Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency

Ainsbury, Elizabeth A.; Al-hafidh, Jenna; Bajinskis, Ainars; Barnard, Stephen; Barquinero, Joan Francesc; Beinke, Christina; Gelder, Virginie de; Grégoire, Éric; Jaworska, A; Lindholm, Carita; Lloyd, David; Moquet, Jayne; Nylund, R; Oestreicher, Ursula; Roch-Lefèvre, Sandrine; Rothkamm, Kai; Romm, Horst; Scherthan, Harry; Sommer, Sylwester; Thierens, Hubert; Vandevoorde, Charlot; Vral, Anne; Wójcik, Andrzej

doi:10.3109/09553002.2014.868616

Cited by 46 publications

(34 citation statements)

References 12 publications

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“…When a new dosimetry technique is proposed, it is necessary to develop, test and then validate the method before it can be said to reach a sufficient standard for inclusion as a fully operational dosimetry method. Inter-comparison exercises are a recognised method for validating tools and techniques within the field of biological and physical retrospective dosimetry (Badie et al 2013;Romm et al 2013;Ainsbury et al 2014;Abend et al 2016).…”

Section: Integration Of New Biological and Physical Retrospective Dosmentioning

confidence: 99%

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Ainsbury

Badie

Barnard

et al. 2016

International Journal of Radiation Biology

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Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There is a requirement to test and/or validate these candidate techniques and inter-comparison exercises are a well-established method for such validation. Materials and methods: The authors present details of inter-comparisons of four such new methods: dicentric chromosome analysis including telomere and centromere staining; the gene expression assay carried out in whole blood; Raman spectroscopy on blood lymphocytes, and detection of radiationinduced thermoluminescent signals in glass screens taken from mobile phones. Results: In general the results show good agreement between the laboratories and methods within the expected levels of uncertainty, and thus demonstrate that there is a lot of potential for each of the candidate techniques. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2017 VOL. 93, NO. 1, 99-109 http://dx.doi.org/10.1080/09553002.2016 Conclusions: Further work is required before the new methods can be included within the suite of reliable dosimetry methods for use by RENEB partners and others in routine and emergency response scenarios.

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Section: Integration Of New Biological and Physical Retrospective Dosmentioning

confidence: 99%

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Ainsbury

Badie

Barnard

et al. 2016

International Journal of Radiation Biology

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“…Timed intercomparisons have been described by Rothkamm et al (2013a) and Ainsbury et al (2014) with triage dose estimates, based on foci counts in 50 cells, reported within 7 h (10 samples) and 3 h (8 samples), respectively. With a more rapid processing technique and scoring just 20 cells it may be possible to produce dose estimates for 96 samples in 7-11 h (Moquet et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…With a more rapid processing technique and scoring just 20 cells it may be possible to produce dose estimates for 96 samples in 7-11 h (Moquet et al 2014). Past experience (Rothkamm et al 2013a;Ainsbury et al 2014) and the first inter-comparison have shown that for rapid triage purposes, i.e. detection of moderate to high doses, manual scoring of 50 cells or 200 cells scored automatically, is fully sufficient and even scoring 20 cells manually or 50 automatically, may be sufficiently accurate in a triage setting.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown that rapid processing time of the gamma-H2AX assay can produce dose estimates within a few hours of receiving a blood sample (Rothkamm et al 2013a;Ainsbury et al 2014). In addition, the potential for batch processing on finger prick blood samples, enabling high throughput (Moquet et al 2014), makes the assay ideal for early triage categorization to separate the 'worried well' from critically exposed individuals by quantifying radiation-induced foci in peripheral blood lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

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The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)

Moquet

Barnard

Staynova

et al. 2016

International Journal of Radiation Biology

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Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples. Results: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty. Conclusions: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories. ARTICLE HISTORY

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“…In such cases, it may be imperative to screen tens or hundreds of thousands of individuals for radiation exposure, both to identify and prioritize individuals that would benefit from medical treatment and to alleviate the concerns of the “worried well” (3–5). The threat of large-scale radiological incidents has led to a number of recent developments in the fields of biological and physical dosimetry (6). However, to triage potentially large numbers of people exposed to ionizing radiation, the development of high-throughput approaches for radiation biodosimetry has been identified as a high priority.…”

Section: Introductionmentioning

confidence: 99%

Development of a High-Throughput and Miniaturized Cytokinesis-Block Micronucleus Assay for Use as a Biological Dosimetry Population Triage Tool

et al. 2015

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Biodosimetry is an essential tool for providing timely assessments of radiation exposure. For a large mass-casualty event involving exposure to ionizing radiation, it is of utmost importance to rapidly provide dose information for medical treatment. The well-established cytokinesis-block micronucleus (CBMN) assay is a validated method for biodosimetry. However, the need for an accelerated sample processing is required for the CBMN assay to be a suitable population triage tool. We report here on the development of a high-throughput and miniaturized version of the CMBN assay for accelerated sample processing.

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Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency

Abstract: The data demonstrate that the MULTIBIODOSE approach of applying multiparametric tools to radiation emergencies is valid and effective.

Cited by 46 publications

References 12 publications

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)

Development of a High-Throughput and Miniaturized Cytokinesis-Block Micronucleus Assay for Use as a Biological Dosimetry Population Triage Tool

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