Bamboo is a natural composite material with a high strength-to-weight ratio traditionally used in various consumer products as well as in building and modern advanced and sustainable manufacturing technology. While its macroscopic mechanical properties are well known, nanoscale studies of the mechanical properties of the hierarchical structure of bamboo at the level of individual cells and cell walls are lacking. Here we use different AFM-based methods to analyse the mechanical properties of individual bamboo fibres that are the foundation of the hierarchical structure at the nanoscale. The elastic modulus of the fiber in the outer region varies from 7.5 to 8.9 GPa, while that of the inner fiber ranges between 4.1 and 6.1 GPa. The results demonstrate that the fibre stiffness varies in nanoscale regions between fibre interior and the fibre wall and strongly depends on the position of the individual bamboo fibre within the culm. Outer fibres with high cellulose fibril density and low lignin level show low adhesion interaction force (13.5%) in the interface between cellulose and lignin/hemicellulose matrix, hence, resulting in low plastic deformation energy release during mechanical deformation. The implications of the interrelation of plasticity, lignin level, and adhesion force are discussed for the application of bamboo as a high-performance, renewable, and sustainable material.