Integron content of Salmonella enterica serotype Typhimurium strains isolated in Hungary in the years 1997-1999

Gadó, I.; Pászti, Judit; Király, Margit; Jakab, Melinda

doi:10.1556/avet.51.2003.2.1

Cited by 5 publications

(11 citation statements)

References 44 publications

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“…Namely, according to Collis and Hall (1992) this primer pair sets off the amplification of the aadA2 gene only if the latter is present in a free cassette, or if the integron contains two identical cassettes of tandem arrangement, as from these primers the synthesis starts in divergent directions. At the same time, in the present case there was no reason to suppose either of the above two conditions, the more so as the integrons of DT104, unlike the non-DT104 strains, showed striking stability (Gadó et al, 2003), and thus the presence of several free cassettes could not be expected. This contradiction was explained by the sequence of the RH50/RH51 amplicon.…”

Section: Discussioncontrasting

confidence: 45%

“…The results were visualised by staining with 0.5 µg/ml ethidium bromide and evaluated over UV transillumination applying GelDoc (BioRad) with Quantity One software. (Gadó et al, 2003); IP1: 1, IP2: 1.2, IP3: 1+1.2, IP4: (0.64)+2.05, IP5: 1+(2.2); IP6: 1.9, IP9: (0.7)+ 1.45+2.05+ (3.5) kb integrons; In parentheses: weak, unstable bands; RDNC = This culture reacts with the typing phages but does not conform to a recognised type; UNTY = untypable …”

Section: Pcrmentioning

confidence: 99%

“…For the majority of the other, non-DT104 S. Typhimurium strains it was associated with an integron of 2.05 kb size (Gadó et al, 2003).…”

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confidence: 99%

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Analysis of gene cassettes of streptomycin-spectinomycin resistance of Hungarian Salmonella enterica serotype Typhimurium strains

Nógrády

Gadó

Pászti

et al. 2003

Acta Veterinaria Hungarica

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By PCR using the ant(3")-Ia primer pair the aadA gene was detected in 34 streptomycin-and spectinomycin-resistant Salmonella enterica serotype Typhimurium strains. Out of them 12 belonged to DT104 and 22 to non-DT104 phage type. Using different primer combinations it was demonstrated that this gene was integron-associated in all cases: in the DT104 strains it was generally contained by a 1 kb integron while in the majority of the non-DT104 strains by a 2.05 kb (less often by a 1.9 or 1 kb) integron. In the case of integrons carrying multiple cassettes the cassette containing the aadA gene was located closer to the 3' end of the integron. The aadA genes of DT104 and non-DT104 strains were different: in the former group the aadA2 gene, while in the latter group (constituted by strains of five different phages types as well as unclassifiable and untypable strains) the aadA1 gene could be identified. The RH50/RH51 primer pair described by Collis and Hall (1992) proved to be suitable for rapid discrimination between the aadA1 and aadA2 genes on the basis that the RH51 primer bound exclusively to the aadA2 gene.Key words: Salmonella Typhimurium, streptomycin/spectinomycin resistance, aadA gene cassettes, sequencing Salmonella enterica serotype Typhimurium (S. Typhimurium) phage type DT104 has great epidemiological importance in Europe and in the United States (Threlfall et al., 1994;Low et al., 1997;Besser et al., 1997) and is usually characterised by the high prevalence of multidrug resistance to ampicillin (Amp), chloramphenicol (Chl), streptomycin (Sm), spectinomycin (Spc), sulphonamides (Su) and tetracycline (T) (Threlfall et al., 1996;Glynn et al., 1998;Ridley and Threlfall, 1998 Acta Veterinaria Hungarica 51, 2003 it had become the dominant phage type already by 1991 (Szmollény et al., 2000;Pászti et al., 2001). Within the DT104 phage type, only in Felix and Callow's (1951) phage type 2 group did the incidence of multiresistant strains substantially exceed the average level, while in the phage type 2c group it did not (Pászti et al., 2001).The aadA gene responsible for Sm/Spc resistance is the commonest integron-associated gene among isolates of Enterobacteriaceae in Europe (MartinezFreijo et al., 1999). Its variants constitute a gene family the two most important members of which are aadA1 sequenced by Hollingshead and Vapnek (1985), Fling et al. (1985) and Sundström et al. (1988), and aadA2 sequenced by Tait et al. (1985) and Bito and Susani (1994). Further variants (aadA3-A8, aadAsc) have been described from E. coli, Pseudomonas aeruginosa and Salmonella choleraesuis isolates (Leung et al., 1992;Naas et al., 1999;Adrian et al., 2000;Mazel et al., 2000;White et al., 2000;Peters et al., 2001). The integron nature of the aadA gene and the plasmid-and/or transposon-associated location of the integrons may have played a role in the excessive horizontal spread of aadA.In both of the above two groups of the DT104 strains analysed in this study, Sm/Spc resistance was closely associated with the presence of a 1 k...

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Section: Discussioncontrasting

confidence: 45%

Section: Pcrmentioning

confidence: 99%

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Analysis of gene cassettes of streptomycin-spectinomycin resistance of Hungarian Salmonella enterica serotype Typhimurium strains

Nógrády

Gadó

Pászti

et al. 2003

Acta Veterinaria Hungarica

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“…The appearance and spread of penta-resistant Salmonella Typhimurium definitive phage type 104 (DT 104) strains of human and animal origin in Hungary has been reported recently [6,7]. Preliminary data on the relationship of antibiotic resistance and integron carriage of S. Typhimurium strains have also been published on a collection of 105 strains isolated in 1998 [8,9]. However, there are no data at all on the antibiotic resistance and integron carriage of the other commonly isolated human Salmonella serotypes such as S. Enteritidis, S. Infantis, S. Hadar and S. Virchow from Hungary.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance and class 1 integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003

Nógrády

Gadó

Tóth

et al. 2005

International Journal of Antimicrobial Agents

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“…PCR reactions were conducted essentially as described earlier (Gado et al, 2003) using primers and PCR conditions specific for catA (Guerra et al, 2001), flo (Bolton et al, 1999), cmlA (Guerra et al, 2001), or class 1 integron (Levesque et al, 1995). The amplicons obtained were detected by horizontal agarose gel electrophoresis in 2% agarose gels, stained in ethidium-bromide solution, photographed under UV and evaluated using the Bio-Rad Gel-Documentation system and the Quantity One software (Bio-Rad Laboratories, CA, USA).…”

Section: Pcr Reactions and Sequencingmentioning

confidence: 99%

Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar Typhimurium isolated from human and animal sources in Hungary

Nógrády¹,

Gadó²,

Fekete³

et al. 2005

Vet. Med. - Czech

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The presence of the chloramphenicol resistance genes cat, cmlA, flo, and the role of plasmids and class 1 integrons in the spread and persistence of chloramphenicol resistance were investigated on a collection of 40 Salmonella enterica serovar Typhimurium strains isolated from animals and humans in Hungary, by PCR and conjugation. Three groups of chloramphenicol resistant strains were identified. Eleven animal and 13 human isolates harboured the flo gene, encoding resistance to chloramphenicol and florfenicol, and possessed integrons of 1.0 Kb and 1.2 Kb typically found on the multidrug resistance island of S. Typhimurium DT104. Fifteen human strains had two different chloramphenicol resistance genes: the catB3 gene, identified as a gene cassette within a 1.45 Kb integron, and the catA gene, both of which were located on and transferred by a 140 Kb plasmid from a representative strain to the E. coli recipient via conjugation. A single animal strain had the catA gene alone, which was also transferred by a 35 Kb plasmid via conjugation. These three groups of strains belonged to three distinct genetic clusters, as it was revealed by macrorestriction analysis of 18 selected strains. This study provides information on the versatile genetic background of the chloramphenicol and florfenicol resistances in S. Typhimurium in Hungary and points to the significance of mobile genetic elements such as conjugative R-plasmids and integrons in the spread and persistence of chloramphenicol resistance genes. The results also indicate the predominance of the flo gene among animal strains and its appearance among human strains inHungary.

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Integron content of Salmonella enterica serotype Typhimurium strains isolated in Hungary in the years 1997-1999

Cited by 5 publications

References 44 publications

Analysis of gene cassettes of streptomycin-spectinomycin resistance of Hungarian Salmonella enterica serotype Typhimurium strains

Analysis of gene cassettes of streptomycin-spectinomycin resistance of Hungarian Salmonella enterica serotype Typhimurium strains

Antibiotic resistance and class 1 integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003

Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar Typhimurium isolated from human and animal sources in Hungary

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