Integrin-type fibronectin receptors of rat arterial smooth muscle cells: isolation, partial characterization and role in cytoskeletal organization and control of differentiated properties

Bottger, Bradford A.; Hedin, Ulf; Johansson, Staffan; Thyberg, Johan

doi:10.1111/j.1432-0436.1989.tb00743.x

Cited by 51 publications

(25 citation statements)

References 39 publications

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“…25,27 FN was found to exert its effect via the cell attachment sequence Arg-Gly-Asp and by interaction with a cell surface receptor belonging to the integrin family of proteins. 28 Hedin et al 26 demonstrated that a substrate composed of the cell attachment sequence of FN (Arg-Gly-Asp-Ser) is sufficient to promote the transition of VSMCs from the contractile to the synthetic phenotype. Therefore, we treated VSMCs with this fragment to promote the cells from the contractile to the synthetic phenotype.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Modulation by Fibronectin Enhances the Angiotensin II–Generating System in Cultured Vascular Smooth Muscle Cells

Fukuda

Satoh

et al. 2000

ATVB

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Abstract-We previously demonstrated that homogeneous cultures of vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats produce angiotensin II (Ang II) in response to increases in the levels of angiotensinogen, cathepsin D, and angiotensin-converting enzyme (ACE). The change of VSMCs from the contractile to the synthetic phenotype increased the amount of synthetic organelles, resulting in the production of proteases and growth factors. To evaluate the contribution of the synthetic phenotype to the generation of Ang II, we examined the effect of fibronectin (FN), which reportedly induces the synthetic phenotype, on the Ang II-generating system in VSMCs. Cultured VSMCs from Wistar-Kyoto rats were incubated with an active fragment of FN, Arg-Gly-Asp-Ser, for 24, 48, or 72 hours after synchronization of the cell cycle with 0.2% calf serum for 48 hours. Immunofluorescence and protein levels of ␣-smooth muscle (SM) actin and expression of SM22␣ mRNA, apparent in the contractile phenotype, were suppressed by FN, whereas expression of matrix Gla mRNA and osteopontin mRNA and protein, apparent in the synthetic phenotype, was increased. FN (1 to 1000 g/mL) dose-dependently increased DNA synthesis in the VSMCs, which was inhibited by the Ang II type 1 receptor antagonist CV-11974. Ang II-like immunoreactivity as determined by radioimmunoassay was significantly increased in conditioned medium from the VSMCs. In addition, mRNA for the Ang II-generating proteases cathepsin D and ACE was increased by FN. Expression of transforming growth factor-␤1, platelet-derived growth factor A-chain, and basic fibroblast growth factor mRNAs was also increased by FN. These results indicate that the changes accompanying the alteration to the synthetic phenotype in homogeneous cultures of VSMCs increase expression of proteases such as cathepsin D and ACE, which then produce Ang II, and that these changes increase expression of growth factors that then induce growth of VSMCs.

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Section: Discussionmentioning

confidence: 99%

Phenotypic Modulation by Fibronectin Enhances the Angiotensin II–Generating System in Cultured Vascular Smooth Muscle Cells

Fukuda

Satoh

et al. 2000

ATVB

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“…Mouse anti-human β1 integrin (clone 12G10) was obtained from Serotec (Oxford, UK). Rabbit-antiserum to β1 integrin was kindly provided by S. Johansson (Bottger et al, 1989) and mouse monoclonal antibody to vinculin was kindly provided by M. Glukhova. Mouse anti-human β1 integrin (clone 18) and phycoerythrin-conjugated CD29 (clone MAR4), anti-mouse β1 integrin (9EG7), anti-human fibronectin Ab (A245) and phycoerythrin-conjugated goat anti-mouse immunoglobulin G (IgG) were all obtained from BD Biosciences (Brøndby, Denmark).…”

Section: Antibodiesmentioning

confidence: 99%

ADAM12 induces actin cytoskeleton and extracellular matrix reorganization during early adipocyte differentiation by regulating β1 integrin function

Kawaguchi

Sundberg

Kveiborg

et al. 2003

Journal of Cell Science

126

112

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IntroductionAdult stem cells and progenitor cells can integrate and respond to appropriate extracellular stimuli in the form of hormones and growth factors, or contact with the extracellular matrix (ECM) and neighboring cells. The delicate balance between the dormancy of progenitor cells and their timely proliferation and differentiation is a crucial parameter in tissue homeostasis that is often perturbed in disease. In vitro studies and animal implant experiments have revealed the multipotential nature of mesenchymal stem cells, which contribute to the regeneration of mesenchymal tissues such as bone, cartilage, muscle, tendon, stroma and adipose tissues (Pittenger et al., 1999;Prockop, 1997).Adipogenesis is a complex process characterized by the strict temporal regulation of multiple and interacting signaling events that ultimately lead to the expression of adipocytespecific genes (Gregoire et al., 1998;Rosen and Spiegelman, 2000). A cascade of transcription factors is induced that involves the sequential activation of the CCAAT/enhancerbinding proteins (C/EBPs) and the peroxisome proliferatoractivated receptor γ (PPARγ), which leads to the activation of several genes, such as those responsible for lipid transport and metabolism. Initially, fibroblastic preadipocytes stop dividing and acquire a rounded morphology. The change of shape from fibroblastic preadipocytes to rounded, mature adipocytes is accompanied by changes in cytoskeletal organization and contacts with the ECM. The expression of fibronectin, integrins, actin and several cytoskeletal proteins is downregulated during adipogenesis (Rodriguez Fernandez and Ben-Ze'ev, 1989;Spiegelman and Farmer, 1982). In fact, the disruption of contacts with the ECM is a requirement for adipocyte differentiation (Spiegelman and Ginty, 1983).Our understanding of the molecular events involved in adipogenesis is based mainly on in vitro, cell culture models, 3893Changes in cell shape are a morphological hallmark of differentiation. In this study we report that the expression of ADAM12, a disintegrin and metalloprotease, dramatically affects cell morphology in preadipocytes, changing them from a flattened, fibroblastic appearance to a more rounded shape. We showed that the highest levels of ADAM12 mRNA were detected in preadipocytes at the critical stage when preadipocytes become permissive for adipogenic differentiation. Furthermore, as assessed by immunostaining, ADAM12 was transiently expressed at the cell surface concomitant with the reduced activity of β1 integrin. Co-immunoprecipitation studies indicated the formation of ADAM12/β1 integrin complexes in these preadipocytes. Overexpression of ADAM12 at the cell surface of 3T3-L1 preadipocytes achieved by transient transfection or retroviral transduction led to the disappearance of the extensive network of actin stress fibers that are characteristic of these cells, and its reorganization into a cortical network located beneath the cell membrane. The cells became more rounded, exhibited fewer vinculin-positive focal ad...

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“…Adhesion to and migration on ECM proteins are mediated by different integrin heterodimers. To date, several β 1 (27)(28)(29)(30)(31)54) and β 3 (55,56,60,61) integrins have been identified on smooth muscle cells. Although integrins of the β 1 family appear to be the dominant integrins responsible for VSMC adhesion to the ECM (27,56), and also appear to be involved in VSMC migration (20,31,55), migration of VSMCs has been noted to depend on β 3 integrins (55,56).…”

Section: Figure 11mentioning

confidence: 99%

“…The chemotactic growth factors PDGF (18,19) and basic fibroblast growth factor (bFGF) (20,21) are likely to direct VSMC migration, and the local extracellular matrix (ECM) environment may provide an additional level of motility regulation (22)(23)(24)(25). Integrin cell-surface receptors serve as a link between ECM and the cytoskeleton (26), and both adhesion and migration can be regulated by altering integrin expression patterns (27)(28)(29)(30)(31). Whereas these exogenous regulators of cell migration are relatively well described, little is known about the nuclear proteins that function to coordinate cell growth and motility.…”

mentioning

confidence: 99%

Regulation of smooth muscle cell migration and integrin expression by the Gax transcription factor

Witzenbichler

Kureishi²,

Luo³

et al. 1999

J. Clin. Invest.

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Integrin-type fibronectin receptors of rat arterial smooth muscle cells: isolation, partial characterization and role in cytoskeletal organization and control of differentiated properties

Cited by 51 publications

References 39 publications

Phenotypic Modulation by Fibronectin Enhances the Angiotensin II–Generating System in Cultured Vascular Smooth Muscle Cells

Phenotypic Modulation by Fibronectin Enhances the Angiotensin II–Generating System in Cultured Vascular Smooth Muscle Cells

ADAM12 induces actin cytoskeleton and extracellular matrix reorganization during early adipocyte differentiation by regulating β1 integrin function

Regulation of smooth muscle cell migration and integrin expression by the Gax transcription factor

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