The quality and safety issues of fresh food have recently attracted increasing attention. At the current stage, there are still many challenges in the quality control of the fresh food supply chain, leading to frequent quality problems during the actual supply process, and the improvement of the quality of fresh food has reached a bottleneck. This paper analyses the quality control problems existing in the supply chain of fresh food in China: long supply chain, many circulation links; The technical level of fresh cold chain is backward, and the ratio of product preservation; Farmers are in a weak position, easy to produce "shoddy" moral hazard; The relationship of fresh supply chain is unstable, and the quality and safety problems are prominent. In response to these problems, the fresh food supply chain quality control strategy is proposed from the aspects of supply chain operation mode, cold chain logistics infrastructure construction, supply chain cooperation mode, fresh product quality safety and standardization construction, aiming to enhance the safety of fresh food quality and promote the development of healthy dietary practices.