Intensifying the Anticancer Potential of Cationic Peptide Derived from Serine Threonine Protein Kinase of Teleost by Tagging with Oligo Tryptophan

Prabha, Nagaram; Sannasimuthu, Anbazahan; Kumaresan, Venkatesh; Elumalai, Preetham; Arockiaraj, Jesu

doi:10.1007/s10989-019-09817-3

Cited by 18 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Damages in the cell membrane clearly affects cellular morphology. Abnormal morphology indicates apoptosis, such as granulation cell shrinkage [ 26 ]. PS14-treated cells showed loss of cell structure, granulation and shrinkage ( Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans, on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

et al. 2022

Self Cite

View full text Add to dashboard Cite

In this study, the anti-cancer and anti-inflammatory activities of PS14, a short peptide derived from the cellulase binding domain of pathogenic fungus, Aphanomyces invadans, have been evaluated, in vitro and in vivo. Bioinformatics analysis of PS14 revealed the physicochemical properties and the web-based predictions, which indicate that PS14 is non-toxic, and it has the potential to elicit anti-cancer and anti-inflammatory activities. These in silico results were experimentally validated through in vitro (L6 or Hep-2 cells) and in vivo (zebrafish embryo or larvae) models. Experimental results showed that PS14 is non-toxic in L6 cells and the zebrafish embryo, and it elicits an antitumor effect Hep-2 cells and zebrafish embryos. Anticancer activity assays, in terms of MTT, trypan blue and LDH assays, showed a dose-dependent inhibitory effect on cell proliferation. Moreover, in the epithelial cancer cells and zebrafish embryos, the peptide challenge (i) caused significant changes in the cytomorphology and induced apoptosis; (ii) triggered ROS generation; and (iii) showed a significant up-regulation of anti-cancer genes including BAX, Caspase 3, Caspase 9 and down-regulation of Bcl-2, in vitro. The anti-inflammatory activity of PS14 was observed in the cell-free in vitro assays for the inhibition of proteinase and lipoxygenase, and heat-induced hemolysis and hypotonicity-induced hemolysis. Together, this study has identified that PS14 has anti-cancer and anti-inflammatory activities, while being non-toxic, in vitro and in vivo. Future experiments can focus on the clinical or pharmacodynamics aspects of PS14.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Similar to this outcome, serine-threonine protein kinase protein of Channa striatus derived peptide IW13 showed anti-cancer activity against A549, MCF-7 and Hela cells. Moreover, IW13 significantly altered the cellular morphology [ 26 ]. Different apoptotic cell stages were identified by nuclear staining (Hoechst 33342).…”

Section: Resultsmentioning

confidence: 99%

Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans, on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cytotoxic effect on MCF-7 or MDA-MB-231 cancer cell lines has been reported for the cationic peptides Bovine lactoferricin [84], its Bovine variant lactoferricin 6 [85], pBmje [86], Magainin II [87], the Lysine-substituted VmCT1 analogs [88], IW13 [89], Peptoide 1 [90],…”

Section: Anticancer Activities Of Bioactive Cationic Peptidesmentioning

confidence: 99%

Bioactive Cationic Peptides as Potential Agents for Breast Cancer Treatment

2021

View full text Add to dashboard Cite

Breast cancer continues to affect millions of women worldwide, and the number of new cases dramatically increases every year. The physiological causes behind the disease are still not fully understood. One in every 100 cases can occur in men, and although the frequency is lower than among women, men tend to have a worse prognosis of the disease. Various therapeutic alternatives to combat the disease are available. These depend on the type and progress of the disease, and include chemotherapy, radiotherapy, surgery, and cancer immunotherapy. However, there are several well-reported side effects of these treatments that have a significant impact on life quality, and patients either relapse or are refractory to treatment. This makes it necessary to develop new therapeutic strategies. One promising initiative are bioactive peptides, which have emerged in recent years as a family of compounds with an enormous number of clinical applications due to their broad spectrum of activity. They are widely distributed in several organisms as part of their immune system. The antitumoral activity of these peptides lies in a nonspecific mechanism of action associated with their interaction with cancer cell membranes, inducing, through several routes, bilayer destabilization and cell death. This review provides an overview of the literature on the evaluation of cationic peptides as potential agents against breast cancer under different study phases. First, physicochemical characteristics such as the primary structure and charge are presented. Secondly, information about dosage, the experimental model used, and the mechanism of action proposed for the peptides are discussed.

show abstract

“…There are five AMP families in fish: (a) hepcidins are a major part of innate immunity with anticancer, antibacterial, and antiviral properties (Wang et al, 2010), (b) defensins are another innate component and they are conserved among animals and plants, (c) histone‐derived peptides have multifarious pathogenic activities (Masso‐Silva & Diamond, 2014; Prabha et al, 2020), (d) cathelicidins were suggested to be a major part of both innate and adaptive immunity (Maier et al, 2008), they have antimicrobial activity with no cytotoxicity (Lu et al, 2011) and have identical genetic structure in both fish and mammalia, which indicates that mammalian cathelicidins originate from fish (Patrzykat & Hancock, 2019), and (e) piscidins exhibit antibacterial, antifungal, and antiviral properties with an innate immune response against parasitic infections (Capillo et al, 2021; Raju et al, 2021).…”

Section: Osteichthyesmentioning

confidence: 99%

Comparison of vertebrate skin structure at class level: A review

Akat

Yenmiş

Pombal

et al. 2022

The Anatomical Record

View full text Add to dashboard Cite

The skin is a barrier between the internal and external environment of an organism. Depending on the species, it participates in multiple functions. The skin is the organ that holds the body together, covers and protects it, and provides communication with its environment. It is also the body's primary line of defense, especially for anamniotes. All vertebrates have multilayered skin composed of three main layers: the epidermis, the dermis, and the hypodermis. The vital mission of the integument in aquatic vertebrates is mucus secretion. Cornification began in apmhibians, improved in reptilians, and endured in avian and mammalian epidermis. The feather, the most ostentatious and functional structure of avian skin, evolved in the Mesozoic period. After the extinction of the dinosaurs, birds continued to diversify, followed by the enlargement, expansion, and diversification of mammals, which brings us to the most complicated skin organization of mammals with differing glands, cells, physiological pathways, and the evolution of hair. Throughout these radical changes, some features were preserved among classes such as basic dermal structure, pigment cell types, basic coloration genetics, and similar sensory features, which enable us to track the evolutionary path. The structural and physiological properties of the skin in all classes of vertebrates are presented. The purpose of this review is to go all the way back to the agnathans and follow the path step by step up to mammals to provide a comparative large and updated survey about vertebrate skin in terms of morphology, physiology, genetics, ecology, and immunology.

show abstract

Intensifying the Anticancer Potential of Cationic Peptide Derived from Serine Threonine Protein Kinase of Teleost by Tagging with Oligo Tryptophan

Cited by 18 publications

References 27 publications

Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans, on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans, on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

Bioactive Cationic Peptides as Potential Agents for Breast Cancer Treatment

Comparison of vertebrate skin structure at class level: A review

Contact Info

Product

Resources

About