Inter- and Intra-Varietal Genetic Variability in Malvasia Cultivars

Meneghetti, Stefano; Poljuha, Danijela; Frare, E.; Costacurta, Angelo; Morreale, Giacomo; Bavaresco, Luigi; Calò, Antonio

doi:10.1007/s12033-011-9423-5

Cited by 15 publications

(11 citation statements)

References 32 publications

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“…Many studies have reported the intra-varietal genetic variability [41–43]. However, only the genotypic variations among cultivated bromeliads were investigated, with the genetic variability within asexual clones of genotypes neglected in this study.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Cultivated Bromeliad Accessions with Inter-Simple Sequence Repeat (ISSR) Markers

Zhang

Wang

et al. 2012

IJMS

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Bromeliads are of great economic importance in flower production; however little information is available with respect to genetic characterization of cultivated bromeliads thus far. In the present study, a selection of cultivated bromeliads was characterized via inter-simple sequence repeat (ISSR) markers with an emphasis on genetic diversity and population structure. Twelve ISSR primers produced 342 bands, of which 287 (~84%) were polymorphic, with polymorphic bands per primer ranging from 17 to 34. The Jaccard’s similarity ranged from 0.08 to 0.89 and averaged ~0.30 for the investigated bromeliads. The Bayesian-based approach, together with the un-weighted paired group method with arithmetic average (UPGMA)-based clustering and the principal coordinate analysis (PCoA), distinctly grouped the bromeliads from Neoregelia, Guzmania, and Vriesea into three separately clusters, well corresponding with their botanical classifications; whereas the bromeliads of Aechmea other than the recently selected hybrids were not well assigned to a cluster. Additionally, ISSR marker was proven efficient for the identification of hybrids and bud sports of cultivated bromeliads. The findings achieved herein will further our knowledge about the genetic variability within cultivated bromeliads and therefore facilitate breeding for new varieties of cultivated bromeliads in future as well.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Cultivated Bromeliad Accessions with Inter-Simple Sequence Repeat (ISSR) Markers

Zhang

Wang

et al. 2012

IJMS

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“…Minor genetic variation was observed among the different "Rocha" clones, which could be due to somatic mutations related to grafting [35] or, in some cases, the results of hybridization with unknown pear plants. Previously, a great variability and heterogeneity was referred among Malvasia grapevine clones that were cultivated in different geographic areas [36,37]. Clonal variation has also been reported within ancient Portuguese grapevine varieties [38].…”

Section: Genetic Relationships Among Pear Genotypesmentioning

confidence: 93%

Genetic Diversity and Structure of the Portuguese Pear (Pyrus communis L.) Germplasm

et al. 2019

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A rich heritage of traditional pear varieties is kept in national Portuguese collections. Out of these varieties, "Rocha" dominates national pear production. Although a noticeable phenotypic variation among clones of this variety has been reported, little is known about its genetic variability, as to date molecular studies have been performed on a single "Rocha" clone. Eleven Simple Sequence Repeats (SSR) markers were used to assess the genetic diversity of 130 local cultivars, 80 of them being "Rocha" clones. The results allowed the differentiation of 75 genotypes of which 29 are "Rocha". Three synonyms groups and four homonymous groups of other local varieties were confirmed. A Bayesian model-based clustering approach identified two distinct clusters. Using flow cytometry, six cultivars were found to be triploids. These results show high genetic variability among "Rocha" clones. In conclusion, there is a need for different "Rocha" clones to be preserved to enable the correct selection of the multiplication material.

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“…Amplified fragment length polymorphisms analysis has proven useful for discriminating among grapevine cultivars [46] but the method has the disadvantage of generating a complex series of bands that may be difficult to interpret and standardize. However, this method and modified versions have shown to be powerful to investigate polymorphisms among clones and assess inter-and intracultivar diversity [47][48][49][50].…”

Section: Dna-based Methodsmentioning

confidence: 99%

“…A set of six specific dinucleotide STRs already widely used (VVMD5, VVMD7, VVDM27, VVS2, VrZAG62, and VrZAG79) was agreed upon by the members for future analyses (Table 1). After converting raw data into normalized data through a coding method, the authors reported discrepancies of the results among the members: genotyping errors, [17,20,23,24,28,33,35,41,49,50,65,[69][70][71][72][74][75][76][77][78][79][80][81][82][83][84][85][86]89,91,92] SsrVrZAG79 [17,20,23,24,28,33,35,41,49,50,65,[69][70][71][72][74][75]…”

Section: Commonly Used Strs For Grapevine Genotypingmentioning

confidence: 99%

A forensic perspective on the genetic identification of grapevine (Vitis vinifera L.) varieties using STR markers

et al. 2014

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The grapevine (Vitis vinifera subsp. vinifera) is one of the most important agricultural crops worldwide. A long interest in the historical origins of ancient and cultivated current grapevines, as well as the need to establish phylogenetic relationships and parentage, solve homonymies and synonymies, fingerprint cultivars and clones, and assess the authenticity of plants and wines has encouraged the development of genetic identification methods. STR analysis is currently the most commonly used method for these purposes. A large dataset of grapevines genotypes for many cultivars worldwide has been produced in the last decade using a common set of recommended dinucleotide nuclear STRs. This type of marker has been replaced by long core-repeat loci in standardized state-of-the-art human forensic genotyping. The first steps toward harmonized grapevine genotyping have already been taken to bring the genetic identification methods closer to human forensic STR standards by previous authors. In this context, we bring forward a set of basic suggestions that reinforce the need to (i) guarantee trueness-to-type of the sample; (ii) use the long core-repeat markers; (iii) verify the specificity and amplification consistency of PCR primers; (iv) sequence frequent alleles and use these standardized allele ladders; (v) consider mutation rates when evaluating results of STR-based parentage and pedigree analysis; (vi) genotype large and representative samples in order to obtain allele frequency databases; (vii) standardize genotype data by establishing allele nomenclature based on repeat number to facilitate information exchange and data compilation.

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Inter- and Intra-Varietal Genetic Variability in Malvasia Cultivars

Cited by 15 publications

References 32 publications

Molecular Characterization of Cultivated Bromeliad Accessions with Inter-Simple Sequence Repeat (ISSR) Markers

Molecular Characterization of Cultivated Bromeliad Accessions with Inter-Simple Sequence Repeat (ISSR) Markers

Genetic Diversity and Structure of the Portuguese Pear (Pyrus communis L.) Germplasm

A forensic perspective on the genetic identification of grapevine (Vitis vinifera L.) varieties using STR markers

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