Intensification of lipase performance in a transesterification reaction by immobilization on CaCO3 powder

Rosu, Roxana; Iwasaki, Yoshinobu; Shimizu, Nobuhiro; Doisaki, Nobushige; Yamané, Tsuneo

doi:10.1016/s0168-1656(98)00156-4

Cited by 28 publications

(15 citation statements)

References 9 publications

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“…Because of the current high cost of some available commercial support matrixes, the possibility of using inexpensive supports for lipase immobilization such as rice husk [108], CaCO 3 powder [109], grafted hydrogels [110][111][112][113] and activated silica/celite [114] has also been considered (Table III). Although adsorption seems to be a promising technique for lipase recovery, the adsorbents are either expensive or not easily accessible.…”

Section: Lipase Modifications In Organic Solventsmentioning

confidence: 99%

Microbial lipases: At the interface of aqueous and non-aqueous media

Verma

Azmi²,

Kanwar³

2008

Acta Microbiologica et Immunologica Hungarica

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In recent times, biotechnological applications of microbial lipases in synthesis of many organic molecules have rapidly increased in non-aqueous media. Microbial lipases are the 'working horses' in biocatalysis and have been extensively studied when their exceptionally high stability in non-aqueous media has been discovered. Stability of lipases in organic solvents makes them commercially feasibile in the enzymatic esterification reactions. Their stability is affected by temperature, reaction medium, water concentration and by the biocatalyst's preparation. An optimization process for ester synthesis from pilot scale to industrial scale in the reaction medium is discussed. The water released during the esterification process can be controlled over a wide range and has a profound effect on the activity of the lipases. Approaches to lipase catalysis like protein engineering, directed evolution and metagenome approach were studied. This review reports the recent development in the field ofnon-aqueous microbial lipase catalysis and factors controlling the esterification/transesterification processes in organic media.

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Section: Lipase Modifications In Organic Solventsmentioning

confidence: 99%

Microbial lipases: At the interface of aqueous and non-aqueous media

Verma

Azmi²,

Kanwar³

2008

Acta Microbiologica et Immunologica Hungarica

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“…The high cost of popular supports (silica-based carriers and synthetic polymers) causes many to search for a cheaper substitute such as CaCO 3 (Rosu et al, 1998), rice husk and rice straw (Tantrakulsiri et al, 1997) or chitin and chitosan (Krajewska, 1991;Felse and Panda, 1999). Of these alternatives, the derivative of chitin, chitosan, appears to be the most attractive since chitin is the second the most abundant biopolymer in nature next to cellulose (Krajewska, 1991).…”

Section: Introductionmentioning

confidence: 99%

Immobilization and catalytic properties of lipase on chitosan for hydrolysis and esterification reactions

Pereira

Zanin

Castro

2003

Braz. J. Chem. Eng.

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-The objective of this study was to evaluate the immobilization of lipase on a chitosan support by physical adsorption, aiming at its application in hydrolytic and synthetic reactions. Two types of chitosan (flakes and porous) were used for immobilizing lipase from a microbial source (Candida rugosa) and animal cells (porcine pancreas). The best results for recovery of total activity after immobilization were obtained for microbial lipase and porous chitosan beads. This set was selected for further immobilization studies, including full characterization of the immobilized derivative in aqueous and organic media. In aqueous medium, the operational and thermal stabilities of this preparation were quantified. In organic medium, the direct synthesis of n-butyl butyrate in organic solvent was chosen as a model reaction. The influence of several parameters, such as temperature, initial butyric acid concentration and amount of enzyme in the reaction system, was analyzed. Production of n-butyl butyrate was optimized and an ester yield response equation was obtained, making it possible to predict ester yields from known values of the three main factors. Use of this immobilized preparation was extended to the direct esterification of a large range of carboxylic acids (from C 2 to C 12 ) with a variety of alcohols (from C 2 to C 10 ).

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“…In this regard, a variety of metal nanoparticle/carbon nanotube hybrid nanostructures can be applied as sensitive modified electrodes [103]. Calcium carbonate (CaCO 3 ), a natural mineral with good biocompatibility, has been shown to improve enzyme efficiency [104]. Spherical polymorph is supposed to be used for a variety of purposes owing to its specific characteristics, such as larger surface area better water dispersity and lower specific gravity than the other crystal models [105,106].…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

2020

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Background: The electrochemical biosensor is one of the typical sensing devices based on transducing the biochemical events to electrical signals. In this type of sensor, an electrode is a key component that is employed as a solid support for immobilization of biomolecules and electron movement. Thanks to numerous nanomaterials that possess the large surface area, synergic effects are enabled by improving loading capacity and the mass transport of reactants for achieving high performance in terms of analytical sensitivity. Main body: We categorized the current electrochemical biosensors into two groups, carbon-based (carbon nanotubes and graphene) and non-carbon-based nanomaterials (metallic and silica nanoparticles, nanowire, and indium tin oxide, organic materials). The carbon allotropes can be employed as an electrode and supporting scaffolds due to their large active surface area as well as an effective electron transfer rate. We also discussed the non-carbon nanomaterials that are used as alternative supporting components of the electrode for improving the electrochemical properties of biosensors. Conclusion: Although several functional nanomaterials have provided the innovative solid substrate for high performances, developing on-site version of biosensor that meets enough sensitivity along with high reproducibility still remains a challenge. In particular, the matrix interference from real samples which seriously affects the biomolecular interaction still remains the most critical issues that need to be solved for practical aspect in the electrochemical biosensor. Recently, various electrochemistry-driven biosensing methods have been introduced for simple and miniaturized analytical devices for on-site analysis. This trend can be applied to replace the commercial lab instruments manufactured by the renowned in vitro diagnosis

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Intensification of lipase performance in a transesterification reaction by immobilization on CaCO3 powder

Cited by 28 publications

References 9 publications

Microbial lipases: At the interface of aqueous and non-aqueous media

Microbial lipases: At the interface of aqueous and non-aqueous media

Immobilization and catalytic properties of lipase on chitosan for hydrolysis and esterification reactions

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

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