The research on ambient assistive technology is concerned with features humanoid agents should show in order to gain user acceptance. However, differently aged groups may have different requirements. This paper is particularly focused on agent's voice preferences among elders, young adults, and adolescents. To this aim 316 users organized in groups of 45/46 subjects of which 3 groups of elders (65+ years old), 2 of young adults (aged between 22-35 years), and 2 of adolescents (aged between 14-16 years) were recruited and administered the Virtual Agent Acceptance Questionnaire (VAAQ), after watching video-clips of mute and speaking agents, in order to test their preferences in terms of willingness to interact, pragmatic and hedonic qualities, and attractiveness, of proposed speaking and mute agents. In addition, the elders were also tested on listening only the agent's. The results suggest that voice is primary for getting elder's acceptance of virtual humanoid agents in contrast to young adults and adolescents which accept equally well either mute or speaking agents.