Intensity‐specific effect of physical activity on urinary levels of 8‐hydroxydeoxyguanosine in middle‐aged Japanese

Abstract

Physical activity (PA) is recommended to both promote and maintain health and prevent cancer by improving the body's DNA repair system, which is considered a mechanism of cancer prevention. However, associations between PA and urinary levels of 8‐hydroxydeoxyguanosine (8‐OH‐dG), which reflects DNA damage, are unclear. This cross‐sectional study included 2370 men and 4052 women aged 45–74 years enrolled between 2010 and 2012. Habitual PA was assessed by single‐axis accelerometer and urinary 8‐OH‐dG levels by automated HPLC. Multiple linear regression analysis was used to examine the relationship between log‐transformed urinary 8‐OH‐dG and total PA (TPA) and PA of moderate/vigorous intensity (MVPA; ≥3 metabolic equivalents), with adjustment for age, body mass index, energy intake, alcohol consumption, smoking status, daily coffee drinking, menopause status (in women), and TPA (for MVPA). On multivariate adjustment, urinary 8‐OH‐dG levels were inversely correlated with TPA (β = −0.020, P < 0.01) in women, and this correlation was not changed by PA intensity. Conversely, urinary 8‐OH‐dG levels were inversely correlated with MVPA (β = −0.022, P < 0.05) in men, although the correlation with TPA was non‐significant. This inverse correlation was clearer in current smokers than in never or former smokers, although the interaction between smoking status and MVPA on urinary 8‐OH‐dG levels was non‐significant. In conclusion, greater TPA in women and greater MVPA in men were correlated with reduction in urinary 8‐OH‐dG, suggesting sex‐specific effects of MVPA and TPA on protection from oxidative DNA damage. Increasing PA may mediate reduction in oxidative stress.

Salivary 8-hydroxyguanine as a lifestyle-related oxidative stress biomarker in workers

“…Moderate physical activity also reduced oxidative stress. (37,38) At the same time, GSH (Glutathione-SH), catalase, glutathione peroxidase activity, (2,39) and total antioxidant capacity (38) were increased. The salivary 8-OHGua levels were significantly lower in persons who performed moderate physical activity.…”

Leisure-time physical activity and DNA damage among Japanese workers

“…In a study of male Japanese workers, total physical activity—including commuting, working, and sports—was inversely associated with urinary 8-OH-dG levels, independent of other lifestyle factors [17]; furthermore, moderate leisure-time exercise (less than 5 hours per week) was associated with lower concentrations of urinary 8-OH-dG [16]. In a substudy of the Japan Multi-institutional Collaborative Cohort Study, which assessed daily physical activity by using an accelerometer, showed that light-to-vigorous physical activity in women and moderate-to-vigorous physical activity in men were inversely associated with the concentration of urinary 8-OH-dG [15]. In an intervention study among patients with type 2 diabetes, aerobic exercise greatly reduced urinary 8-OH-dG levels [30].…”

“…Several epidemiological studies have examined the association between physical activity and 8-OH-dG levels, but their results have been inconsistent; some reported an inverse association between physical activity and 8-OH-dG [15–17], while others found no obvious association [18–20]. To the best of our knowledge, only one study examined the association between total physical activity and m 7 Gua, but failed to detect any significant associations [17].…”

Moderate-to-vigorous Physical Activity and Sedentary Behavior Are Independently Associated With Renal Function: A Cross-sectional Study

“…However, physical activity has been shown to reduce inflammation and oxidative stress in both the general population and patients with CKD 11 . We previously reported the presence of an inverse correlation between daily physical activity and levels of circulating inflammatory cytokines including interleukins 40 and urine 8-hydroxydeoxyguanosine, as an index of whole-body oxidative stress 41 . Further, while prolonged SB markedly reduces micro-and macrovascular dilator function 42 , these impairments can be improved with walking 43 .…”