Integrative microRNA–mRNA Analysis of Muscle Tissues in Qianhua Mutton Merino and Small Tail Han Sheep Reveals Key Roles for oar-miR-655-3p and oar-miR-381-5p

Lu, Songyan; Bai, Man; Xiang, Lujie; Li, Jiarong; Jia, Chao; Huai-zhi, Jiang

doi:10.1089/dna.2018.4408

Cited by 22 publications

(20 citation statements)

References 65 publications

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“…Thus, miRNAs such as miR-23a, miR-199b, miR-381, and so on were expressed specifically in the juvenile stage; miR-1277, miR-1307, miR-2334, and so on were expressed specifically in the adolescent stage; miR-10b, miR-191, and miR-362-3p were expressed specifically in the adult stage; and miR-27a-3p, miR-29b, miR-30d, and so on were expressed specifically in the aged stage. These development stage-specific miRNAs were expressed at very low levels, but some, such as miR-199b, miR-23a, miR-381, and miR-27a-3p, were closely related to growth and development [23,[35][36][37]; miR-191 and miR-29b were closely related to aging [38,39]. This result indicates that miRNAs with development-stage specificity might play an important role in the muscle development and aging of sika deer.…”

Section: Discussionmentioning

confidence: 88%

“…Thus, changes in the expression of interrelated miRNAs and target genes play a decisive role in controlling age-related alterations in muscle mass and function. In recent years, Illumina sequencing technology has been increasingly used to identify potential key interactions between miRNAs and mRNAs in the development of skeletal muscle [23][24][25]. However, miRNA-mRNA regulatory networks associated with the muscle growth, development and aging of sika deer have not been studied.…”

Section: Introductionmentioning

confidence: 99%

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Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Jia

Liu

et al. 2020

Genes

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Studies of the gene and miRNA expression profiles associated with the postnatal late growth, development, and aging of skeletal muscle are lacking in sika deer. To understand the molecular mechanisms of the growth and development of sika deer skeletal muscle, we used de novo RNA sequencing (RNA-seq) and microRNA sequencing (miRNA-seq) analyses to determine the differentially expressed (DE) unigenes and miRNAs from skeletal muscle tissues at 1, 3, 5, and 10 years in sika deer. A total of 51,716 unigenes, 171 known miRNAs, and 60 novel miRNAs were identified based on four mRNA and small RNA libraries. A total of 2,044 unigenes and 11 miRNAs were differentially expressed between adolescence and juvenile sika deer, 1,946 unigenes and 4 miRNAs were differentially expressed between adult and adolescent sika deer, and 2,209 unigenes and 1 miRNAs were differentially expressed between aged and adult sika deer. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DE unigenes and miRNA were mainly related to energy and substance metabolism, processes that are closely associate with the growth, development, and aging of skeletal muscle. We also constructed mRNA–mRNA and miRNA–mRNA interaction networks related to the growth, development, and aging of skeletal muscle. The results show that mRNA (Myh1, Myh2, Myh7, ACTN3, etc.) and miRNAs (miR-133a, miR-133c, miR-192, miR-151-3p, etc.) may play important roles in muscle growth and development, and mRNA (WWP1, DEK, UCP3, FUS, etc.) and miRNAs (miR-17-5p, miR-378b, miR-199a-5p, miR-7, etc.) may have key roles in muscle aging. In this study, we determined the dynamic miRNA and unigenes transcriptome in muscle tissue for the first time in sika deer. The age-dependent miRNAs and unigenes identified will offer insights into the molecular mechanism underlying muscle development, growth, and maintenance and will also provide valuable information for sika deer genetic breeding.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Jia

Liu

et al. 2020

Genes

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“…Crossbreeding local sheep breeds with specialized breeds is the most common method used to rapidly improve the growth rate, meat quality, and production performance of a local sheep breed [4]. This crossbreeding method has been utilized to develop some excellent local breeds, such as Qianhua Mutton Merino (QHMM) sheep [5] and large-tailed Han (LTH) sheep [6]. Understanding the mechanisms of crossbreeding from a genetic perspective is important for optimizing sheep production performance and meat quality traits.…”

Section: Introductionmentioning

confidence: 99%

“…The association between phenotypes and genotypes in many livestock species is the result of precisely orchestrated molecular regulation networks [15], especially those markers associated with muscle growth, development, and muscle myofibers (myosin, troponin, myoglobin, and tropomyosin) [5,9],which are the dominant factors determining livestock production performance and meat quantity. In the present study, MG sheep were crossbred with STH sheep.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Identifying the Different Molecular Genetic Markers Related to Production Performance and Meat Quality in Longissimus Dorsi Tissues of MG × STH and STH Sheep

ShuRu

Zhang

et al. 2020

Genes

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Crossbred sheep have many prominent traits, such as excellent production performance and high-quality meat, when compared to local sheep breeds. However, the genetic molecular markers related to these characteristics remain unclear. The crossbred MG × STH (small-tailed Han sheep (STH) × Mongolian sheep (MG)) breed and the STH breed were selected to measure production performance and meat quality. We used 14 indexes of production performance and meat quality, which in the MG × STH population showed significant differences compared to the STH breed. Subsequently, the longissimus dorsi from the two sheep were subjected to comparative transcriptomic analyses to identify differentially expressed genes (DEGs) related to production performance and meat quality. A total of 874 DEGs were identified between the two sheep groups. A total of 110 unique DEGs related to sheep production performance and meat quality were selected as the candidate DEGs. We found 6 production-performance-related and 30 meat-quality-related DEGs through a correlation analysis, including SPARC, ACVRL1, FNDC5, and FREM1. The expression levels of 11 DEGs were validated by real-time PCR, and the results were in accordance with the results of the comparative transcriptomic and correlation analyses. These results will assist in understanding sheep heterosis and molecular marker-assisted selection.

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“…1 High-quality domesticated sheep breed is a valuable genetic resource for the global animal husbandry. 2 The fat-tailed sheep is widely bred in China and other countries, due to its high adaptability to different climatic conditions, disease resistance and high production in poor nutritional situations. 3 Among, the fat deposition in the tails plays a crucial role for the sheep to adapt to nutritionally challenging environments.…”

mentioning

confidence: 99%

Expression Patterns of SREBF1 and SREBF2 in Liver and Adipose Tissues of Two Fat-Tailed Sheep Breeds

Chen¹,

Qiao²,

Han³

et al. 2020

Preprint

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Background The fat-tailed sheep is raised largely in China and other countries due to its high adaptability to nutritionally challenging environments and disease resistance as well as fat deposition in tails. So the purpose of the study is to explore the expression pattern and regulation mechanism of sterol regulatory element binding proteins (SREBPs) in liver and adipose tissues of fat-tailed sheep bases on its role in fat metabolism and deposition with sheep grown and development. Methods Two representative Chinese fat-tailed sheep breeds i.e. Guangling Large Tailed (GLT) and Small Tailed Han (STH) were employed to evaluate the ontogenetic expressions of SREBF1 and SREBF2 from seven different adipose tissues and liver at 4, 6, 8, 10 and 12 month by real-time PCR. Five serum indicators were detected, and the function speculation of SREBP1 and SREBP2 were evaluated by bioinformatics approaches. Results Serum indicators, especially total cholesterol TC and non-esterified fatty acid (NEFA) showed obvious differences and dynamical changes with the different development ages, gender factors only had a significant effect on serum NEFA concentrations. Both SREBF1 and SREBF2 mRNA expression in sheep were significantly affected by breed, tissue, age factors, and show a significant positive correlation (r = 0.286). Merely the expression of SREBF1 gene in STH is significantly higher than that in GLT, but SREBF2 gene expression is opposite. The expressions of SREBF1/2 in liver are extremely higher than that in seven adipose tissues, the interaction of two factors between breed and month age, breed and tissue, and tissue and gender also significantly affects its expression. Subcellular locations and function prediction imply that SREBP1/2 expressions are closely related with metabolism processes in cells. Conclusions The mRNA expression profiling of SREBF1 and SREBF2 showing a breed-specific, gender-specific, and temporal and spatial expressions differences, which imply that SREBF1/2 play a crucial role in lipid metabolism regulation during growth and development of two fat-tailed sheep. This study provides a phenotypic basis for further revealing the genetic mechanism in lipid metabolism and fat deposition that causes differences in ovine tail types, which also provides a novel insight for improving quality of meat.

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Integrative microRNA–mRNA Analysis of Muscle Tissues in Qianhua Mutton Merino and Small Tail Han Sheep Reveals Key Roles for oar-miR-655-3p and oar-miR-381-5p

Cited by 22 publications

References 65 publications

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Comparative Transcriptome Analysis Identifying the Different Molecular Genetic Markers Related to Production Performance and Meat Quality in Longissimus Dorsi Tissues of MG × STH and STH Sheep

Expression Patterns of SREBF1 and SREBF2 in Liver and Adipose Tissues of Two Fat-Tailed Sheep Breeds

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