BackgroundImproving hand hygiene is the most efficient method to prevent healthcare-associated infections, which are a severe threat to patient safety. Besides healthcare professionals, it is mainly hospital patients and visitors who pose a risk for the transmission of pathogens, which can lead to infections. In contrast to medical staff, laypeople’s hand hygiene behavior in hospitals is not well researched. Therefore, the present study had three aims: 1) Identifying a suitable theoretical model to explain laypeople's hand hygiene practice; 2) Finding the factors that strongly affect patients’ and visitors’ hand hygiene behavior and, therefore, should be targeted in behavior change interventions; and 3) Comparing the essential determinants of hand hygiene behavior between laypeople and healthcare professionals.MethodsTo accomplish these goals, in total N = 1,605 patients and visitors were surveyed on their hand hygiene practice in hospitals. The employed questionnaires were based on three theoretical models: a) the Theory of Planned Behavior (TPB); b) the Health Action Process Approach (HAPA); and c) the Theoretical Domains Framework (TDF). Structural equation modeling (SEM) was used to analyze the data.ResultsAmong patients, 53% of the variance in the hand hygiene behavior was accounted for by the TDF domains, 44% by a modified HAPA model, and 40% by the TPB factors. Among visitors, these figures were 60%, 37%, and 55%, respectively. Two clusters of variables surfaced as being essential determinants of the behavior: First, self-regulatory processes (perceived behavioral control; action control; memory, attention, and decision processes); and second, social influence processes (subjective norm and role and identity).ConclusionsThe TDF was identified as the most suitable model to investigate and predict patient’s and visitor’s hand hygiene practices. The critical determinants for adequate hand hygiene (i.e., self-regulation processes and social influence processes) are similar for laypeople and healthcare professionals. Therefore, analogous intervention strategies might be useful for both target groups. Further practical and theoretical implications are discussed.