Integrin expression in malignant melanoma

Kramer, Randall H.; Vu, Mai; Cheng, Yao-Fen; Ramos, Daniel M.

doi:10.1007/bf00046843

Cited by 73 publications

(32 citation statements)

References 62 publications

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“…Integrin expression in tumours such as mnoma (Kramer et al, 1991), neuroblastoma (Favrot et al, 1991) and osteosarcoma (Lauri et al, 1991) has been extensively studied. Several studies have also focused on lung cancers (Damianovich et al, 1992Z Mette et al, 1993Suzuki et al, 1993), but have not clarly shown distinct patterns of integrin expresson among various histological types of lung cancer.…”

Section: Reagentsmentioning

confidence: 99%

Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines

Hirasawa

Shijubo²,

Uede³

et al. 1994

Br J Cancer

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Section: Reagentsmentioning

confidence: 99%

Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines

Hirasawa

Shijubo²,

Uede³

et al. 1994

Br J Cancer

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“…This complex, if present, should have been recovered in the EDTA-eluted fractions and visible in reducing SDS-gels as the 100-kDa a7 and 140-kDa f3I subunits. Instead, the only detectable integrin recovered in these fractions from the melanocyteswasal3l, (Figure9B).TheNaClwash fractions contained significant amounts of ,13 integrins ( Figure 9A); immunoprecipitation with anti-a chain-specific antibodies revealed that this mixture was composed of both a33,1 and a6#13 complexes ( Figure 9B (Kramer et al, 1991 (Kramer et al, 1991).…”

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confidence: 98%

Laminin-binding integrin alpha 7 beta 1: functional characterization and expression in normal and malignant melanocytes.

et al. 1991

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A novel integrin, a,#,, that specifically binds with high affinity to laminin has been identified on melanoma cells. This complex was purified from both human and murine melanoma cells by laminin-affinity chromatography, and the a7 subunit was recovered after gel electrophoresis. N-terminal amino acid sequence analysis of the a7 subunit from both human and mouse cells verifies that this integrin is distinct from other a chains in the #I family, although strikingly similar to the as subunit. By using specific proteolytically derived fragments of laminin, it was determined that the a7f1 complex binds selectively to the E8 region, which represents part of the long arm of laminin. In contrast, the receptor failed to bind to the P1 fragment, which contains the intersection of the short arms of laminin. Although the a7,,1 complex was commonly expressed in melanoma cells, this integrin was not detected in normal melanocytes, suggesting that a7 expression may be associated with malignant transformation. These results establish the existence of a novel integrin that binds to the E8 domain of laminin and appears to mediate cell adhesion to this ligand.

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“…Several studies have examined expression of the integrin family of adhesion molecules in cutaneous melanoma at various stages of tumour development. Levels of expression of different integrin subunits have been reported to increase during tumour progression, including a2,1 (Klein et al, 1991), a3 1 (Natali et al, 1993), a4pl (Schadendorf et al, 1993), a5,Bl (Danen et al, 1994), a6P1 (Natali et al, 1991) and a7 31 (Kramer et al, 1991 (Cheresh and Spiro, 1987). Thus, Albelda and colleagues (1990) noted that the ,3 subunit was only detected on VGP and metastases of cutaneous melanoma.…”

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confidence: 99%

Comparative analysis of integrins in vitro and in vivo in uveal and cutaneous melanomas

Marshall

Rutherford²,

Happerfield³

et al. 1998

Br J Cancer

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Summary Changes in integrin expression have been shown to be important for the growth and metastatic capacity of melanoma cells. In this study, we have examined the expression of av integrins by three uveal and four cutaneous malanoma lines. No lines expressed avP6 and only TXM1 3, a cutaneous line, expressed av,B8. All lines expressed avf5 and avP3 (four out of four cutaneous, two out of three uveal) or avfi1 (OM431, an uveal line). Thus, OM431 is the second uveal melanoma we have described that expresses avIl and this, we report again, functions as an alternative vitronectin/fibronectin receptor. Subcutaneous growth of cell lines in athymic mice correlated with an avp3-positive, avI1 -negative phenotype. Analysis of clinical material from cutaneous melanoma showed that although av expression was increased in 88% of metastases, this could not all be explained by up-regulation of av,B3, with only 2 out of eight skin metastases expressing this heterodimer.Using antibody SZ.21, which as we report here works in archival material, only 1 out of 15 uveal metastases expressed detectable P3. Thus, acquisition of avP3 expression, which has been implicated in cutaneous melanoma progression, may not be required for development of metastases from uveal melanoma or indeed for skin, as distinct from lymph node, metastases of cutaneous melanoma.Keywords: cell adhesion; ocular melanoma; skin melanoma Cutaneous melanoma is a tumour type whose incidence in Caucasian populations has increased dramatically over the past 80 years. The cancer generally is considered to evolve through a series of distinct pathological steps (Mastrangelo et al, 1985). Thus, melanocytic naevi progress to flat tumours that grow horizontally (radial growth phase, RGP) before they acquire the capacity to invade vertically (vertical growth phase, VGP) and then metastasize. Ocular melanomas, of which the most common are uveal melanomas, occur at about 10% the frequency of cutaneous tumours. These cancers do not seem to progress through the same stages of evolution, but the histological type of uveal melanoma determines the probable metastatic propensity. Thus, the 'spindle' forms rarely metastasize, whereas the 'epithelioid' types are highly metastatic and then usually spread preferentially to the liver (Shields and Shields, 1992).Disseminating cancer cells must interact with the extracellular matrix and this interaction is mediated principally by cell surface adhesion receptors, termed integrins (Hynes, 1992). Integrins are heterodimeric glycoproteins, consisting of a-chains non-covalently associated with ,B-chains, which are expressed at the cell surface. Several studies have examined expression of the integrin family of adhesion molecules in cutaneous melanoma at various stages of tumour development. Levels of expression of different integrin subunits have been reported to increase during tumour progression, including a2,1 (Klein et al, 1991), a3 1 (Natali et al, 1993), a4pl (Schadendorf et al, 1993), a5,Bl (Danen et al, 1994), a6P1 (Natali et al, 1991) ...

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Integrin expression in malignant melanoma

Cited by 73 publications

References 62 publications

Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines

Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines

Laminin-binding integrin alpha 7 beta 1: functional characterization and expression in normal and malignant melanocytes.

Comparative analysis of integrins in vitro and in vivo in uveal and cutaneous melanomas

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