Integrative taxonomy ofPeniculus,Metapeniculus, andTrifur(Siphonostomatoida: Pennellidae), copepod parasites of marine fishes from Chile: species delimitation analyses using DNA barcoding and morphological evidence

Romero, Raúl Castro; Montes, Martin Miguel; Martorelli, Sergio Roberto; Sepúlveda, Diego; Tapia, Santiago; Martínez-Aquino, Andrés

doi:10.1080/14772000.2016.1158213

Cited by 20 publications

(11 citation statements)

References 64 publications

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“…We confirmed that this parasite from black sea bass is in fact a larval and adult form of L. radiatus prior to female metamorphosis. Linking the developmental stages in the black sea bass gill to L. radiatus was confirmed in the present investigation by sequencing the COI gene, which is the main target gene for barcoding of invertebrates (Folmer et al ., 1994; Raupach et al ., 2015; Castro-Romero et al ., 2016). This finding suggests that the species L. centropristi should be abolished, as these are in fact stages of L. radiatus .…”

Section: Discussionmentioning

confidence: 99%

“…The identities within the 28S rDNA sequences supported a similar pattern. The COI gene had the most representation for pennellid copepods because this is the target gene for barcoding of invertebrates (Folmer et al ., 1994; Raupach et al ., 2015) and it has been shown to be a useful marker for taxonomy within Pennellidae (Castro-Romero et al ., 2016). Because of high nucleotide divergence rates between species, we compared translated protein sequences, since amino acids are more highly conserved than nucleotides in evolution.…”

Section: Discussionmentioning

confidence: 99%

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Black sea bass are a host in the developmental cycle of Lernaeenicus radiatus (Copepoda: Pennellidae): insights into parasite morphology, gill pathology and genetics

Lovy¹,

Friend²

2019

Parasitology

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Lernaeenicus radiatus, a mesoparasitic pennellid copepod, has long been known in the northwest Atlantic with metamorphosed females infecting the muscle of marine fish. The study herein is the first to identify a definitive first host, black sea bass Centropristis striata, for L. radiatus supporting larval development to adults and sexual reproduction in the gills. This finding suggests a two-host life cycle for L. radiatus, with black sea bass as the first host. Heavy infections in the gill were associated with considerable pathology related to a unique and invasive attachment process that penetrated the gill and selectively attached to the gill filament cartilage. The morphology of the developing copepod was highly conserved with that of a related pennellid copepod, Lernaeocera branchialis, though was distinguished by the attachment process, unique pigmentation and other morphologic features described herein. Sequencing the small and large subunits of the ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes demonstrated L. radiatus to share closer identities with Lernaeocera and Haemobaphes spp. pennellid copepods rather than other Lernaeenicus spp. available in GenBank to date. Taxonomy of L. radiatus is discussed in relation to life cycles, tissue tropism, morphology and genetics of other closely related pennellid copepods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Black sea bass are a host in the developmental cycle of Lernaeenicus radiatus (Copepoda: Pennellidae): insights into parasite morphology, gill pathology and genetics

Lovy¹,

Friend²

2019

Parasitology

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“…Following recent studies (Castro-Romero et al, 2016;Montagna et al, 2016;Song et al, 2017), outgroup species were included in the species delimitation analyses for two main reasons. First, although related species tend to be unduly lumped by the GMYC model when distantly related species are included (https://francoismichonneau.net/gmyc-tutorial/), this was clearly not the case here, as more species were recognized with the GMYC criterion than in the two other analyses (Fig.…”

Section: Researchmentioning

confidence: 99%

Quantification of complex modular architecture in plants

et al. 2018

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Morphometrics, the assignment of quantities to biological shapes, is a powerful tool to address taxonomic, evolutionary, functional and developmental questions. We propose a novel method for shape quantification of complex modular architecture in thalloid plants, whose extremely reduced morphologies, combined with the lack of a formal framework for thallus description, have long rendered taxonomic and evolutionary studies extremely challenging. Using graph theory, thalli are described as hierarchical series of nodes and edges, allowing for accurate, homologous and repeatable measurements of widths, lengths and angles. The computer program MorphoSnake was developed to extract the skeleton and contours of a thallus and automatically acquire, at each level of organization, width, length, angle and sinuosity measurements. Through the quantification of leaf architecture in Hymenophyllum ferns (Polypodiopsida) and a fully worked example of integrative taxonomy in the taxonomically challenging thalloid liverwort genus Riccardia, we show that MorphoSnake is applicable to all ramified plants. This new possibility of acquiring large numbers of quantitative traits in plants with complex modular architectures opens new perspectives of applications, from the development of rapid species identification tools to evolutionary analyses of adaptive plasticity.

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“…Several works have discussed the importance of integrative taxonomy for meiofauna, with reciprocal illumination based on genetic and morphological identification (Castro-Romero et al 2016;Garraffoni et al 2019). In the past, specimens have typically been destroyed to extract DNA for molecular analyses and morphological data is thus obtained from different specimens (Di Capua et al 2017;Karanovic et al 2015).…”

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confidence: 99%

Non-destructive genome skimming for aquatic copepods

Vakati

Dodsworth

2020

Conservation Genet Resour

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Copepods are important ecologically and represent a large amount of aquatic biomass in both freshwater and marine systems. Despite this, the taxonomy of copepods and other meiofauna is not well understood, hampered by tiny sizes, cryptic taxa, intraspecific polymorphisms and total specimen destruction where DNA methods are employed. In this article we highlight these issues and propose a more up-to-date approach for dealing with them. Namely, we recommend non-destructive DNA extraction methods, coupled with high-throughput sequencing (HTS). Whilst DNA yields may be low, they should still be sufficient for HTS library preparation and DNA sequencing. At the same time morphological specimens can be preserved and the crucial link between morphology and DNA sequence is maintained. This is critical for an integrative taxonomy and a fuller understanding of biodiversity patterns as well as evolutionary processes in meiofauna.

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Integrative taxonomy ofPeniculus,Metapeniculus, andTrifur(Siphonostomatoida: Pennellidae), copepod parasites of marine fishes from Chile: species delimitation analyses using DNA barcoding and morphological evidence

Cited by 20 publications

References 64 publications

Black sea bass are a host in the developmental cycle of Lernaeenicus radiatus (Copepoda: Pennellidae): insights into parasite morphology, gill pathology and genetics

Black sea bass are a host in the developmental cycle of Lernaeenicus radiatus (Copepoda: Pennellidae): insights into parasite morphology, gill pathology and genetics

Quantification of complex modular architecture in plants

Non-destructive genome skimming for aquatic copepods

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