Integrative microbial and transcriptomic analysis reveals the lignocellulosic biomass degrading mechanism by bamboo snout beetle

Tang, Hao; Li, Yuanqiu; Wang, Mingjun; Luo, Chaobing

doi:10.1016/j.indcrop.2023.117194

Cited by 2 publications

(1 citation statement)

References 59 publications

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“…Correlation analysis in our study reveals a significant positive correlation between Lactococcus , Weissella , Pseudomonas and the digestibility of cellulose and hemicellulose, suggesting that these bacterial phyla enhance the cellulose digestion ability of the host. Our study also finds a high proportion of Weissella in L. migratoria gut microbiota, consistent with the report of Tang et al ( 66 ) wherein Weissella is strongly associated with swarming locusts. Moreover, Wada-Katsumata ( 67 ) showed that Weissella is widespread in swarming cockroaches, where it causes aggregation of German cockroaches, further implying that Weissella contributes to swarm formation and insect adaptation to their environment.…”

Section: Discussionsupporting

confidence: 93%

Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility

Li,

Liang

et al. 2024

mSystems

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Locusta migratoria is an important phytophagous pest, and its gut microbial communities play an important role in cellulose degradation. In this study, the gut microbial and cellulose digestibility dynamics of Locusta migratoria were jointly analyzed using high-throughput sequencing and anthrone colorimetry. The results showed that the gut microbial diversity and cellulose digestibility across life stages were dynamically changing. The species richness of gut bacteria was significantly higher in eggs than in larvae and imago, the species richness and cellulose digestibility of gut bacteria were significantly higher in early larvae (first and second instars) than in late larvae (third to fifth instars), and the diversity of gut bacteria and cellulose digestibility were significantly higher in imago than in late larvae. There is a correlation between the dynamics of gut bacterial communities and cellulose digestibility. Enterobacter , Lactococcus, and Pseudomonas are the most abundant genera throughout all life stages. Six strains of highly efficient cellulolytic bacteria were screened, which were dominant gut bacteria. Carboxymethyl cellulase activity (CMCA) and filter paper activity (FPA) experiments revealed that Pseudomonas had the highest cellulase enzyme activity. This study provides a new way for the screening of cellulolytic bacteria and lays the foundation for developing insects with significant biomass into cellulose-degrading bioreactors. IMPORTANCE Cellulose is the most abundant and cheapest renewable resource in nature, but its degradation is difficult, so finding efficient cellulose degradation methods is an urgent challenge. Locusta migratoria is a large group of agricultural pests, and the large number of microorganisms that inhabit their intestinal tracts play an important role in cellulose degradation. We analyzed the dynamics of Locusta migratoria gut microbial communities and cellulose digestibility using a combination of high-throughput sequencing technology and anthrone colorimetry. The results revealed that the gut microbial diversity and cellulose digestibility were dynamically changed at different life stages. In addition, we explored the intestinal bacterial community of Locusta migratoria across life stages and its correlation with cellulose digestibility. The dominant bacterial genera at different life stages of Locusta migratoria were uncovered and their carboxymethyl cellulase activity (CMCA) and filter paper activity (FPA) were determined. This study provides a new avenue for screening cellulolytic bacteria and lays the foundation for developing insects with significant biomass into cellulose-degrading bioreactors.

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Section: Discussionsupporting

confidence: 93%

Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility

Li,

Liang

et al. 2024

mSystems

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Saccharification and co-fermentation of lignocellulosic biomass by a cockroach-gut bacterial symbiont and yeast cocktail for bioethanol production

Echa,

Ekpenyong,

Edeghor

et al. 2024

BMC Biotechnol

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Background The eco-friendly transformation of agro-industrial wastes through microbial bioconversion could address sustainability challenges in line with the United Nations’ Sustainable Development Goals. The bulk of agro-industrial waste consists of lignocellulosic materials with fermentable sugars, predominantly cellulose and hemicellulose. A number of pretreatment options have been employed for material saccharification toward successful fermentation into second-generation bioethanol. Biological and/or enzymatic pretreatment of lignocellulosic waste substrates offers eco-friendly and sustainable second-generation bioethanol production opportunities that may also contribute to waste management without affecting food security. In this study, we isolated a promising filamentous bacterium from the guts of cockroaches with commendable cellulolytic activity. The matrices of sequential statistics, from one-factor-at-a-time (OFAT) through significant variable screening by Placket-Burman design (PBD) to Box‒Behnken design of a surface methodology (BBD-RSM), were employed for major medium variable modeling and optimization by solid-state fermentation. The optimized solutions were used to saccharify lignocellulose in real time, and the kinetics of reducing sugar accumulation were subsequently evaluated to determine the maximum concentration of sugars extracted from the lignocellulose. The hydrolysate with the highest reducing sugar concentration was subjected to fermentation by Saccharomyces cerevisiae , Klyuveromyces marxianus and a mixture of both, after which the ethanol yield, concentration and fermentation efficiency were determined. Results Sequential statistics revealed that rice husk powder, corn cob powder, peptone and inoculum volume were significant variables for the bioprocess at 59.8% (w/w) rice husk powder, 17.8% (w/w) corn cob powder, 38.8% (v/w; 10 9 cfu/mL) inoculum volume, and 5.3% (w/w) peptone. These conditions mediated maximum cellulolytic and xylanolytic activities of 219.93 ± 18.64 FPU/mL and 333.44 ± 22.74 U/mL, respectively. The kinetics of saccharification of the lignocellulosic waste under optimized conditions revealed two peaks of reducing sugar accumulation between 16 and 32 h and another between 56 and 64 h. Conclusions Although K. marxianus had a significantly greater fermentation efficiency than S. cerevisiae , fermentation with a 50:50 (% v/v) mixture of both yeasts led to 88.32% fermentation efficiency with 55.56 ± 0.19 g/L crude bioethanol, suggesting that inexpensive, eco-friendly and sustainable bioethanol production could be obtained from renewable energy sources. Supplementary Information The online version contains supplementary material available at 10.1186/s12896-024-00932-8.

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Integrative microbial and transcriptomic analysis reveals the lignocellulosic biomass degrading mechanism by bamboo snout beetle

Cited by 2 publications

References 59 publications

Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility

Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility

Saccharification and co-fermentation of lignocellulosic biomass by a cockroach-gut bacterial symbiont and yeast cocktail for bioethanol production

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