Integrative Control Between Proton Pumps and SOS1 Antiporters in Roots is Crucial for Maintaining Low Na+ Accumulation and Salt Tolerance in Ammonium-Supplied Sorghum bicolor

Abstract

An effective strategy for re-establishing K+ and Na+ homeostasis is a challenge for the improvement of plant performance in saline soil. Specifically, attempts to understand the mechanisms of Na+ extrusion from plant cells, the control of Na+ loading in the xylem and the partitioning of the accumulated Na+ between different plant organs are ongoing. Our goal was to provide insight into how an external nitrogen source affects Na+ accumulation in Sorghum bicolor under saline conditions. The NH4+ supply improved the salt tolerance of the plant by restricting Na+ accumulation and improving the K+/Na+ homeostasis in shoots, which was consistent with the high activity and expression of Na+/H+ antiporters and proton pumps in the plasma membrane and vacuoles in the roots, resulting in low Na+ loading in the xylem. Conversely, although NO3--grown plants had exclusion and sequestration mechanisms for Na+, these responses were not sufficient to reduce Na+ accumulation. In conclusion, NH4+ acts as an efficient signal to activate co-ordinately responses involved in the regulation of Na+ homeostasis in sorghum plants under salt stress, which leads to salt tolerance.

NaCl-PRIMING MITIGATES OXIDATIVE DAMAGE AND Na+ ACCUMULATION AND ENHANCES SALT TOLERANCE IN SORGHUM PLANTS

“…2A and 2B). Consistently, a low Na + accumulation together with elevated K + /Na + ratio has been considered as an important mechanism for salt tolerance in plant species (Yamaguchi et al, 2013;Miranda et al, 2017). In fact, our findings demonstrated that the most salt tolerant sorghum plants (P10-acclimated) showed a lower content of toxic Na + ions coupled to greater K + /Na + ratio in leaves ( Fig.…”

“…Salt stress is widely known to impair plant growth and productivity by disturbing numerous physiological and biochemical processes like ion homeostasis and ion activities (Gomes-Filho et al, 2008;Miranda et al, 2017). Concordantly, in this study, the dry mass of shoot (DMS), root (DMR) and total (DMT) was drastically decreased by salinity, irrespective of NaCl priming (Fig.…”

Analise Primaria De Transcriptoma De Tecido Testicular De Hamsters (Mesocricetus Auratus) Infectados Com Vírus Zika

“…Foi utilizado o método Deseq2 para análise diferencial de dados de contagem geradas no Featurescounts, com o objetivo de estimar a retração para dispersões e mudanças de dobras (LOVE, et al 2014). (MIRANDA et al, 2017;WDOWIKOWSKA;KLOBUS, 2016;OLFATMIRI et al,2014). Embora essas proteínas apresentem papéis fisiológicos importantes, estudos sobre a sua caracterização gênica e distribuição filogenética ainda são escassos, limitando-se a poucas espécies.…”

Introduction to Bioinformatics

“…ATPases de membrana plasmática são proteínas integrais que atuam no bombeamento de prótons do citoplasma para o ambiente extracelular utilizando a energia química da hidrolise do ATP gerando força próton motriz que é fundamental para a atividade de um grande grupo de transportadores secundários que movem íons e metabólitos contra gradiente de concentração (PALMGREN, 2001; WDOWIKOWSKA;KLOBUS, 2016). Em plantas, essas bombas de prótons estão relacionadas a diferentes Aeluropus littoralis demonstraram um aumento no perfil de expressão gênica dessas H + -ATPases quando submetidas a condições de estresse salino e hídrico(MIRANDA et al, 2017; WDOWIKOWSKA; KLOBUS, 2016;OLFATMIRI et al,2014). Embora essas proteínas apresentem papéis fisiológicos importantes, estudos sobre a sua caracterização gênica e distribuição filogenética ainda são escassos, limitando-se a poucas espécies.…”