Intense pulse light and 5‐ALA PDT: Phototoxic effects in vitro depend on the spectral overlap with protoporphyrine IX but do not match cut‐off filter notations

Maisch, Tim; Moor, Anne C.E.; Regensburger, Johannes; Ortland, C.; Szeimies, Rolf‐Markus; Bäumler, Wolfgang

doi:10.1002/lsm.20970

Cited by 15 publications

(10 citation statements)

References 26 publications

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“…the Aktilite 128 (Galderma), BF‐Rhodo LED (Biofrontera) and Omnilux PDT (Phototherapeutics, London, UK) each with an output that matches the 630/635 nm activation peak of PpIX whilst excluding the extraneous wavelengths present in broadband sources, permitting shorter illumination times. Filtered intense pulsed lights (IPLs) have been successfully used in PDT for AK, acne and photorejuvenation although they emit different spectra, resulting in a need to derive specific protocols to achieve identical radiant exposures . Narrow spectrum light sources are associated with higher response rates, with complete patient clearance rates of 85% and 68% for nanoemulsion ALA‐PDT or MAL‐PDT, respectively, compared with 72% and 61% when broad spectrum devices were used …”

Section: Methods Of Actionmentioning

confidence: 99%

European Dermatology Forum guidelines on topical photodynamic therapy 2019 Part 1: treatment delivery and established indications – actinic keratoses, Bowen's disease and basal cell carcinomas

Morton¹,

Szeimies

Basset-Séguin

et al. 2019

Acad Dermatol Venereol

148

146

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Topical photodynamic therapy (PDT) is a widely approved therapy for actinic keratoses, Bowen's disease (squamous cell carcinoma in situ), superficial and certain thin basal cell carcinomas. Recurrence rates when standard treatment protocols are used are typically equivalent to existing therapies, although inferior to surgery for nodular basal cell carcinoma. PDT can be used both as lesional and field therapies and has the potential to delay/reduce the development of new lesions. A protocol using daylight to treat actinic keratoses is widely practised, with conventional PDT using a red light after typically a 3‐h period of occlusion employed for other superficial skin cancer indications as well as for actinic keratoses when daylight therapy is not feasible. PDT is a well‐tolerated therapy although discomfort associated with conventional protocol may require pain‐reduction measures. PDT using daylight is associated with no or minimal pain and preferred by patient. There is an emerging literature on enhancing conventional PDT protocols or combined PDT with another treatment to increase response rates. This guideline, published over two parts, considers all current approved and emerging indications for the use of topical PDT in dermatology, prepared by the PDT subgroup of the European Dermatology Forum guidelines committee. It presents consensual expert recommendations reflecting current published evidence.

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Section: Methods Of Actionmentioning

confidence: 99%

European Dermatology Forum guidelines on topical photodynamic therapy 2019 Part 1: treatment delivery and established indications – actinic keratoses, Bowen's disease and basal cell carcinomas

Morton¹,

Szeimies

Basset-Séguin

et al. 2019

Acad Dermatol Venereol

148

146

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“…These red LED sources match the 630/635‐nm activation peak of PpIX while excluding the extraneous wavelengths present in broadband lights, thus permitting shorter illumination times. Filtered intense pulsed lights (IPLs) have been successfully used in PDT for AK, although phototoxic effects may not match cut‐off filter notations provided by their manufacturers 18 . Narrow‐spectrum light sources are associated with relatively higher response rates when compared with broad‐spectrum devices, with complete patient clearance rates of 85% and 68% for BF‐200 ALA‐PDT or MAL‐PDT, respectively, compared with 72% and 61% when broad‐spectrum devices were used 9,19 …”

Section: Light Sources and Dosimetrymentioning

confidence: 99%

European guidelines for topical photodynamic therapy part 1: treatment delivery and current indications – actinic keratoses, Bowen’s disease, basal cell carcinoma

Morton¹,

Szeimies

Sidoroff

et al. 2012

Acad Dermatol Venereol

316

327

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Topical photodynamic therapy (PDT) is a widely used non-invasive treatment for certain non-melanoma skin cancers, permitting treatment of large and multiple lesions with excellent cosmesis. High efficacy is demonstrated for PDT using standardized protocols in non-hyperkeratotic actinic keratoses, Bowen's disease, superficial basal cell carcinomas (BCC) and in certain thin nodular BCC, with superiority of cosmetic outcome over conventional therapies.Recurrence rates following PDT are typically equivalent to existing therapies, although higher than surgery for nodular BCC. PDT is not recommended for invasive squamous cell carcinoma. Treatment is generally well tolerated, but tingling discomfort or pain is common during PDT. New studies identify patients most likely to experience discomfort and permit earlier adoption of pain-minimization strategies. Reduced discomfort has been observed with novel protocols including shorter photosensitizer application times and in daylight PDT for actinic keratoses.

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“…The ability of TMPyP to generate singlet oxygen was qualitatively evaluated using a frequency-doubled Nd:YAG laser (PhotonEnergy, Ottensoos, Germany), as previously described, at a wavelength of 532 nm [30,31]. Singletoxygen luminescence was detected with an IR-sensitive photomultiplier (R55-09-42, Hamamatsu Photonics Deutschlang GmbH, Herrsching, Germany) at different wavelengths from 1,200 to 1,350 nm using a monochromator (HORIBA Jobin Yvon Inc. Kyoto, Japan) in front of the multiplier generated by 50 lmol l -1 of TMPyP in H 2 O.…”

Section: Detection Of Singlet Oxygenmentioning

confidence: 99%

“…The emission spectra of the IPL were normalized to it corresponding to maxima between 750 and 850 nm as previously described [31].…”

Section: Light Source and Irradiation Parametersmentioning

confidence: 99%

Fast and effective: intense pulse light photodynamic inactivation of bacteria

Maisch

Spannberger

Regensburger

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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The goal of this study was to investigate the photodynamic toxicity of TMPyP (5, 10, 15, 20-Tetrakis (1-methylpyridinium-4-yl)-porphyrin tetra p-toluenesulfonate) in combination with short pulses (ms) of an intense pulse light source within 10 s against Bacillus atrophaeus, Staphylococcus aureus, Methicillin-resistant S. aureus and Escherichia coli, major pathogens in food industry and in health care, respectively. Bacteria were incubated with a photoactive dye (TMPyP) that is subsequently irradiated with visible light flashes of 100 ms to induce oxidative damage immediately by generation of reactive oxygen species like singlet oxygen. A photodynamic killing efficacy of up to 6 log(10) (>99.9999%) was achieved within a total treatment time of 10 s using a concentration range of 1-100 μmol TMPyP and multiple light flashes of 100 ms (from 20 J cm(-2) up to 80 J cm(-2)). Both incubation of bacteria with TMPyP alone or application of light flashes only did not have any negative effect on bacteria survival. Here we could demonstrate for the first time that the combination of TMPyP as the respective photosensitizer and a light flash of 100 ms of an intense pulsed light source is enough to generate sufficient amounts of reactive oxygen species to kill these pathogens within a few seconds. Increasing antibiotic resistance requires fast and efficient new approaches to kill bacteria, therefore the photodynamic process seems to be a promising tool for disinfection of horizontal surfaces in industry and clinical purposes where savings in time is a critical point to achieve efficient inactivation of microorganisms.

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Intense pulse light and 5‐ALA PDT: Phototoxic effects in vitro depend on the spectral overlap with protoporphyrine IX but do not match cut‐off filter notations

Cited by 15 publications

References 26 publications

European Dermatology Forum guidelines on topical photodynamic therapy 2019 Part 1: treatment delivery and established indications – actinic keratoses, Bowen's disease and basal cell carcinomas

European Dermatology Forum guidelines on topical photodynamic therapy 2019 Part 1: treatment delivery and established indications – actinic keratoses, Bowen's disease and basal cell carcinomas

European guidelines for topical photodynamic therapy part 1: treatment delivery and current indications – actinic keratoses, Bowen’s disease, basal cell carcinoma

Fast and effective: intense pulse light photodynamic inactivation of bacteria

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