Integrity and redox properties of homogeneous and heterogeneous DNA films on gold surface probed by cyclic voltammetry

Yau, Hellas C.M.; Chan, Hing Leung; Sui, Sen‐Fang; Yang, Mengsu

doi:10.1016/s0040-6090(02)00448-0

Cited by 18 publications

(8 citation statements)

References 17 publications

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“…It is well-known that many metal surfaces can interact with RNA, sometimes irreversibly, and this would result in denaturing the active structure. For example, previous reports of DNA bound to Au substrates suggest that binding to the surface by the DNA nucleobases − can be significant making it uncertain if in fact active folded RNA sequences found from solution in vitro selection experiments would perform when attached to an Au surface. Herein we describe using Pdases as a prototype RNA catalyst bound to an Au surface.…”

Section: Introductionmentioning

confidence: 99%

RNA-Mediated Synthesis of Palladium Nanoparticles on Au Surfaces

Liu

Gugliotti

et al. 2006

Langmuir

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RNA catalysts for the shape-controlled synthesis of Pd particles from the precursor complex trisdibenzylideneacetone dipalladium ([Pd2(DBA)3] were recently discovered in our laboratory (J. Am. Chem. Soc. 2005, 127, 17814-17818). In the work described here, RNA codes for hexagonal Pd platelets and Pd cubes were covalently immobilized on gold surfaces and evaluated for their activity toward particle synthesis. When coupled to gold via oligoethylene glycol linkers, both RNA sequences were able to catalyze the formation of Pd particles with the same shape control previously observed in solution. For low surface coverages, the average distance between RNA molecules on the surface was estimated at ca. 300 nm, yet large (e.g., dimensions of hundreds of nanometers) Pd hexagons and cubes still formed. This surprising result suggests that a single RNA molecule may be sufficient for nucleating and controlling the shapes of these particles. Finally, the use of surface-bound RNA as a tool for directing the orthogonal synthesis of materials on surfaces was demonstrated. Patterning the RNA code for Pd hexagons next to the code for Pd cubes, followed by incubation in a solution containing [Pd2(DBA)3], resulted in the spontaneous formation of spatially distinct spots of hexagonal and cubic particles.

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Section: Introductionmentioning

confidence: 99%

RNA-Mediated Synthesis of Palladium Nanoparticles on Au Surfaces

Liu

Gugliotti

et al. 2006

Langmuir

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“…Longer chain alkanethiols are expected to form better-packed monolayers [29] and the advantages of their use as backfilling molecules have been reported [30,31]. The efficacy of the blocking layers correlates well with indicators of the SAM quality, such as the water contact angle and electrochemical blocking of redox reactions at the surface (data not shown), but the characteristics of the exposed head groups were also found to be of importance.…”

Section: Resultsmentioning

confidence: 92%

“…Short DNA fragments are attached to the surface first, which then act as anchor sites for the larger structure. The short DNA strands can be bound to the surface in a controlled fashion [30], while providing a stable and highly specific anchor point for subsequent attachment of the larger DNA molecule by self-assembly. The approach allows the multi-point attachment of single biomolecules.…”

Section: Discussionmentioning

confidence: 99%

DNA self-assembly-driven positioning of molecular components on nanopatterned surfaces

2016

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We present a method for the specific, spatially targeted attachment of DNA molecules to lithographically patterned gold surfaces—demonstrated by bridging DNA strands across nanogap electrode structures. An alkanethiol self-assembled monolayer was employed as a molecular resist, which could be selectively removed via electrochemical desorption, allowing the binding of thiolated DNA anchoring oligonucleotides to each electrode. After introducing a bridging DNA molecule with single-stranded ends complementary to the electrode-tethered anchoring oligonucleotides, the positioning of the DNA molecule across the electrode gap, driven by self-assembly, occurred autonomously. This demonstrates control of molecule positioning with resolution limited only by the underlying patterned structure, does not require any alignment, is carried out entirely under biologically compatible conditions, and is scalable.

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“…The decrease of the intensity of the oxidation current of a single strand (ss, without target ODN) and a double strand (ds, with complementary target ODN) can be explained by the polar hydrogen bonds at the polymer's surface, which form a potential barrier to the rotation of the polymer backbone and can be attributed to the bulky conformational modifications made along the conjugated polymer backbone ( Figure 6A) [23][24][25].…”

Section: Odn Immobilizationmentioning

confidence: 99%

Electrochemical Polymerization and Investigation of Properties of Poly(5‐(4′‐aminophenylamino)‐10,20‐diphenylporphyrin)

et al. 2011

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The preparation and electropolymerization of a new porphyrin monomer with an amino group connecting the porphyrin core and aniline unit were studied. Cyclic voltammetry, FTIR spectroscopy, and a tandem quadrupole/time of flight mass spectrometry with matrix-assisted laser desorption/ionization source (MALDI-QqTOF) were used for the characterization of poly(5-(4'-aminophenylamino)-10,20-diphenylporphyrin) (PAPADPP). The PAPADPP formed a smooth layer on the Indium tin oxide (ITO) electrode and grew perpendicular to the electrode surface. An oligonucleotide (ODN)-probe was physisorbed onto the PAPADPP film and tested for hybridization with complementary and noncomplementary ODN segments. Biological recognition was monitored by comparison of the electrochemical signals (cyclic voltammogram) from single and double strand state oligonucleotides. The morphology of the physisorbed ODN-probe and its hybridization were observed on ITO electrodes using AFM and SEM.

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Integrity and redox properties of homogeneous and heterogeneous DNA films on gold surface probed by cyclic voltammetry

Cited by 18 publications

References 17 publications

RNA-Mediated Synthesis of Palladium Nanoparticles on Au Surfaces

RNA-Mediated Synthesis of Palladium Nanoparticles on Au Surfaces

DNA self-assembly-driven positioning of molecular components on nanopatterned surfaces

Electrochemical Polymerization and Investigation of Properties of Poly(5‐(4′‐aminophenylamino)‐10,20‐diphenylporphyrin)

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