Intensive formation of coccoid forms as a feature strongly associated with highly pathogenic Helicobacter pylori strains

Krzyżek, Paweł; Biernat, Monika; Gościniak, Grażyna

doi:10.1007/s12223-018-0665-5

Cited by 15 publications

(24 citation statements)

References 45 publications

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“…Morphological variability in response to unfavorable environmental conditions is typical for many Gram-negative rods, including H. pylori [38]. This microorganism, in response to stressful conditions, most often undergoes a morphological transformation into spherical forms, for which increased survivability and participation in the failure of antimicrobial therapies are suggested [39,40,41]. In many studies defining the antibacterial activity of substances, the mechanism of H. pylori morphological conversion from the spiral to coccoid form was noticed [42,43,44].…”

Section: Discussionmentioning

confidence: 99%

“…The strains were categorized as susceptible or resistant to antibiotics based on the EUCAST recommendations, i.e., amoxicillin (AMX, R > 0.125 μg/mL), clarithromycin (CLR, R > 0.5 μg/mL), tetracycline (TET, R > 1 μg/mL), and metronidazole (MTZ, > 8 μg/mL) [52]. Bacterial strains were kept in a Trypticase soy broth (TSB) (Oxoid, Le Pont de Claix, France) with the addition of 15% glycerol at −70 °C until testing [41]. After thawing, the bacteria were plated on Columbia agar (Difco, Lublin, Poland) with 7% hemolysed horse blood (CA+HB) and incubated for 3 days under microaerophilic conditions (Genbox microaer kits, BioMerieux, Marcy I’Etoile, France) at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

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In Vitro Activity of 3-Bromopyruvate, an Anticancer Compound, Against Antibiotic-Susceptible and Antibiotic-Resistant Helicobacter pylori Strains

Krzyżek

Franiczek

Krzyżanowska

et al. 2019

Cancers

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Helicobacter pylori (H. pylori) is a bacterium capable of inducing chronic active gastritis, which in some people, develops into gastric cancers. One of the substances that may be useful in the eradication of this microorganism is 3-Bromopyruvate (3-BP), an anticancer compound with antimicrobial properties. The aim of this article was to determine the activity of 3-BP against antibiotic-susceptible and antibiotic-resistant H. pylori strains. The antimicrobial activity was determined using a disk-diffusion method, broth microdilution method, time-killing assay, and checkerboard assay. The research was extended by observations using light, fluorescence, and scanning electron microscopy. The growth inhibition zones produced by 2 mg/disk with 3-BP counted for 16–32.5 mm. The minimal inhibitory concentrations (MICs) ranged from 32 to 128 μg/mL, while the minimal bactericidal concentrations (MBCs) for all tested strains had values of 128 μg/mL. The time-killing assay demonstrated the concentration-dependent and time-dependent bactericidal activity of 3-BP. The decrease in culturability below the detection threshold (<100 CFU/mL) was demonstrated after 6 h, 4 h, and 2 h of incubation for MIC, 2× MIC, and 4× MIC, respectively. Bacteria treated with 3-BP had a several times reduced mean green/red fluorescence ratio compared to the control samples, suggesting bactericidal activity, which was independent from an induction of coccoid forms. The checkerboard assay showed the existence of a synergistic/additive interaction of 3-BP with amoxicillin, tetracycline, and clarithromycin. Based on the presented results, it is suggested that 3-BP may be an interesting anti-H. pylori compound.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

In Vitro Activity of 3-Bromopyruvate, an Anticancer Compound, Against Antibiotic-Susceptible and Antibiotic-Resistant Helicobacter pylori Strains

Krzyżek

Franiczek

Krzyżanowska

et al. 2019

Cancers

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“…Bacterial morphology was determined during the checkerboard method and time-killing assays [40]. Each time, 50 µL of bacterial suspension was dripped onto slides from each concentration and strain tested, and stained using the Gram's method.…”

Section: Light Microscopymentioning

confidence: 99%

“…Bacteria treated with certain concentrations of SER were fixed by adding a 2.5% glutaraldehyde solution (Sigma-Aldrich) [38,40]. After 24 h of fixing, bacteria were centrifuged at 600 g for 5 min and washed three times in 0.1 M cacodylate buffer (Sigma-Aldrich), centrifuging after each wash at 600 g for 5 min.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

“…As a result, Barry Marshall and Robin Warren were crowned with the Nobel Prize in Bacterial strains were stored in Tryptic Soy Broth (TSB) (Oxoid, Le Pont de Claix, France) with 15% glycerol at −70 • C until needed. After reviving, bacteria were seeded on Columbia agar (Difco, Lublin, Poland) with 7% hemolyzed horse blood (CA + HB) and incubated for three days under microaerophilic conditions (Genbox microaer kits, BioMerieux, Marcy I'Etoile, France) at 37 • C [38,40]. The grown bacteria were passaged on CA + HB and again incubated under previously mentioned conditions for the next three days.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Activity of Sertraline, an Antidepressant, Against Antibiotic-Susceptible and Antibiotic-Resistant Helicobacter pylori Strains

et al. 2019

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Antibiotic resistance of Helicobacter pylori, a spiral bacterium associated with gastric diseases, is a topic that has been intensively discussed in last decades. Recent discoveries indicate promising antimicrobial and antibiotic-potentiating properties of sertraline (SER), an antidepressant substance. The aim of the study, therefore, was to determine the antibacterial activity of SER in relation to antibiotic-sensitive and antibiotic-resistant H. pylori strains. The antimicrobial tests were performed using a diffusion-disk method, microdilution method, and time-killing assay. The interaction between SER and antibiotics (amoxicillin, clarithromycin, tetracycline, and metronidazole) was determined by using a checkerboard method. In addition, the study was expanded to include observations by light, fluorescence, and scanning electron microscopy. The growth inhibition zones were in the range of 19–37 mm for discs impregnated with 2 mg of SER. The minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) counted for 2–8 µg/mL and 4–8 µg/mL, respectively. The time-killing assay showed the time-dependent and concentration-dependent bactericidal activity of SER. Bacteria exposed to MBCs (but not sub-MICs and MICs ≠ MBCs) underwent morphological transformation into coccoid forms. This mechanism, however, was not protective because these cells after a 24-h incubation had a several-fold reduced green/red fluorescence ratio compared to the control. Using the checkerboard assay, a synergistic/additive interaction of SER with all four antibiotics tested was demonstrated. These results indicate that SER may be a promising anti-H. pylori compound.

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Reduction of Helicobacter pylori cells in rural water supply using slow sand filtration

Leyton,

Fernández,

Acosta

et al. 2024

Environ Monit Assess

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Helicobacter pylori is a microorganism that infects 60% of the population and is considered the main cause of atrophic gastritis, gastric and duodenal ulcers, and gastric cancer. Different emerging pathogens have been found in drinking water and their presence is considered to be an important public health problem. For this reason, it is necessary to carry out the validation of reliable technologies for this type of pathogens and evaluate their performance. This paper reports, for the first time, H. pylori reduction in a drinking water pilot plant of two slow sand filters (SSF). Inlet water was taken from a gravel filtration system of a rural water supply in Colombia and then inoculated with viable cells of H. pylori. By determining the Genomic Units (GU) through quantitative Polymerase Chain Reaction (qPCR), the concentration of GU/sample was measured. In the inlet water amplification for SSF1 and SSF2 were 5.13 × 102 ± 4.48 × 102 and 6.59 × 102 ± 7.32 × 102, respectively, while for the treated water they were 7.0 ± 5.6 and 2.05 × 101 ± 2.9 × 101 GU/sample for SSF1 and SSF2, respectively. The SSF pilot plant reached up to 3 log reduction units of H. pylori; therefore, since there is not an H. pylori contamination indicator and its periodic monitoring is financially complicated, the SSF could guarantee the drinking water quality necessity that exists in rural areas and small municipalities in developing countries, where infection rates and prevalence of this pathogen are high.

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Intensive formation of coccoid forms as a feature strongly associated with highly pathogenic Helicobacter pylori strains

Cited by 15 publications

References 45 publications

In Vitro Activity of 3-Bromopyruvate, an Anticancer Compound, Against Antibiotic-Susceptible and Antibiotic-Resistant Helicobacter pylori Strains

In Vitro Activity of 3-Bromopyruvate, an Anticancer Compound, Against Antibiotic-Susceptible and Antibiotic-Resistant Helicobacter pylori Strains

In Vitro Activity of Sertraline, an Antidepressant, Against Antibiotic-Susceptible and Antibiotic-Resistant Helicobacter pylori Strains

Reduction of Helicobacter pylori cells in rural water supply using slow sand filtration

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