Integron-Associated Antibiotic Resistance in
            <i>Salmonella enterica</i>
            Serovar Typhi from Asia

Ploy, Marie-Cécile; Chainier, Delphine; Thi, Nhu Hoa Tran; Poîlane, I.; Cruaud, P.; Denis, François; Collignon, Anne; Lambert, Thierry

doi:10.1128/aac.47.4.1427-1429.2003

Cited by 40 publications

(38 citation statements)

References 24 publications

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“…In this study, all MDR S. Typhi strains harbored a class 1 integron carrying the dfrA7 gene cassette, conferring resistance to trimethoprim, which is similar to the results of earlier studies on other S. Typhi isolates from different countries of South East Asia [8,15]. The presence of the integron must not be undervalued since two reports have already described multidrug-resistant S. Typhi strains harboring integrons with up to six drug resistance genes [34,35]. Furthermore, integrons can acquire additional resistance gene cassettes such as veb-1 or blaVIM, leading to serious problems in the management of these infections.…”

Section: Discussionsupporting

confidence: 88%

Emergence of plasmid-mediated multidrug resistance in epidemic and non-epidemic strains of Salmonella enterica serotype Typhi from Jordan

Alsanouri

Paglietti

Haddadin

et al. 2008

J Infect Dev Ctries

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Background: Enteric fever caused by Salmonella enterica serovar Typhi has not been adequately explored in Jordan. Methodology: In this study we investigated antibiotic resistance patterns and resistance determinants coupled with fingerprint methods of forty-eight isolates of S. Typhi obtained from 113 patients with suspected enteric fever admitted at six governmental hospitals in different directorates in Jordan. Twenty-four isolates were from an outbreak of typhoid fever that occurred between October 2004 and January 2005, and another twenty-four were from sporadic cases from 2005. Results: All isolates of S. Typhi were resistant to streptomycin. A multidrug resistant (MDR) pattern of ampicillin, chloramphenicol, co-trimoxazole with tetracycline and streptomycin (R-type ACCoTS) was found in 58% of the epidemic strains causing the outbreak and in 98% of the strains from sporadic cases. MDR isolates harbored a single IncHI1 plasmid containing a class 1 integron (dfrA7). Plasmid conjugation studies demonstrated a genetic transfer of resistance (ACCoT). S. Typhi isolates were all sensitive to fluoroquinolones and cefotaxime, the alternative drugs recommended for treatment of typhoid fever. The genomic analysis using PFGE showed: a) the outbreak was caused by an introduced circulating clone with/without an MDR plasmid, and b) isolates from the sporadic cases from 2005 are the same MDR clone that persisted and spread in the country. Conclusion: The emergence of MDR S. Typhi strains is a majorn important public health issue in Jordan. This study should guide selection of effective antibiotic therapy for the treatment of typhoid and monitoring of the spread of MDR of S. Typhi.

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Section: Discussionsupporting

confidence: 88%

Emergence of plasmid-mediated multidrug resistance in epidemic and non-epidemic strains of Salmonella enterica serotype Typhi from Jordan

Alsanouri

Paglietti

Haddadin

et al. 2008

J Infect Dev Ctries

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“…Since the first identification in an Serratia marcescens strain isolated in Japan in 1993 and the second report associated with blaGES-1 in Klebsiella pneumoniae strain FFUL 22K, up to date, class 3 integron has only been described in Acinetobacter spp., Alcaligenes, Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas putida, Salmonella spp and Serratia marcescens (Arakawa et al, 1995;Correia et al, 2003;Nemergut, 2008;Segal, 1999;Naohiro Shibata, 2003;Ploy et al, 2003). Class 3 integron had been commonly reported with low incidence and mediating IMP-1 Metallo-Beta-Lactamase (Arakawa et al, 1995, Shibata, 1999.…”

Section: Class 3 Integronmentioning

confidence: 99%

Role of integrons in antimicrobial resistance: A review

Gao¹,

Li²,

Liu³

et al. 2013

Afr. J. Microbiol. Res.

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Antibiotic resistance in bacteria still remains one of the leading concerns in global public health. Horizontal transfer of resistance genes is considered to be a major cause to facilitate the rapid spread of antibiotic resistance in microbes. In recent years, the role of integrons as mobile genetic elements playing a central role in horizontal transfer of antibiotic resistance have been well studied and documented. Nevertheless, up to date, most of the available investigations and studies on integron had focused on class 1 integrons of Gram-negative microorganisms. In the last decade, class 4 integrons (also known as super integrons) has been considered a major concern in antimicrobial resistance, as well as, a significant factor in bacterial genome evolution; however, most reports had been limited to Vibrio. Thus, this review is aimed at summarizing the occurrence and prevalence of integrons in bacterial isolates, with the focus on class 1 integron within Gram-positive bacteria, class 2 and 3 integrons.

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“…Moreover, the dfrVII gene cassette was found in S. typhi associated with an incompatibility plasmid. 17 In this study, four stains (E5, E18, E21 and E27) isolated from poultry faeces and one stain (C15) isolated from patients were found to be positive for class I integron ( Figure 3) conferring resistance to common antibiotics such as ampicillin, cotrimoxazole, cephalexin, erythromycin, nalidixic acid and tetracycline. Stains E32, C3, C5 and C9 did not contain any plasmid and integron I but found to be different antibiotic resistance (Table 2).…”

Section: Resultsmentioning

confidence: 53%

“…The occurrence of multidrug resistance (MDR) to ampicillin, chloramphenicol, sulfonamides, streptomycin, tetracycline, and trimethoprim in Salmonella enterica serovar typhi has been increasing and MDR strains have been responsible for outbreaks on the Asian subcontinents. 17 In a multinational study in Denmark, Germany, Italy, Spain and the United States and another study in France, thus confirmed the spread and persistence of this multidrug-resistant for epidemic strain of S. enterica serotype typhimurium. 18,19 Six out of ten strains contained plasmids ranging from 140-62 MDa and the result has been shown in 20 Class I integrons are the most extensively studied integrons, which are thought to be a major factor implicated in the dissemination of antibiotic resistance.…”

Section: Resultsmentioning

confidence: 71%

Antibiotic Resistance, Plasmids and Integron Profile of Salmonella Species Isolated from Poultry Farm and Patients

Begum

Mannan

Ahmed

2017

Dhaka Univ. J. Pharm. Sci

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A total of ten selected strains of Salmonella species, five from the environment of poultry farm and five from patients were included in this study. All strains were isolated and identified by using selective media, standard biochemical and serological tests. Antimicrobial susceptibility tests were performed by disc diffusion method using twelve commercial antibiotic discs of aztreonam, ceftriaxone, kanamycin, ciprofloxacin, gentamycin, chloramphenicol, ampicillin, erythromycin, cephalexin, tetracycline, cotrimoxazole and nalidixic acid. Plasmid profile and integron gene detection were conducted by Kado-Liu method and PCR, respectively. Only aztreonam, ceftriaxone, kanamycin and gentamycin were shown to be inhibitory to all strains. However, ciprofloxacin, chloramphenicol, amoxicilin, erythromycin, cephalexin, tetracycline, cotrimoxazole and nalidixic acid revealed different degrees of resistance pattern against environmental and clinical strains. Analysis of plasmid demonstrated that three environmental strains contained both plasmids of 140 Mda and 62 Mda. On the other hand, another three strains, one environmental and two clinical isolate only contained 140 Mda plasmid. All the plasmid containing strains (140 Mda and 62 Mda) exhibited same type of drug resistance pattern, whereas strains containing the 140 Mda plasmid only did not show similar type of resistance pattern. Therefore, no correlation was found between plasmid containing strains and drug resistance. Four environmental strains were also found to be positive for the class I integron and one clinical isolated was positive for class I integron gene conferring resistance to common antibiotics. However, none of the strains were found to contain class II integron. Therefore, the present study demonstrated that both environmental and clinical strains contain both large to middle size plasmids and integron I but not integron II. The plasmid and integron I containing strains experienced resistance to different antibiotics, used in the experiments.

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Integron-Associated Antibiotic Resistance in Salmonella enterica Serovar Typhi from Asia

Cited by 40 publications

References 24 publications

Emergence of plasmid-mediated multidrug resistance in epidemic and non-epidemic strains of Salmonella enterica serotype Typhi from Jordan

Emergence of plasmid-mediated multidrug resistance in epidemic and non-epidemic strains of Salmonella enterica serotype Typhi from Jordan

Role of integrons in antimicrobial resistance: A review

Antibiotic Resistance, Plasmids and Integron Profile of Salmonella Species Isolated from Poultry Farm and Patients

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