Inter- and intra-individual variability of total antioxidant capacity of bovine seminal plasma and relationships with sperm quality before and after cryopreservation

Saini

Reprod Domestic Animals

et al. 2016

This study was attempted to identify subfertile bulls by quantifying the endogenous levels of osteopontin (OPN), total antioxidant capacity (TAC) and malondialdehyde (MDA) in seminal plasma of buffalo bulls. On the basis of conception rate, buffalo bulls were classified into two groups: high-fertile (conception rate >50%) and subfertile bulls (conception rate <40%). A total of 100 ejaculates (10 ejaculates from each bull) were collected through artificial vagina method. The concentration of OPN, TAC and catalase (CAT) of high-fertile bulls was found to be higher (p < .05) than that of subfertile bulls. Further, MDA level in seminal plasma was found to be lower (p < .05) in high-fertile bulls compared with subfertile bulls. The fertility status had no effect on the superoxide dismutase (SOD) concentration in seminal plasma of both the groups. The levels of OPN (r = .678, p = 0.013) and TAC (r = .648, p = .042) were found to be positively correlated with bull fertility and the level of MDA (r = -.718, p = .019) was found to be negatively correlated with bull fertility. However, the fertility of bulls was not found to be significantly correlated with SOD, CAT and sperm motility. In conclusion, seminal OPN, TAC and MDA tended to be more realistic in identification of subfertile bulls from breeding herds.

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo (Bubalus bubalis) bulls

Saini

Reprod Domestic Animals

et al. 2016

“…Briefly, sperm motility was determined by CASA according to the methods used on fresh ejaculates, and all other sperm parameters were analysed using flow cytometry (Cell Lab Quanta SC MPL, Beckman Couler Inc., Nyon, Switzerland). Plasma membrane and acrosome integrity of spermatozoa defined as viability were measured after staining with propidium iodide (PI) and peanut agglutinin conjugated with fluorescein isothiocyanate (FITC‐PNA) (Wach‐Gygax et al., ), and the amount of lipid peroxidation of plasma membrane intact sperm was determined after incubation with 4,4‐difluoro‐5‐(4‐phenyl‐1,3‐butadienyl)‐4‐bora‐3a,4a‐diaza‐s‐indacene‐3‐undecanoic acid (BODIPY) and PI (Gürler, Calisici, & Bollwein, ). The percentages of plasma membrane intact sperm with a high mitochondrial membrane potential (HMMP) or a low intracellular Ca 2+ concentration were determined, combining PI with JC‐1 and Fluo‐4, respectively (Malama et al., ).…”

Section: Methodsmentioning

confidence: 99%

Stallion semen quality depends on major histocompatibility complex matching to teaser mare

et al. 2018

The major histocompatibility complex (MHC) has repeatedly been found to influence mate choice of vertebrates, with MHC-dissimilar mates typically being preferred over MHC-similar mates. We used horses (Equus caballus) to test whether MHC matching also affects male investment into ejaculates after short exposure to a female. Semen characteristics varied much among stallions. Controlling for this variance with a full-factorial within-subject experimental design, we found that a short exposure to an MHC-dissimilar mare enhanced male plasma testosterone and led to ejaculates with elevated sperm numbers as compared to exposure to an MHC-similar mare. Sperm velocity seemed not affected by the treatment. Overall genetic similarity between stallions and mares (determined from polymorphic microsatellites on 20 different chromosomes) played no significant role here. The MHC type of the teaser mare also affected characteristics of cold-stored sperm after 24 and 48 hr. As expected from ejaculate economics, sperm viability was elevated after exposure to an MHC-dissimilar mare. However, oxidative stress and the percentage of sperm with a high DNA fragmentation were mostly increased after exposure to an MHC-dissimilar mare, depending also on whether the teaser mare was in oestrous or not. We conclude that males can quickly adjust ejaculate quality relative to a female's MHC, and that this male reaction to the social environment can also affect important characteristics of cold-stored semen.

Rev. investig. vet. Perú

“…Algunos de estos suplementos podrían tener efecto sobre la actividad antioxidante del diluyente, como es el caso de los fosfatos, cuyas propiedades antioxidantes se cree están basadas en su acción como quelantes (Weilmeier y Regenstein, 2004). La quercetina es uno de los antioxidantes con efectos favorables sobre el semen equino congelado, pues mejora la movilidad y la habilidad de unión a la zona pelúcida, evita la capacitación prematura y reduce la peroxidación lipídica y la fragmentación del A pesar de lo anterior, pocas veces se ha considerado el aporte de los antioxidantes y otros suplementos a la capacidad antioxidante total (CAT) del diluyente; no obstante, su importancia ha sido ampliamente demostrada en la criotolerancia del semen de especies como carneros (Yildiz et al, 2015), porcinos (Barranco et al, 2015) y bovinos (Gürler et al, 2015). En equinos, estudios previos han relacionado la CAT del plasma seminal con diferentes parámetros de calidad espermática (Wnuk et al, 2010;Restrepo et al, 2015).…”

Section: Introductionunclassified

Criotolerancia de Semen Equino Congelado con Aditivos en el Diluyente

Duque

Rojano

Betancur

2017

RESUMENLos diluyentes se han constituido como una alternativa para compensar las deficiencias antioxidantes del semen equino durante su congelación. Muchas investigaciones han estado dirigidas a evaluar el efecto de diversos aditivos con propiedades antioxidantes sobre la criotolerancia de los espermatozoides equinos; sin embargo, en limitadas ocasiones se ha relacionado dicha propiedad con la capacidad antioxidante total (CAT) del diluyente. Esta investigación tuvo como objetivo evaluar el efecto de dos aditivos sobre la CAT del diluyente de congelación y la criotolerancia del semen equino. El semen de cinco caballos criollos colombianos se congeló con un diluyente en tres tratamientos: T0: control, T1: quercetina 100 µM, T2: fosfatos y carbonatos (Na 2 HPO 4 0.32 mg/ml + KH 2 PO 4 0.67 mg/ml + K 2 CO 3 0.39 mg/ml). Se evaluó la CAT del diluyente mediante la prueba ABTS. En el semen descongelado se evaluó la movilidad y la cinética, la morfología anormal (MA) y la integridad estructural (IEM) y funcional (HOS) de la membrana plasmática de los espermatozoides. Los resultados se analizaron mediante modelos mixtos. Se hallaron valores de CAT (µmol trolox/l) para T0, T1 y T2 de 10890 ± 210, 14260 ± 410 y 14670 ± 410, respectivamente. El T2 presentó valores superiores para la mayoría de parámetros de movilidad e integridad de membrana (p<0.05). Se concluye que la adición de fosfatos y carbonatos incrementa la CAT del diluyente de congelación y mejora la criotolerancia del semen equino.Palabras clave: diluyente, criopreservación, carbonatos, fosfatos, quercetina ABSTRACT Extenders have been established as an alternative to compensate the antioxidant deficiencies of stallion semen during freezing. Several research works have aimed to evaluate the effect of various additives with antioxidant properties on the equine sperm