“…The article highlights that conventional fluorescence-guided surgery primarily utilizes the first window (NIR-I, 700–900 nm), characterized by a limited tissue penetration depth ranging from 1 to 6 mm [ [87] , [88] , [89] ]. Conversely, the second near-infrared window (NIR-II, 1000–1700 nm) significantly surpasses the constraints imposed by tissue absorption, autofluorescence, and photon scattering, enabling profound tissue penetration (up to 20 mm), micron-scale spatial resolution, intelligent decision-making with U-Net Segmentation, and a high tumor-to-normal tissue (T/NT) ratio exceeding 190 [ [90] , [91] , [92] , [93] , [94] , [95] , [96] , [97] ]. These features hold promise for enhancing T/NT ratio and delineating tumor margins more precisely, thereby facilitating more accurate tumor resection.…”