Integrin β3, a RACK1 interacting protein, is critical for porcine reproductive and respiratory syndrome virus infection and NF-κB activation in Marc-145 cells

Yang, Chao; Lan, Rui; Wang, Xiaochun; Zhao, Qian; Li, Xidan; Bi, Jianzhong; Wang, Jing; Yang, Guliang; Lin, Yingbo; Liu, Jianping; Yin, Gefen

doi:10.1016/j.virusres.2020.197956

Cited by 11 publications

(4 citation statements)

References 59 publications

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“…ITGB3 (integrin subunit β3) is enriched in this pathway and plays a crucial role in coordinating immune responses, which is widely distributed in the spleen. Previous research has emphasized the positive correlation between ITGB3 and porcine PRRSV infection and transmission [60][61][62]. Among the three pig breeds, the expression level of the ITGB3 gene is highest in Ningxiang pigs and lowest in F 1 offspring.…”

Section: Discussionmentioning

confidence: 98%

Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs

Song,

Zhang,

Gao

et al. 2024

Genes

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To investigate the differential immunology in Ningxiang and Berkshire pigs and their F1 offspring (F1 offspring), physiological and biochemical indicators in the plasma and spleen were analyzed. Then, transcriptomic analysis of the spleen identified 1348, 408, and 207 differentially expressed genes (DEGs) in comparisons of Ningxiang vs. Berkshire, Berkshire vs. F1 offspring, and Ningxiang vs. F1 offspring, respectively. In Ningxiang vs. Berkshire pigs, the gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEGs included CD163, MARCO, CXCL14, CCL19, and PPBP, which are associated with immunity. GO and KEGG analyses were also conducted comparing F1 offspring and their parents. The DEGs, including BPIFB1, HAVCR2, CD163, DDX3X, CCR5, and ITGB3, were enriched in immune-related pathways. Protein–protein interaction (PPI) analysis indicated that the EGFR and ITGA2 genes were key hub genes. In conclusion, this study identifies significant immune DEGs in different pig breeds, providing data to support the exploration of breeding strategies for disease resistance in local and crossbred pig populations.

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Section: Discussionmentioning

confidence: 98%

Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs

Song,

Zhang,

Gao

et al. 2024

Genes

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“…Therefore, we used miRNA sequences of chicken and zebra finch, which are available in miRbase, to search for conserved miRNAs and detected 437 known miRNAs. Similarly, several studies were also mapped to precursor/mature miRNAs of chicken or other animals in miRbase to annotate conserved miRNAs ( Cui et al., 2018 ; Wu et al., 2018 ; Wu et al., 2019a ; Yang et al., 2020 ). In addition, miRNA length showed that the majority had a length of 23 nt and the first nucleotide base revealed U predominance in the identified miRNAs ( Figure 2 ), which is consistent with the findings of other studies ( Chen et al., 2021 ; Zhang et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…Integrins act as entry receptors for multiple viruses, including ZIKV ( Seong et al., 2020 ), HIV ( Dalvi et al., 2016 ), and West Nile virus (WNV) ( Schmidt et al., 2013 ), and are also involved in immunity response ( Trentelman et al., 2020 ). Moreover, a recent study reported that ITGB3 plays an essential role in the activation of NF-κB and Porcine reproductive and respiratory syndrome virus (PRRSV) infection ( Yang et al., 2020a ). Accordingly, our data demonstrated that overexpression of ITGB3 promoted DHAV-1 replication.…”

Section: Discussionmentioning

confidence: 99%

Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection

Sui

Zhang

Jiang

et al. 2022

Front. Cell. Infect. Microbiol.

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Duck hepatitis A virus 1 (DHAV-1) is a highly contagious etiological agent that causes acute hepatitis in young ducklings. MicroRNAs (miRNAs) play important regulatory roles in response to pathogens. However, the interplay between DHAV-1 infection and miRNAs remains ambiguous. We characterized and compared miRNA and mRNA expression profiles in duck embryo fibroblasts cells (DEFs) infected with DHAV-1. In total, 36 and 96 differentially expressed (DE) miRNAs, and 4110 and 2595 DE mRNAs, were identified at 12 and 24 h after infection. In particular, 126 and 275 miRNA–mRNA pairs with a negative correlation were chosen to construct an interaction network. Subsequently, we identified the functional annotation of DE mRNAs and target genes of DE miRNAs enriched in diverse Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, which may be important for virus resistance, cell proliferation, and metabolism. Moreover, upregulated miR-222a could negatively regulate DHAV-1 replication in DEFs and downregulate integrin subunit beta 3 (ITGB3) expression by targeting the 3′ untranslated region (3′UTR), indicating that miR-222a may modulate DHAV-1 replication via interaction with ITGB3. In conclusion, the results reveal changes of mRNAs and miRNAs during DHAV-1 infection and suggest miR-222a as an antiviral factor against DHAV-1.

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“…The widespread presence of a scaffolding protein receptor for activated protein kinase C1 (RACK1), which exhibits regulatory activity with protein kinase C (PKC) in both prokaryotes and eukaryotes, has garnered increasing attention and scrutiny [ 1 , 2 ]. As a highly conserved member of the tryptophan-aspartic acid domain (WD) repeat protein family, RACK1 participates in many biological processes, such as proliferation, cell migration, apoptosis, development, cellular adaptation processes, and immune responsiveness [ 1 , 3 , 4 , 5 , 6 , 7 , 8 ]. In addition, RACK1 is known as an adaptor protein in multiple intracellular signal transduction pathways, such as the PKC/RACK pathway [ 9 ], the cyclic AMP-specific phosphodiesterase pathway [ 10 , 11 ], the tyrosine kinase-phosphatase pathway [ 12 ], and the Aryl hydrocarbon receptor (AhR) signaling pathway [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Characterization, and Expression of a Receptor for Activated Protein Kinase C1 (RACK1) Gene in Exopalaemon carinicauda Zoea Larvae under Aroclor 1254 Stress

Cai,

Hu,

Guo

et al. 2024

Biology

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The receptor for activated protein kinase C1 (RACK1) belongs to the typical WD repeat family, which is extremely conservative and important in multiple signal transduction pathways related to growth and development that coordinate the intracellular role of various life activities. As a novel protein with versatile functions, it was found in a variety of organisms. In a previous study, we identified the RACK1 sequence of white shrimp from transcriptome data. In this study, we employed specialized bioinformatics software to conduct an in-depth analysis of EcRACK1 and compare its amino acid sequence homology with other crustaceans. Furthermore, we investigated the expression patterns of RACK1 at different developmental stages and tissues, as well as at various time points after exposure to Aroclor 1245, aiming to elucidate its function and potential response towards Aroclor 1245 exposure. The length of EcRACK1 is 957 nucleotides, which encodes 318 amino acids. Moreover, there were seven typical WD repeats in EcRACK1, which have more than a 96% sequence identity with the RACK1 proteins of Penaeus. The results of tissue expression and spatiotemporal expression showed that it was significantly increased in the II and IV stages, but had a significant tissue specificity in the hepatopancreas, spermary, and muscle tissues of E. carinicauda, adult stage. Compared to the control, EcRACK1 was significantly induced in E. carinicauda zoea larvae exposed to Aroclor 1254 for 6, 10, 20, and 30 d (p < 0.05). These results suggested that EcRACK1 may play an important role in the larval development and environmental defense of E. carinicauda.

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Integrin β3, a RACK1 interacting protein, is critical for porcine reproductive and respiratory syndrome virus infection and NF-κB activation in Marc-145 cells

Abstract: Although shared co-first author. Chao Yang performed more experiments than Rui Lan.

Cited by 11 publications

References 59 publications

Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs

Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs

Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection

Molecular Cloning, Characterization, and Expression of a Receptor for Activated Protein Kinase C1 (RACK1) Gene in Exopalaemon carinicauda Zoea Larvae under Aroclor 1254 Stress

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