Integrative description of bisexual Paramacrobiotus experimentalis sp. nov. (Macrobiotidae) from republic of Madagascar (Africa) with microbiome analysis

Kaczmarek, Łukasz; Roszkowska, Milena; Poprawa, Izabela; Janelt, Kamil; Kmita, Hanna; Gawlak, Magdalena; Fiałkowska, Edyta; Mioduchowska, Monika

doi:10.1016/j.ympev.2019.106730

Cited by 46 publications

(59 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies of the bacterial microbiota of glacier invertebrates are rare [ 8 – 10 ]; regrettably, the same is true for representatives of the phylum Tardigrada, one of the most cosmopolitan and ubiquitous invertebrates [ 29 , 53 , 54 ]. Investigations of limnoterrestrial tardigrade microbiomes showed they were species-specific, but still moderated by the environment [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Trophic and symbiotic links between obligate-glacier water bears (Tardigrada) and cryoconite microorganisms

et al. 2022

View full text Add to dashboard Cite

Insights into biodiversity and trophic webs are important for understanding ecosystem functions. Although the surfaces of glaciers are one of the most productive and biologically diverse parts of the cryosphere, the links between top consumers, their diet and microbial communities are poorly understood. In this study, for the first time we investigated the relationships between bacteria, fungi and other microeukaryotes as they relate to tardigrades, microscopic metazoans that are top consumers in cryoconite, a biologically rich and productive biogenic sediment found on glacier surfaces. Using metabarcoding (16S rDNA for bacteria, ITS1 for fungi, and 18S rDNA for other microeukaryotes), we analyzed the microbial community structures of cryoconite and compared them with the community found in both fully fed and starved tardigrades. The community structure of each microbial group (bacteria, fungi, microeukaryotes) were similar within each host group (cryoconite, fully fed tardigrades and starved tardigrades), and differed significantly between groups, as indicated by redundancy analyses. The relative number of operational taxonomic units (ZOTUs, OTUs) and the Shannon index differed significantly between cryoconite and tardigrades. Species indicator analysis highlighted a group of microbial taxa typical of both fully fed and starved tardigrades (potential commensals), like the bacteria of the genera Staphylococcus and Stenotrophomonas, as well as a group of taxa typical of both cryoconite and fully fed tardigrades (likely part of the tardigrade diet; bacteria Flavobacterium sp., fungi Preussia sp., algae Trebouxiophyceae sp.). Tardigrades are consumers of bacteria, fungi and other microeukaryotes in cryoconite and, being hosts for diverse microbes, their presence can enrich the microbiome of glaciers.

show abstract

Section: Discussionmentioning

confidence: 99%

Trophic and symbiotic links between obligate-glacier water bears (Tardigrada) and cryoconite microorganisms

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In total, four molecular markers were amplified: one mitochondrial gene, i.e., COIthe cytochrome oxidase subunit I; three nuclear markers, i.e., 18S rRNA-the small ribosome subunit and 28S rRNA-the large ribosome subunit as well as ITS-2-the internal transcribed spacer-2. The polymerase chain reaction (PCR) amplification was performed according to Kaczmarek et al [44]. The sequences of primers applied to amplify molecular markers are listed in Table 1.…”

Section: Genotypingmentioning

confidence: 99%

Integrative Descriptions of Two New Tardigrade Species along with the New Record of Mesobiotus skorackii Kaczmarek et al., 2018 from Canada

et al. 2021

Self Cite

View full text Add to dashboard Cite

Two new tardigrade species from a moss sample collected in Canada, one representing Macrobiotus hufelandi complex and the second one belonging to the genus Bryodelphax, are described. Integrative analysis was undertaken based on morphological and morphometric data (using both light and scanning electron microscopy (SEM)) combined with multilocus molecular analysis (nuclear sequences, i.e., 18S rRNA, 28S rRNA and ITS-2 as well as mitochondrial COI barcode sequences). Based on COI sequences, Macrobiotus birendrai sp. nov. is most similar to Mac. canaricus (p-distance 17%), whereas Bryodelphax mareki sp. nov. is most similar to Bry. parvulus (p-distance 16%). Both species differ also from their congeners in some morphological and morphometric characters of adults and/or details of egg chorion. Additionally, a large population of Mesobiotus skorackii was found in the sample and this is the first report of this species outside its terra typica in Kirghizia. The original description of this species was prepared based solely on the morphology and morphometry, therefore, here we provide updated data for this species enclosing morphometric and molecular data for the Canadian population.

show abstract

“…Coupled with strong negative contributions of Fungi, Amoebozoa, and Acari, these data may suggest interspecies interaction preferences of M. shonaicus. These data provide an ecosystem-wide perspective on tardigrade inter-specific association, which could complement more detailed analyses on tardigrade symbiosis [21,22].…”

Section: Discussionmentioning

confidence: 90%

“…This microenvironment harbors the substrate moss plant, a variety of meiofauna including tardigrades, nematodes, arthropods, and rotifers, and communities of fungi, protozoans, and bacteria. To study such a complex ecosystem and to elucidate the ecology surrounding tardigrades as recently being explored [21,22], I employed an amplicon sequencing approach using a set of universal primers for both microbes and eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Metabarcoding of Eukaryotes and Prokaryotes to Elucidate the Community Structures within Tardigrade Microhabitats

Arakawa

2020

Diversity

View full text Add to dashboard Cite

Tardigrades are microscopic invertebrates that can withstand complete desiccation, but their interspecies interactions with prokaryotes and eukaryotes within their microhabitat remain relatively unexplored. Here, I utilized combined metabarcoding of eukaryotes and prokaryotes to simultaneously identify entire community structures within xeric and mesic mosses that harbor tardigrades. The populations of organisms within the microecosystems were successfully determined in 45 xeric moss samples and 47 mesic moss samples. Organismal composition was largely consistent regardless of the moss/lichen substrate, but significantly varied in the two tested locations, possibly because of the differences in environmental humidity. Xeric mosses containing xerophilic tardigrades and other anhydrobiotic invertebrates tended to have significantly limited biological diversity and prokaryotic population dominated by cyanobacteria, suggesting a selection due to extreme desiccation. A combined metabarcoding approach to identify both eukaryotes and prokaryotes can successfully elucidate community structures within microscopic ecosystems, and this can be a potential approach to study the microecology of meiofauna, including tardigrades.

show abstract

Integrative description of bisexual Paramacrobiotus experimentalis sp. nov. (Macrobiotidae) from republic of Madagascar (Africa) with microbiome analysis

Cited by 46 publications

References 131 publications

Trophic and symbiotic links between obligate-glacier water bears (Tardigrada) and cryoconite microorganisms

Trophic and symbiotic links between obligate-glacier water bears (Tardigrada) and cryoconite microorganisms

Integrative Descriptions of Two New Tardigrade Species along with the New Record of Mesobiotus skorackii Kaczmarek et al., 2018 from Canada

Simultaneous Metabarcoding of Eukaryotes and Prokaryotes to Elucidate the Community Structures within Tardigrade Microhabitats

Contact Info

Product

Resources

About