Integrin-laminin interactions controlling neurite outgrowth from adult DRG neurons in vitro

Plantman, Stefan; Patarroyo, Manuel Elkín; Fried, Kaj; Domogatskaya, Anna; Tryggvason, Karl; Hammarberg, Henrik; Cullheim, Staffan

doi:10.1016/j.mcn.2008.05.015

Cited by 92 publications

(95 citation statements)

References 52 publications

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“…Contact chemoattraction or growth support is mediated by molecular families such as laminins, syndecans, and heparan sulfate proteoglycans (19,(90)(91)(92)(93). During development, axon growth on substrates is determined by combinatorial mixtures of attractive and repellent molecules, and gradients of such mixtures guide growth directionality (79,94).…”

Section: R E V I E W S E R I E S : G L I a A N D N E U R O D E G E N mentioning

confidence: 99%

Cell biology of spinal cord injury and repair

O’Shea¹,

Burda²,

Sofroniew³

2017

Journal of Clinical Investigation

452

349

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Section: R E V I E W S E R I E S : G L I a A N D N E U R O D E G E N mentioning

confidence: 99%

Cell biology of spinal cord injury and repair

O’Shea¹,

Burda²,

Sofroniew³

2017

Journal of Clinical Investigation

452

349

View full text Add to dashboard Cite

show abstract

“…They have functions in both the developing and mature nervous system, with roles in neurite outgrowth, synaptic plasticity, and peripheral nerve regeneration (Werner et al, 2000;Brakebusch and Fässler, 2005;Gardiner et al, 2005Gardiner et al, , 2007Webb et al, 2007;Lin et al, 2008;Plantman et al, 2008;McGeachie et al, 2011;Myers and Gomez, 2011). Integrins exist as heterodimers, composed of an ␣-and a ␤-subunit, with the specificity for their ligands being governed by the combined properties of both subunits.…”

Section: Introductionmentioning

confidence: 99%

ARF6 Directs Axon Transport and Traffic of Integrins and Regulates Axon Growth in Adult DRG Neurons

Eva¹,

Crisp²,

Marland³

et al. 2012

Journal of Neuroscience

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Integrins are involved in axon growth and regeneration. Manipulation of integrins is a route to promoting axon regeneration and understanding regeneration failure in the CNS. Expression of ␣9 integrin promotes axon regeneration, so we have investigated ␣9␤1 trafficking and transport in axons and at the growth cone. We have previously found that ␣9 and ␤1 integrins traffic via Rab11-positive recycling endosomes in peripheral axons and growth cones. However, transport via Rab11 is slow, while rapid transport occurs in vesicles lacking Rab11. We have further studied ␣9 and ␤1 integrin transport and traffic in adult rat dorsal root ganglion axons and PC12 cells. Integrins are in ARF6 vesicles during rapid axonal transport and during trafficking in the growth cone. We report that rapid axonal transport of these integrins and their trafficking at the cell surface is regulated by ARF6. ARF6 inactivation by expression of ACAP1 leads to increased recycling of ␤1 integrins to the neuronal surface and to increased anterograde axonal transport. ARF6 activation by expression of the neuronal guanine nucleotide exchange factors, ARNO or EFA6, increases retrograde integrin transport in axons and increases integrin internalization. ARF6 inactivation increases integrin-mediated outgrowth, while activation decreases it. The coordinated changes in integrin transport and recycling resulting from ARF6 activation or inactivation are the probable mechanism behind this regulation of axon growth. Our data suggest a novel mechanism of integrin traffic and transport in peripheral axons, regulated by the activation state of ARF6, and suggest that ARF6 might be targeted to enhance integrin-dependent axon regeneration after injury.

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“…They consist of an ␣ and a ␤ subunit that mediate numerous cellular functions including adhesion, proliferation, migration, synaptic plasticity, neurite outgrowth, and peripheral nerve regeneration (Pozzi et al, 1998;Werner et al, 2000;Brakebusch and Fässler, 2005;Gardiner et al, 2005Gardiner et al, , 2007Webb et al, 2007;Cingolani et al, 2008;Plantman et al, 2008;Moser et al, 2009). We recently expressed the tenascinbinding integrin ␣9 in sensory neurons with the intention of promoting regeneration of their axons in the damaged spinal cord (Andrews et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Rab11 and Its Effector Rab Coupling Protein Contribute to the Trafficking of β1 Integrins during Axon Growth in Adult Dorsal Root Ganglion Neurons and PC12 Cells

Eva¹,

Dassie²,

Caswell³

et al. 2010

J. Neurosci.

123

144

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Integrins play an important part in axon growth, but integrin traffic in neurons is poorly understood. Expression of the tenascin-Cbinding integrin ␣9 promotes axon regeneration. We have therefore studied the mechanism by which ␣9 integrin and its partner ␤1 are trafficked along axons and at the growth cone using adult DRG neurons and PC12 cells. We have focused on the small GTPase Rab11 and its effector Rab coupling protein (RCP), as they are involved in the long-range trafficking of ␤1 integrins in other cells. Rab11 colocalizes with ␣9 and other ␣ integrins and with ␤1 integrin in growth cones and axons, and immunopurified Rab11 vesicles contain ␣9 and ␤1. Endocytosed ␤1 integrins traffic via Rab11. However, Rab11 vesicles in axons are generally static, and ␣9 integrins undergo bouts of movement during which they leave the Rab11 compartment. In growth cones, ␣9 and ␤1 overlap with RCP, particularly at the growth cone periphery. We show that ␤1 integrin trafficking during neurite outgrowth involves Rab11 and RCP, and that manipulation of these molecules alters surface integrin levels and axon growth, and can be used to enhance ␣9 integrin-dependent neurite outgrowth. Our data suggest that manipulation of trafficking via Rab11 and RCP could be a useful strategy for promoting integrin-dependent axonal regeneration.

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Integrin-laminin interactions controlling neurite outgrowth from adult DRG neurons in vitro

Cited by 92 publications

References 52 publications

Cell biology of spinal cord injury and repair

Cell biology of spinal cord injury and repair

ARF6 Directs Axon Transport and Traffic of Integrins and Regulates Axon Growth in Adult DRG Neurons

Rab11 and Its Effector Rab Coupling Protein Contribute to the Trafficking of β1 Integrins during Axon Growth in Adult Dorsal Root Ganglion Neurons and PC12 Cells

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