Integrity of the plasma membrane, the acrosomal membrane, and the mitochondrial membrane potential of sperm in Nelore bulls from puberty to sexual maturity

Reis, L. S. L. S.; Ramos, A. A.; Camargos, A. S.; Oba, Eunice

doi:10.1590/1678-4162-8748

Cited by 12 publications

(8 citation statements)

References 22 publications

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“…This trend was also observed for frozen-thawed samples, although the changes were not significant. These results are in agreement with observations from previous research where sperm cell motility in fresh samples were positively correlated with increasing age in Holstein bulls (Devkota et al, 2008;Murphy et al, 2018) and Nelore bulls (Reis et al, 2016). Overall, the results from sperm quality analyses show that sperm cells from NR bulls at 17 months of age displayed higher sperm quality compared to 14 months of age.…”

Section: Discussionsupporting

confidence: 92%

Sperm DNA Hypomethylation Proximal to Reproduction Pathway Genes in Maturing Elite Norwegian Red Bulls

et al. 2020

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Genomic selection in modern farming demands sufficient semen production in young bulls. Factors affecting semen quality and production capacity in young bulls are not well understood; DNA methylation, a complicated phenomenon in sperm cells, is one such factors. In this study, fresh and frozen-thawed semen samples from the same Norwegian Red (NR) bulls at both 14 and 17 months of age were examined for sperm chromatin integrity parameters, ATP content, viability, and motility. Furthermore, reduced representation bisulfite libraries constructed according to two protocols, the Ovation R RRBS Methyl-Seq System (Ovation method) and a previously optimized gel-free method and were sequenced to study the sperm DNA methylome in frozen-thawed semen samples. Sperm quality analyses indicated that sperm concentration, total motility and progressivity in fresh semen from 17 months old NR bulls were significantly higher compared to individuals at 14 months of age. The percentage of DNA fragmented sperm cells significantly decreased in both fresh and frozen-thawed semen samples in bulls with increasing age. Libraries from the Ovation method exhibited a greater percentage of read loss and shorter read size following trimming. Downstream analyses for reads obtained from the gel-free method revealed similar global sperm DNA methylation but differentially methylated regions (DMRs) between 14-and 17 months old NR bulls. The majority of identified DMRs were hypomethylated in 14 months old bulls. Most of the identified DMRs (69%) exhibited a less than 10% methylation difference while only 1.5% of DMRs exceeded a 25% methylation difference. Pathway analysis showed that genes annotated with DMRs having low methylation differences (less than 10%) and DMRs having between 10 and 25% methylation differences, could be associated with important hormonal signaling and sperm function relevant pathways, respectively. The current research shows that RRBS in parallel with routine sperm quality analyses could be informative in reproductive capacity of young NR bulls. Although global sperm DNA methylation levels in 14 and 17 months old NR bulls were similar, regions with low and varying levels of DNA methylation differences can be identified and linked with important sperm function and hormonal pathways.

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Section: Discussionsupporting

confidence: 92%

Sperm DNA Hypomethylation Proximal to Reproduction Pathway Genes in Maturing Elite Norwegian Red Bulls

et al. 2020

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“…On the other hand, it is conceivable that the higher sperm membrane integrity observed in this work after supplementation of the vegetal extract in turn improved both viability and progressive motility of sperm. Accordingly, it is widely known that maintenance of spermatic plasma membrane is required for both vitality and motility, which are landmarks for male fertility throughout species, as indicated by Fraser et al (2001), Ahmad et al (2015), Reis et al (2016). Thus, conservation of plasma membrane structure, as a consequence of increased antioxidant capacity upon M. oleifera extract addition here, also appears as a requirement for sperm viability and progressive motility in ram semen.…”

Section: Discussionmentioning

confidence: 86%

Effect ofMoringa oleiferaseed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen

Carrera‐Chávez

Jiménez-Aguilar

Acosta-Pérez

et al. 2020

Journal of Applied Animal Research

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2020) Effect of Moringa oleifera seed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen, ABSTRACT Semen cryopreservation damages sperm due to oxidative stress. This study evaluated the antioxidant capacity of Moringa oleifera seed extract in cryopreserved ram semen and the impact of the extract on sperm characteristics. Semen from eight hair rams (four rams per sampling) was allocated into four groups, according to their treatment prior to cryopreservation: Control (no extract), 0.5 (M0.5), 5.0 (M5.0), and 10.0 (M10) mg/mL of M. oleifera extract. The antioxidant activity (ferric reducing antioxidant power, FRAP) and the spermatic characteristics (sperm viability; progressive motility; fast motility; slow motility; acrosome damage; membrane damage; and mitochondrial activity) were assessed post-thawing. Variables were evaluated with analysis of variance followed by Tukey test. While no significant differences were detected in acrosomal damage, mitochondrial activity, fast-, or slow motility, the antioxidant activity was higher (P < 0.05) in M0.5 and M5 treatments. Viability and progressive motility increased in the M0.5 group (P < 0.05), whereas sperm membrane damage was lower (P < 0.05) in the same treatment. In conclusion, supplementation of ram semen with M. oleifera seed extract enhances antioxidant activity, sperm membrane integrity, viability, and progressive motility after thawing. This suggests that M. oleifera extract could be used as an antioxidant to improve the outcome of semen cryopreservation. Highlights. It is widely known that semen cryopreservation induces sublethal damage to sperm, deteriorating spermatic characteristics, which is largely attributed to oxidative stress. . There are scarce studies regarding the use of plant extracts as a replacement for conventional antioxidants to conserve sperm viability in cryopreserved ram semen. . Addition of Moringa oleifera seed extract to a concentration of 0.5 and 5.0 mg/mL prior to ram semen freezing increased antioxidant activity after cryopreservation. . M. oleifera seed extract at 0.5 mg/mL decreased post-thawing damage to the sperm membrane, increasing both viability and progressive motility. . M. oleifera seed extract could be potentially used as a replacement for conventional antioxidants added to maintain sperm viability in cryopreserved ram semen. ARTICLE HISTORY

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“…Both low fresh semen (sperm's cell individual motility less than 40%) from nine bulls and good fresh semen quality from three bulls were collected via artificial vagina at two ejaculates/bull/week. Measuring of ejaculate volume, next concentration of sperm was calculated by a Neubauer Haemocytometer counting chamber was used for sperm concentration (28). Low and good semen quality was weekly collected from 12 Holstein bulls (9 for low and 3 for good semen quality) for 16 weeks experimental periods.…”

Section: Methodsmentioning

confidence: 99%

Effect of Swim-Up and Glass Wool Techniques, With Adding Antioxidants to Tris Extender on Improving Post-Cryopreserved Total Sperm Characteristics in Straw and Freezability Percentage for Low Semen Quality of Holstein Bulls

Hassan¹

2021

Iraqi J. Agric. Sci.

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This study was conducted to investigate the effect of swim-up and glass wool as sperm separation techniques with adding vitamin E and superoxide dismutase (SOD) to Tris extender on improving post-cryopreserved total sperm characteristics in straw and freezability percentage for low semen quality of Holstein bulls. Low and good of semen were extended using Tris extender. Good semen quality (GSQ) was divided into 3 groups (L1; Tris extender, L2; 2 mM vitamin E, L3; 200 IU SOD) Low semen quality (LSQ) was divided into two main groups, and subdivided into 3 sub-groups (L4; Tris extender, L5; 2 mM vitamin E, L6; 200 IU SOD). In the second main group, swim-up and glass wool techniques were used with adding vitamin E and SOD and subdivided into 3 sub-groups with each technique, and referred to L7, L8 and L9 for swim-up technique and L10, L11 and L12 for glass wool technique. Sperms with good motile intact acrosome and plasma membrane normal morphology of sperm were obtained using the glass wool separation technique with adding vitamin E.

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Integrity of the plasma membrane, the acrosomal membrane, and the mitochondrial membrane potential of sperm in Nelore bulls from puberty to sexual maturity

Cited by 12 publications

References 22 publications

Sperm DNA Hypomethylation Proximal to Reproduction Pathway Genes in Maturing Elite Norwegian Red Bulls

Sperm DNA Hypomethylation Proximal to Reproduction Pathway Genes in Maturing Elite Norwegian Red Bulls

Effect ofMoringa oleiferaseed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen

Effect of Swim-Up and Glass Wool Techniques, With Adding Antioxidants to Tris Extender on Improving Post-Cryopreserved Total Sperm Characteristics in Straw and Freezability Percentage for Low Semen Quality of Holstein Bulls

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