Integrin α8 Is Abundant in Human, Rat, and Mouse Trophoblasts

Herdl, Sebastian; Huebner, Hanna; Volkert, Gudrun; Marek, Ines; Menendez-Castro, Carlos; Noegel, Stephanie C.; Ruebner, Matthias; Rascher, Wolfgang; Hartner, Andrea; Fahlbusch, Fabian

doi:10.1177/1933719116689597

Cited by 5 publications

(2 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It may serve as a marker for placental bed remodeling [24]. Decreased expression of OPN has been reported to be involved in the pathogenesis of preeclampsia [23][24][25]. CCR2 are involved in Th1 and Th2 immunity activation which might contribute to the development of preeclampsia [26].…”

Section: Discussionmentioning

confidence: 99%

Identification of Key Genes and Long Noncoding RNA‐Associated Competing Endogenous RNA (ceRNA) Networks in Early‐Onset Preeclampsia

Zhang

Wang

Zhang

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Background. Preeclampsia (PE) is a pregnancy-specific hypertension syndrome and is one of the leading causes of maternal and perinatal morbidity and mortality. Long noncoding RNAs (lncRNAs) have been reported to be abnormally expressed in many diseases, including preeclampsia. The present study is aimed at identifying the key genes and lncRNA-associated competing endogenous RNA (ceRNA) networks in early-onset preeclampsia (EOPE). Methods. We investigated expression profiles of differentially expressed lncRNAs (DElncRNAs) and genes (DEGs) in placental tissues of EOPE and healthy controls with Human LncRNA Array v4. The potential functions of DEGs and DElncRNAs were predicted using the clusterProfiler package. The lncRNA-mRNA coexpression network was constructed via Pearson’s correlation coefficient. The protein-protein interaction (PPI) network of DEGs was constructed, and the hub genes were obtained using the STRING database and Cytoscape. The ceRNA networks were constructed based on miRWalk and LncBase v2. qRT-PCR was performed to confirm the expression of lncRNA MIR193BHG, PROX1-AS1, and GATA3-AS1. ROC curves were performed to assess the clinical value of lncRNA MIR193BHG, PROX1-AS1, and GATA3-AS1 in the diagnosis of EOPE. Results. We found 6 hub genes (SPP1, CCR2, KIT, ENG, ACKR1, and FLT1) altered in placental tissues of EOPE and constructed a ceRNA network, including 21 lncRNAs, 3 mRNAs, and 69 miRNAs. The expression of lncRNA MIR193BHG and GATA3-AS1 were elevated and showed good clinical values for diagnosing EOPE. Conclusion. This study provides novel insights into the lncRNA-related ceRNA network in EOPE and identified two lncRNAs as potential prognostic biomarkers in EOPE.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Key Genes and Long Noncoding RNA‐Associated Competing Endogenous RNA (ceRNA) Networks in Early‐Onset Preeclampsia

Zhang

Wang

Zhang

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Moreover, eNOS deficiency seems to be associated with an increased inflammatory state [31][32][33][34]. We chose eNOS −/− mice over various other rodent models of PE (reviewed by [18]) as placentas of this model lack gross anatomic alterations [28] similar to our low protein rat model [66]. Each group of mice was mated, and the presence of a copulation plug was denoted as day 0.5 of pregnancy.…”

Section: Mouse Model With Pe/iugr-like Featuresmentioning

confidence: 99%

Expression of Retinoid Acid Receptor-Responsive Genes in Rodent Models of Placental Pathology

Mocker

Schmidt

Huebner

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

In humans, retinoic acid receptor responders (RARRES) have been shown to be altered in third trimester placentas complicated by the pathologies preeclampsia (PE) and PE with intrauterine growth restriction (IUGR). Currently, little is known about the role of placental Rarres in rodents. Therefore, we examined the localization and expression of Rarres1 and 2 in placentas obtained from a Wistar rat model of isocaloric maternal protein restriction (E18.5, IUGR-like features) and from an eNOS-knockout mouse model (E15 and E18.5, PE-like features). In both rodent models, Rarres1 and 2 were mainly localized in the placental spongiotrophoblast and giant cells. Their placental expression, as well as the expression of the Rarres2 receptor chemokine-like receptor 1 (CmklR1), was largely unaltered at the examined gestational ages in both animal models. Our results have shown that RARRES1 and 2 may have different expression and roles in human and rodent placentas, thereby underlining immanent limitations of comparative interspecies placentology. Further functional studies are required to elucidate the potential involvement of these proteins in early placentogenesis.

show abstract