The technology of real-time hybrid simulation (RTHS), which is one of the methods to test the performance of train operation on bridge in recent years, has strict real-time requirements. Combined with the fixed-point iteration (FPI) algorithm, this paper developed an off-line iterative hybrid simulation (OIHS) method, avoiding real-time synchronization between numerical and physical parts in RTHS to reproduce train–track–bridge coupling-vibration (TTBV) of high-speed railway in laboratory. In OIHS, the physical train model was installed on the shake table, while the response of the numerical track–bridge structure was simulated. To improve the test reproduction accuracy of the TTBV in high-speed railway, based on the shake table inherent off-line iterative control which is reproduce the commands issued to the shake table, an outer loop off-line iterative controller was designed to form a double-layer off-line iterative control framework to correct the response error between the subsystems. Compared with traditional finite element analysis, the accuracy and efficiency of the OIHS method were validated by numerical simulation and shake table test. The results demonstrated that the OIHS method can solve the dynamic convergence problem of the train–track–bridge on high-speed railway accurately and efficiently.