasis. Gain and loss molecular biological experiments suggested that LINC00501, promoted EMT process and angiogenesis of GC. Mechanically, the enrichment of H3K27 acetylation in LINC00501 promoter region contributed to the increase of LINC00501 in GC. LINC00501 transactivated transcription of SLUG, by recruiting hnRNPR to its promoter. The growth of GC was inhibited both in vitro and in vivo by suppressing the level of LINC00501 using pharmacological intervention from the histone acetyltransferase (HAT) inhibitor ‐C646.ConclusionsThis study suggests that LINC00501 promotes GC progression via hnRNPR/SLUG pathway, which indicates a promising biomarker and target for GC.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Linc00501 Correlates With Advanced Gc Stage and Metastasismentioning

confidence: 99%

See 1 more Smart Citation

Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial‐mesenchymal transition and angiogenesis

Dou,

Han,

Yang

et al. 2023

Clinical & Translational Med

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“…A recent study demonstrated that GK-IT1 was a potential diagnostic biomarker for gastric cancer through bioinformatics analysis. 38 Our study demonstrated the clinical implication of GK-IT1 in ESCC. In addition, we showed that GK-IT1 could promote the proliferation and metastasis of ESCC cells for the first time.…”

Section: Discussionsupporting

confidence: 53%

Long noncoding RNA GK‐IT1 promotes esophageal squamous cell carcinoma by regulating MAPK1 phosphorylation

et al. 2022

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Background Long noncoding RNAs (lncRNAs) are implicated in the oncogenesis and metastasis of multiple human cancers. Nonetheless, the precise molecular mechanisms underlying the oncogenic role of lncRNA in esophageal squamous cell carcinoma (ESCC) remains to be clarified. Methods The expression of GK intronic transcript 1 (GK‐IT1) was analyzed using ESCC RNA‐seq data from The Cancer Genome Atlas database. Quantitative real‐time PCR was used to measure the expression of GK‐IT1 in ESCC clinical samples and cells. The correlation between GK‐IT1 expression and clinicopathological variables was examined using chi‐squared tests. Kaplan–Meier survival and Cox regression analyses were employed to generate the survival curve and assess the prognostic value of GK‐IT1. Functional experiments were utilized to explore the role of GK‐IT1 in promoting cell migration, invasion, proliferation, and suppressing apoptosis and autophagy in ESCC. To understand the mechanism, an RNA pulldown assay, RNA immunoprecipitation, agarose gel electrophoresis, immunofluorescence, and co‐immunoprecipitation assays were used. Results In this study we identified an unreported lncRNA, termed GK‐IT1 that was aberrantly overexpressed in ESCC tissues and cells. GK‐IT1 was closely associated with advanced clinical stage, and it was an independent prognostic indicator of ESCC. Functional assays verified that GK‐IT1 significantly promoted ESCC proliferation, invasion, and migration, and suppressed ESCC apoptosis and autophagy. Furthermore, tumorigenesis experiments in nude mice indicated that GK‐IT1 promoted ESCC tumor growth and metastasis. Mechanistically, GK‐IT1 competitively bound to mitogen‐activated protein kinase 1 (MAPK1) to prevent the interaction between dual specificity phosphatase 6 (DUSP6) and MAPK1, thereby controlling the phosphorylation of MAPK1 and promoting ESCC progression. Conclusion Our study revealed that GK‐IT1 competed with DUSP6 to attenuate the interaction between DUSP6 and MAPK1, leading to activation of the ERK/MAPK pathway, thereby promoting progression of ESCC. Our research indicated that GK‐IT1 served as a novel potential target for the diagnosis and treatment of ESCC.

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“…Overexpression of FGF14-AS2 impaired the miR-205-5p induced phenotypic characteristics on proliferation, invasion, migration, and apoptosis in breast cancer cells [ 26 ]. It is identified that LINC01122 (cg09671962) is closely associated with OS coupled with a high risk of gastric cancer [ 27 ]. It was proved that MEG3 (cg14245102) can affect Wnt-β-catenin pathway and control epithelial-mesenchymal transition [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

A DNA methylation profile of long non-coding RNAs can predict OS in prostate cancer

et al. 2021

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Prostate cancer (PCa) is the most common male reproductive tract malignant tumor, accurate evaluation of PCa characterization and prognostic prediction at diagnosis are vital for the effective administration of the disease, especially at the molecular level. In this study, 48 CpG sites with differential methylation associated with overall survival (OS) were screened out between PCa and normal adjacent tissues. 16 CpG sites were selected by the least absolute shrinkage and selection operator (LASSO) and the risk score formula for methylated-based classifier was established. For 16-lncRNAs-CpG-classifier, the area under the curve (AUC) were 0.890, 0.917, and 0.932 at 3 years, 5 years and 7 years, respectively. Kaplan-Meier curves indicated that patients with high-risk scores had worse OS than those with low-risk scores. Prognostic methylation model of lncRNAs was identified from the whole genome in patients with PCa. This novel finding provides a novel insight for screening biomarkers of a prognosis for PCa.

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Integrative analysis of the gastric cancer long non‑coding RNA‑associated competing endogenous RNA network

Cited by 7 publications

References 52 publications

Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial‐mesenchymal transition and angiogenesis

Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial‐mesenchymal transition and angiogenesis

Long noncoding RNA GK‐IT1 promotes esophageal squamous cell carcinoma by regulating MAPK1 phosphorylation

A DNA methylation profile of long non-coding RNAs can predict OS in prostate cancer

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