Integrity and wound healing of rainbow trout intestinal epithelial cell sheets at hypo-, normo-, and hyper-thermic temperatures

Pumputis, Patrick G.; Braley, Eryn; Hamilton, Mark E.; Dayeh, Vivian R.; Lee, Lucy E. J.; Bols, Niels C.

doi:10.1016/j.jtherbio.2021.103147

Cited by 4 publications

(4 citation statements)

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“…Fishes, being the most diverse group of animals comprising over half of the vertebrate species, are used as a recommended scientific model to study different biological processes (Luer & Walsh, 2018; Pumputis et al, 2022; Schartl, 2014). They may adapt to diverse environmental conditions, which make them suitable for various experimentations, such as medaka and zebrafish have successfully been employed for elucidating gene function and disease mechanisms in humans (Harris et al, 2014; Lin et al, 2016; Lleras‐Forero et al, 2020; Metz et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus

Pande,

Thakuria,

Kushwaha

et al. 2024

Cell Biology International

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Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named “RBT‐H.” The cell line was authenticated using karyotyping and cytochrome c oxidase subunit I (COI) gene sequencing. The karyotype demonstrated diploid chromosome number (2n) as 62 and the sequence of partial COI gene was 99.84% similar to rainbow trout COI data set, both suggesting the origin of RBT‐H from the rainbow trout. The heart cell line was mycoplasma‐free and found to be refractory to infection with the Tilapia lake virus. The RBT‐H cell line is deposited in the National Repository of Fish Cell Line (NRFC) at ICAR‐NBFGR, Lucknow, India, with Accession no. NRFC0075 for maintenance and distribution to researchers on request for R&D.

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Section: Introductionmentioning

confidence: 99%

A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus

Pande,

Thakuria,

Kushwaha

et al. 2024

Cell Biology International

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“…This is a general ecological concern, and a particular problem for aquaculture because fish such as salmon and rainbow trout in marine and freshwater net cages cannot avoid extreme temperatures behaviorally. 4,[6][7][8][9][10] The rainbow trout (Oncorhynchus mykiss) is an economically important cold-water fish worldwide and is highly sensitive to temperature changes. 11,12 High temperatures in summer months have become the biggest threat to rainbow trout farming.…”

Section: Introductionmentioning

confidence: 99%

“…Temperature stress is more complex for ectotherms because temperature ranges are wider, and with climate change and an increase in temperature variability, more frequent violations of temperature limits can be expected to lead to both cold and heat shock. This is a general ecological concern, and a particular problem for aquaculture because fish such as salmon and rainbow trout in marine and freshwater net cages cannot avoid extreme temperatures behaviorally 4,6‐10 …”

Section: Introductionmentioning

confidence: 99%

Effect of climate change on fillet quality and shelf‐life of Oncorhynchus mykiss under controlled conditions

Alak,

Kara,

Akköse

et al. 2023

J Sci Food Agric

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BackgroundTemperature, which affects numerous physiological processes, has been described as the 'main ecological factor' for fish. The aim of this modelling study is to explore the impact of climate‐induced temperature changes on fish fillet quality and shelf life.ResultsThe obtained results showed that temperature stress in rainbow trout effects ash and moisture and inhibits myofibril fragmentation in fillet. However, with the increase in temperature, there was a decrease in the total amount of saturated fatty acids (∑SFA) and significant increases in the total amount of omega 3 (∑n3) and 22:6n‐3 (DHA). It was determined that temperature increase had a negative effect on color, texture, water holding capacity, water activity, pH, lactic acid and glycogen levels in fillets, while it had a positive effect on the delay of microbial spoilage especially in cold storage.ConclusionThis study suggest that the effects of climate change on products quality and shelf life infish take specific research community attention. However, it also highlights knowledge gaps to guide future research in this emerging field.This article is protected by copyright. All rights reserved.

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“…This is only possible by monitoring individual responses and physiological functions. Therefore, experimental biology is particularly important in understanding the effects of global warming on fish [ 19 , 20 ]. As a result of the sensitivity of physiological parameters and to adapt to temperature changes, fish alter their endocrine processes, metabolic rates, immune and antioxidant systems [ 7 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of climate change on hematotoxicity/hepatoxicity oxidative stress, Oncorhynchus mykiss, under controlled conditions

Alak,

Özgeriş,

Uçar

et al. 2023

PLoS ONE

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Described as the ’main ecological factor’, temperature, strongly affects the physiological stress responses of fish. In order to evaluate the effects of temperature variations on fish culture and food value chain, the present study was designed as a climate change model. Furthermore, the present study provides a theoretical basis for a better understanding of the mechanisms of the environmentally induced changes. In this direction, we examined the blood physiology and oxidative stress responses induced by temperature variation in the rainbow trout, a temperature-sensitive cold-water fish. The obtained results showed that climate changes promoted the inhibited activities’ expressions and the development of potential tissue and hematological defense mechanisms against temperature-induced toxic damage. This study showed that climate change could be a subset of the studies on the stress physiology in aquaculture, which can be developed for new experimental designs and research collaborations. Furthermore, it highlights knowledge gaps to guide future research in this emerging field.

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Integrity and wound healing of rainbow trout intestinal epithelial cell sheets at hypo-, normo-, and hyper-thermic temperatures

Cited by 4 publications

References 50 publications

A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus

A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus

Effect of climate change on fillet quality and shelf‐life of Oncorhynchus mykiss under controlled conditions

Effect of climate change on hematotoxicity/hepatoxicity oxidative stress, Oncorhynchus mykiss, under controlled conditions

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