Integrative taxonomy of Anisakidae and Raphidascarididae (Nematoda) in Paralichthys patagonicus and Xystreurys rasile (Pisces: Teleostei) from Brazil

Fonseca, Michelle Cristie Gonçalves da; Knoff, Marcelo; Felizardo, Nilza Nunes; Azevedo, Maria Isabel Nogueira Di; Torres, Eduardo José Lopes; Gomes, Delir Corrêa; Iñiguez, Alena Mayo; Clemente, Sérgio Carmona de São

doi:10.1016/j.ijfoodmicro.2016.07.026

Cited by 27 publications

(27 citation statements)

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“…The specimens of H. deardorffoverstreetorum (L 3 ) found in L. villarii exhibited similar morphological and morphometric features to the specimens from P. isosceles, P. patagonicus, X. rasile, C. guatucupa, Chaetodipterus faber (Broussonet, 1782), Trachinotus carolinus (Linnaeus, 1766), and Priacanthus arenatus Cuvier, 1829, all hosts collected from the state of Rio de Janeiro FONTENELLE et al 2013;RIBEIRO et al 2014;KURAIEM et al 2016;FONSECA et al 2016).…”

Section: Resultsmentioning

confidence: 74%

“…The nematode larvae found dead were fixed in AFA (70% alcoholformaldehyde -acetic acid), while live larvae were fixed in hot AFA at 60 ºC, so they would die distended, preserved in a solution of 70 °GL ethanol plus 5% glycerin and clarified with Amman's lactophenol . Taxonomic classification of nematodes was in accordance with PETTER and MAILLARD (1988);TIMI et al (2001); FELIZARDO et al (2009);FONSECA et al (2016).…”

Section: Methodsmentioning

confidence: 71%

“…from Genypterus brasiliensis Regan, 1903; A. simplex from P. isosceles (FELIZARDO et al, 2009) and from Lophius gastrophysus Miranda- Ribeiro, 1915(SAAD et al, 2012; Anisakis sp. from C. guatucupa (FONTENELLE et al, 2013); and A. typica from P. patagonicus (FONSECA et al, 2016). Its indices were slightly lower than those for Anisakis type I from L. gastrophysus (SAAD et al, 2012); Anisakis sp.…”

Section: Discussionmentioning

confidence: 92%

“…When compared to the specimens of other reports, one morphological difference was observed; the length of the intestinal cecum is approximately half the length of the ventriculus, while those Terranova sp. specimens from Engraulis anchiota Hubbs & Marini, 1935, from Argentina and Uruguay (TIMI et al, 2001) and Paralichthys isosceles Jordan, 1890, P. patagonicus Jordan, 1889, Xystreurys rasile (Jordan, 1891) and Cynoscion guatucupa (Cuvier, 1830) from state of Rio de Janeiro, Brazil, had an intestinal cecum that was always almost twice the length of the ventriculus (FELIZARDO et al, 2009;FONTENELLE et al, 2013;FONSECA et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…The nematodes of the families Anisakidae and Raphidascarididae have been reported parasitizing several Brazilian marine fish in studies on classical and molecular taxonomy, ecology and hygiene and health, since the larvae migrate after the death of the hosts (FELIZARDO et al, 2009;DIAS et al, 2010DIAS et al, ,2011KNOFF et al, ,2013FONTENELLE et al, 2013FONTENELLE et al, ,2015RIBEIRO et al, 2014;PANTOJA et al, 2015;KURAIEM et al, 2016;FONSECA et al, 2016). Human infections with these nematodes have been reported in Europe (Netherlands, Spain, France, Italy, Belgium, Denmark, England and Germany), in the Americas (United States of America, Chile, Peru and Brazil), in Korea and, especially, in Japan, where more than 2,000 cases occur annually (ADAMS et al, 1997;AUDICANA et al, 2002;ACHA and SZYFRES, 2003;CHAI et al, 2005;CRUZ et al, 2010;D'AMICO et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Larvas de nematoides e cestoides de importí¢ncia higiênico-sanitária em Lopholatilus villarii (Actinopterygii) no Estado do Rio de Janeiro, Brasil

et al. 2017

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LARVAS DE NEMATOIDES E CESTOIDES DE IMPORTÂNCIA HIGIÊNICO-SANITÁRIA EM Lopholatilus villarii (ACTINOPTERYGII) NO ESTADO DO RIO DE JANEIRO, BRASILPalavras-chave: Malacanthidae; Anisakidae; Raphidascarididae; Trypanorhyncha. ABSTRACTThe tile fish, Lopholatilus villarii, occurs from Brazil to Argentina where it is commercially important it because possesses highly appreciated meat. The aim of this study was to identify the larvae of anisakid and raphidascaridid nematodes and trypanorhynch cestodes in L. villarii purchased in fish markets of the municipality of Niterói, state of Rio de Janeiro, Brazil, and calculate their parasitological indices and present their sites of infection. From August 2015 to September 2016, 31 specimens of L. villarii were investigated. The fish were necropsied and their viscera and musculature analyzed. The helminths found were processed according to standard helminthological techniques for taxonomic identification. Of 31 fish analyzed, 28 (90.3%) were parasitized with larvae of anisakid and raphidascaridid nematodes, Anisakis sp., Terranova sp., Pseudoterranova sp. and Hysterothylacium deardorffoverstreetorum (L 3 and L 4 ) and plerocercus of the trypanorhynch cestode Otobothrium sp. Their parasitological indices were calculated. The helminths parasitized mainly the serosas of intestine, stomach and liver, and H. deardorffoverstreetorum was also parasitizing the abdominal musculature of one fish. This is the first report of anisakid and raphidascaridid nematodes and trypanorhynch cestodes in L. villarii. Hygienic-sanitary aspects were discussed.

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Section: Resultsmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Larvas de nematoides e cestoides de importí¢ncia higiênico-sanitária em Lopholatilus villarii (Actinopterygii) no Estado do Rio de Janeiro, Brasil

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Morphology and molecular characterization hold hands: clarifying the taxonomy of Hysterothylacium (Nematoda: Anisakidae) larval forms

et al. 2016

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During the last decade, many morphotypes of larval forms of Hysterothylacium have been proposed; however, their boundaries are taxonomically unclear. We compare newly collected material with data available for Hysterothylacium larvae, based on genetics and morphology, in order to evaluate their relationships and simplify their classification. The previously described Hysterothylacium larval type IV, type V, and type X were found in several marine fishes from Brazil. A new Hysterothylacium larval type XV, parasitic in Elops saurus from State of Sergipe, north-eastern Brazil, was proposed based on its genetic singularity and the presence of numerous spines throughout the body. Based upon phylogenetic reconstruction using the ribosomal DNA (rDNA) ITS2 sequences along with morphological traits, the following third stage larvae were considered valid (synonyms are inside parenthesis) type III (= types VII and VIII), type V (= types VI, XII, and XIII), and type X (= type XIV). Despite the lack of molecular data, Hysterothylacium larval type II is considered as synonym of type I because of their identical morphology. The morphometry of Hysterothylacium third stage larvae proved to be weak as a diagnostic tool due to its high rates of variation. Phylogenetic reconstruction using the concatenated sequences of rDNA ITS1-5.8S-ITS2 and the mitochondrion DNA (mtDNA) cox2 revealed that the sampled Hysterothylacium larval types V may represent three different species and H. deardorffovestreetorum may represent two different taxa. Furthermore, H. deardorffovestreetorum is considered species inquirenda due to its problematic description and diagnosis which are based only on larvae. A key to Hysterothylacium larvae from marine fishes is provided.

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Integrative taxonomy of anisakid nematodes in stranded cetaceans from Brazilian waters: an update on parasite’s hosts and geographical records

2017

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Anisakidae are represented mainly by species of Anisakis Dujardin, 1845, Pseudoterranova Krabbe, 1878, and Contracaecum Railliet and Henry, 1913. Integrative taxonomy, based on morphological and genetic approaches, can provide a precise identification of species, increasing the knowledge of their systematics, biology, and evolution. In this study, integrative taxonomy was applied in order to identify, at generic and/or specific taxonomic level, 245 anisakids from 63 cetaceans of 12 different species, which stranded in the Brazilian coast. Parasites from nine hosts were preserved by freezing and, from 54 hosts, were fixed in 70% ethanol (n = 43) or alcohol-formalin-acetic (n = 11) for a period varying from 6 to 19 years. The morphological analysis allowed the identification of 171 specimens at genus level: 120 as Anisakis sp. clade I, 47 as Anisakis sp. clade II, and four as Pseudoterranova sp. From those 15, specimens were identified at species level, seven as Anisakis typica, seven as Anisakis paggiae and one as Anisakis brevispiculata. Moreover, 74 specimens were identified as belonging to Anisakidae family. The genetic analysis based on cox2 gene allowed the identification/confirmation of Anisakis typica (n = 33), Anisakis paggiae (n = 1), and for the first time in the Brazilian coast, Anisakis brevispiculata (n = 4) and Anisakis ziphidarum (n = 1). Additionally, a probably new Anisakis species, genetically close to A. paggiae, is reported infecting a dwarf sperm whale. The results clearly indicated that the preservation of nematodes influenced significantly on the level of taxonomic definition, in both morphological and, mainly, genetic analyses. Pseudoterranova sp. was identified for the first time in the spinner dolphin, Stenella longirostris. The detection of nine A. paggiae specimens, in three Kogia breviceps animals, substantiates the austral occurrence of this species. The identification of A. brevispiculata in Kogia sima and K. breviceps confirms Kogiidae whales as their most common hosts. Anisakis typica was corroborated as a generalist species, the most widely distributed in the Brazilian coast, infecting a broad variety of Delphinidae and Kogiidae, with two new geographical records in Stenella frontalis and Lagenodelphis hosei. The study showed a new scenery of parasite diversity of marine anisakids, updating the Anisakis species distribution.

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Integrative taxonomy of Anisakidae and Raphidascarididae (Nematoda) in Paralichthys patagonicus and Xystreurys rasile (Pisces: Teleostei) from Brazil

Cited by 27 publications

References 37 publications

Larvas de nematoides e cestoides de importí¢ncia higiênico-sanitária em Lopholatilus villarii (Actinopterygii) no Estado do Rio de Janeiro, Brasil

Larvas de nematoides e cestoides de importí¢ncia higiênico-sanitária em Lopholatilus villarii (Actinopterygii) no Estado do Rio de Janeiro, Brasil

Morphology and molecular characterization hold hands: clarifying the taxonomy of Hysterothylacium (Nematoda: Anisakidae) larval forms

Integrative taxonomy of anisakid nematodes in stranded cetaceans from Brazilian waters: an update on parasite’s hosts and geographical records

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Integrative taxonomy of Anisakidae and Raphidascarididae (Nematoda) in Paralichthys patagonicus and Xystreurys rasile (Pisces: Teleostei) from Brazil

Cited by 27 publications

References 37 publications

Larvas de nematoides e cestoides de importí­¢ncia higiênico-sanitária em Lopholatilus villarii (Actinopterygii) no Estado do Rio de Janeiro, Brasil

Larvas de nematoides e cestoides de importí­¢ncia higiênico-sanitária em Lopholatilus villarii (Actinopterygii) no Estado do Rio de Janeiro, Brasil

Morphology and molecular characterization hold hands: clarifying the taxonomy of Hysterothylacium (Nematoda: Anisakidae) larval forms

Integrative taxonomy of anisakid nematodes in stranded cetaceans from Brazilian waters: an update on parasite’s hosts and geographical records

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Larvas de nematoides e cestoides de importí¢ncia higiênico-sanitária em Lopholatilus villarii (Actinopterygii) no Estado do Rio de Janeiro, Brasil

Larvas de nematoides e cestoides de importí¢ncia higiênico-sanitária em Lopholatilus villarii (Actinopterygii) no Estado do Rio de Janeiro, Brasil