Integron mediated bacterial resistance and virulence on clinical pathogens

Chen, Dingqiang; Jiang, Yueting; Feng, Donghua; Wen, Shuxian; Su, Danhong; Yang, Ling

doi:10.1016/j.micpath.2017.12.029

Cited by 10 publications

(6 citation statements)

References 53 publications

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“…provides the potential to transfer the resistance gene cluster (Chen et al, 2018). In addition, we speculate that P. juntendi and P. terrae may be intermediate carriers of MexCD-oprJ transmission to other P. aeruginosa or P. chromosomes; however, more experiments and data are required to support this speculation.…”

Section: Discussionmentioning

confidence: 87%

“…Using the ISfinder tool ( https://www‐is.biotoul.fr/ ), two genes encoding site‐specific integrases, which can capture and co‐transfer large DNA fragments (Chen et al., 2018 ), were located upstream of the tmexCD3‐toprJ3 gene (Figure 1 ). Meanwhile, two IS belonging to the Tn3 family ( TnAs2, TnAs3 ) and one insertion sequence belonging to the IS 1182 family (IS Pa7 ) were identified in P. juntendi , while two IS, IS Cfr1 and IS Vsa3 , were found in P. terrae originating from the IS 1182 and IS 91 families, respectively (Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…IS are abundant mobile genetic elements on bacterial genomes, responsible for the mobilisation of many antimicrobial resistance genes (Razavi et al., 2020 ). The IS close to tmexCD3‐toprJ3 provides the potential to transfer the resistance gene cluster (Chen et al., 2018 ). In addition, we speculate that P. juntendi and P. terrae may be intermediate carriers of MexCD‐oprJ transmission to other P. aeruginosa or P .…”

Section: Discussionmentioning

confidence: 99%

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Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China

Yang

Zeng

et al. 2022

Veterinary Medicine & Sci

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Background: Tigecycline is regarded as a last-resort antimicrobial agent against multidrug resistance (MDR). However, resistance-nodulation-division efflux pump resistance genes, such as tmexCD3-toprJ3, that confer resistance to tigecycline have emerged.Objectives: This study aimed to characterise the tmexCD3-toprJ3 gene cluster in Pseudomonas juntendi and Proteus terrae isolated from a pig farm.Methods: Samples were obtained from a Chinese piggery and included 92 pig faecal samples, 56 wastewater samples, 23 drinking water samples, 28 sow vagina samples and nine sow uterus swabs. The presence of tmexCD3-toprJ3 was detected using a polymerase chain reaction assay, and the antimicrobial susceptibility profile of the tmexCD3-toprJ3-positive isolates was determined using the broth dilution method. Genomic locations were identified using whole-genome sequencing and bioinformatics. Results:We identified two tmexCD3-toprJ3-positive isolates, and both isolates were highly resistant to antibiotics such as tigecycline. In addition, we identified several mobile elements (ISPa7, ISCfr1, ISVsa3) and insertion sequences (TnAs2, TnAs3) in tmexCD3-toprJ3-positive isolates that could increase the potential for the spread of MDR. Conclusions:To the best of our knowledge, this study is the first to report the detection of tmexCD3-toprJ3 in P. juntendi and P. terrae isolated from a pig farm.

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Section: Discussionmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China

Yang

Zeng

et al. 2022

Veterinary Medicine & Sci

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“…IS elements can help resistance genes to transfer between and within bacteria [36] and can upregulate downstream resistance genes [37]. Integrons are another MGE responsible for the emergence and spread of antibiotic resistance genes, including β-lactamases, aminoglycosides, and fluoroquinolones [38]. Transposons, like plasmids, have the potential to transfer horizontally or vertically among pathogens, driving the development of antibiotic resistance [39].…”

Section: Resultsmentioning

confidence: 99%

Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes

et al. 2019

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Background Multidrug resistance is a growing global public health threat with far more serious consequences than generally anticipated. In this study, we investigated the antibiotic resistance and genomic traits of a clinical strain of Escherichia coli LCT-EC001. Results LCT-EC001 was resistant to 16 kinds of widely used antibiotics, including fourth-generation cephalosporins and carbapenems. In total, up to 68 determinants associated with antibiotic resistance were identified, including 8 beta-lactamase genes (notably producing ESBLs and KPCs), 31 multidrug efflux system genes, 6 outer membrane transport system genes, 4 aminoglycoside-modifying enzyme genes, 10 two-component regulatory system genes, and 9 other enzyme or transcriptional regulator genes, covering nearly all known drug-resistance mechanisms in E. coli . More than half of the resistance genes were located close to mobile genetic elements, such as plasmids, transposons, genomics islands, and insertion sequences. Phylogenetic analysis revealed that this strain may have evolved from E. coli K-12 but is a completely new MLST type. Conclusions Antibiotic resistance was extremely severe in E. coli LCT-EC001, mainly due to mobile genetic elements that allowed the gain of a large quantity of resistance genes. The antibiotic resistance genes of E. coli LCT-EC001 can probably be transferred to other bacteria. To the best of our knowledge, this is the first report of a strain of E. coli which has such a large amount of antibiotic resistance genes. Apart from providing an E. coli reference genome with an extremely high multidrug-resistant background for future analyses, this work also offers a strategy for investigating the complement and characteristics of genes contributing to drug resistance at the whole-genome level. Electronic supplementary material The online version of this article (10.1186/s13099-019-0298-5) contains supplementary material, which is available to authorized users.

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“…Integrons contain specific recombinant sites, which can propagate antibiotic resistance, enable transmission of different drug resistance gene cassettes from bacterial drug resistance genes among different strains and lead to multidrug-resistance among bacteria ( 18 ). The most common integron type of ABA are the class I integrons, which contains various drug resistance genes and is closely associated with the acquisition of drug resistance ( 19 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular typing, and integron and associated gene cassette analyses in Acinetobacter baumannii strains isolated from clinical samples

Deng

Wang

et al. 2020

Exp Ther Med

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The present study aimed to investigate the association between drug resistance and class I, II and III integrons in Acinetobacter baumannii (ABA). Multilocus sequence typing (MLST) is a tool used to analyze the homology among house-keeping gene clusters in ABA and ABA prevalence and further provides a theoretical basis for hospitals to control ABA infections. A total of 96 clinical isolates of non-repeating ABA were harvested, including 74 carbapenem-resistant ABA (CRABA) and 22 non-CRABA strains, and used for bacterial identification and drug susceptibility analysis. Variable regions were sequenced and analyzed. Then, 7 pairs of housekeeping genes were amplified and sequenced via MLST and sequence alignment was performed against the Pub MLST database to determine sequence types (STs) strains and construct different genotypic evolutionary diagrams. The detection rate of CRABA class I integrons was 13.51% (10/74); no class II and III integrons were detected. However, class I, II and III integrons were not detected in non-CRABA strains. The variable regions of 9 of 10 class I integrons were amplified and 10 gene cassettes including aacC1, aac1, aadDA1, aadA1a, aacA4, dfrA17, aadA5, aadA1, aadA22 and aadA23 were associated with drug resistance. The 96 ABA strains were divided into 21 STs: 74 CRABA strains containing 9 STs, primarily ST208 and ST1145 and 22 non-CRABA strains containing 18 STs, primarily ST1145. Class I integrons are a critical factor underlying drug resistance in ABA. CRABA and non-CRABA strains differ significantly; the former primarily contained ST208 and ST1145, and the latter contained ST1145. Most STs were concentrated in intensive care units (ICUs) and the department of Neurology, with the patients from the ICUs being the most susceptible to bacterial infection. In the Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, ABA is potentially horizontally transmitted and MLST can be used for clinical ABA genotyping.

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Integron mediated bacterial resistance and virulence on clinical pathogens

Cited by 10 publications

References 53 publications

Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China

Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China

Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes

Molecular typing, and integron and associated gene cassette analyses in Acinetobacter baumannii strains isolated from clinical samples

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