Inter- and intraspecific genetic variation inHippophae (Elaeagnaceae) investigated by RAPD markers

Bartish, Igor V.; Jeppsson, N.; Bartish, Galyna I; Lu, Rong; Nybom, Hilde

doi:10.1007/bf00985460

Cited by 59 publications

(69 citation statements)

References 20 publications

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“…He. This hypothesis was subsequently confirmed by analysis of random amplification of polymorphic DNA (RAPD) markers, internal transcribed spacer (ITS) sequence data and morphological data (Bartish et al, 2000(Bartish et al, , 2002Sun et al, 2003). In addition, H. goniocarpa was found to have double maternal origin from both putative parental species, and this homoploid hybrid species is probably in an early stage of speciation following initial reproductive isolation (Wang et al, 2008); some ecophysiological traits superior to both parents have been established in this species (Ma et al 2009).…”

Section: Introductionmentioning

confidence: 75%

“…Besides these three species, several shrub or tree species have been described under the genus Hippophae (sea buckthorns) (Rousi, 1971;Lian et al, 1996;Bartish et al, 2002), all of which have received a great deal of attention as important nitrogen-fixing species for afforestation (Lian et al 1996, Tian et al 2004 or their fruits as raw materials in soft-drink production (Lian et al 1996). These species are wind-pollinated and dioecious, with gender determined genetically (Rousi, 1971;Lian et al, 1998;Bartish et al, 2000). So far all of them have been shown to be diploid with 2n = 24 (Rousi, 1971;Lian et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Genome Size of the Diploid Hybrid Species Hippophae goniocarpa and Its Parental Species, H. rhamnoides ssp. sinensis and H. neurocarpa ssp. neurocarpa (Elaeagnaceae)

Zhou¹,

Ma²,

Wang³

et al. 2010

Acta Biologica Cracoviensia Series Botanica

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Hybridization may induce rapid genomic changes, including gain or loss of DNA. We examined nuclear DNA content of a diploid hybrid species, Hippophae goniocarpa and its two parental species. The nuclear 2C DNA value was 2.95±0.08 pg for H. goniocarpa; for the parental species it ranged from 2.54±0.09 pg to 2.66±0.12 pg for H. rhamnoides ssp. sinensis and from 3.15±0.19 pg to 3.49±0.06 pg for H. neurocarpa ssp. neurocarpa. The nuclear DNA content of H. goniocarpa was intermediate between those of the two parental species, confirming that this species is of diploid hybrid origin and further suggesting that it is in an early stage of the process of speciation.K Ke ey y w wo or rd ds s: : Nuclear DNA content, flow cytometry, H. goniocarpa, hybrid species, interspecific and intraspecific variation.

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Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Genome Size of the Diploid Hybrid Species Hippophae goniocarpa and Its Parental Species, H. rhamnoides ssp. sinensis and H. neurocarpa ssp. neurocarpa (Elaeagnaceae)

Zhou¹,

Ma²,

Wang³

et al. 2010

Acta Biologica Cracoviensia Series Botanica

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“…In several studies, lower G ST (between population diversity) values were found for allozymes than Random Amplified Polymorphic DNAs (RAPDs) (Ayres and Ryan, 1997;Latta and Mitton, 1997;Comes and Abbott, 2000), although other studies detected no difference (Isabel et al, 1995;Szmidt et al, 1996;Le Corre et al, 1997;Aagaard et al, 1998) or lower population differentiation in RAPDs has occasionally been detected (Allendorf and Seeb, 2000;Bartish et al, 2000b). As with quantitative vs DNA/allozyme markers, discordant patterns of divergence in several molecular markers and loci are commonly interpreted as the differential effect of natural selection on those markers and loci (Karl and Avise, 1992;McDonald, 1994;Latta and Mitton, 1997), despite no direct evidence of the latter.…”

Section: Introductionmentioning

confidence: 99%

Distinguishing adaptive from nonadaptive genetic differentiation: comparison of QST and FST at two spatial scales

et al. 2005

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Genetic differentiation in 20 hierarchically sampled populations of wild barley was analyzed with quantitative traits, allozymes and Random Amplified Polymorphic DNAs (RAPDs), and compared for three marker types at two hierarchical levels. Regional subdivision for both molecular markers was much lower than for quantitative traits. For both allozymes and RAPDs, most loci exhibited minor or no regional differentiation, and the relatively high overall estimates of the latter were due to several loci with exceptionally high regional differentiation. The allozymeand RAPD-specific patterns of differentiation were concordant in general with one another, but not with quantitative trait differentiation. Divergent selection on quantitative traits inferred from very high regional Q ST was in full agreement with our previous results obtained from a test of local adaptation and multilevel selection analysis. In contrast, most variation in allozyme and RAPD variation was neutral, although several allozyme loci and RAPD markers were exceptional in their levels of regional differentiation. However, it is not possible to answer the question whether these exceptional loci are directly involved in the response to selection pressure or merely linked to the selected loci. The fact that Q ST and F ST did not differ at the population scale, that is, within regions, but differed at the regional scale, for which local adaptation has been previously shown, implies that comparison of the level of subdivision in quantitative traits, as compared with molecular markers, is indicative of adaptive population differentiation only when sampling is carried out at the appropriate scale. Heredity (2005) 95, 466-475.

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“…Тем не менее в работах ряда зарубеж-ных авторов предприняты попытки расшифровки генома этой культуры, относящиеся, однако, исключительно к крупным систематическим группам -видам и подвидам. Наиболее часто встречаемые молекулярные методы в исследовании генома облепихи -это RAPD, AFLP, ISSR, SSR (Yao et al,1993;Bartish et al, 1999Bartish et al, , 2000Bartish et al, , 2003Sun et al, 2002;Yongshan et al, 2003a, b;Tian et al, 2004;Сhen et al, 2008;Li et al, 2009;Wang et al, 2011;Raina et al, 2012;Jain et al, 2014).…”

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Assessment of genetic polymorphism in sea buckthorn (Hippophae L.) of different environmentally-geographical origin by ISSR-markers

Земцова¹,

Зубарев²,

Gunin³

et al. 2017

Vestn. VOGiS

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Sea buckthorn, despite of high popularity at the moment, is not a common object of genetic researches. Some efforts of foreign scientists in this field are concerned with only big systematic groups like species and subspecies. At the same time genetic researches inside of subspecies mongolica growing in Siberia till recently have not been started. There are too many so called ecotypes of sea buckthorn belonging to Hippo phae rhamnoides ssp. mongolica growing in different environmentally-geographical areas. That is why the task of deep genetic research of that subspecies is very important. The absence of an approved procedure of genome definition for Siberian sea buckthorn makes the above task quite complicated. That is why the main aim of the current investigation was developing a procedure of ISSR-analysis for sea buckthorn growing in Siberia, as well as preliminary estimation of genetic polymorphism of sea buckthorn varieties belonging to different environmentally-geographical areas by methods of molecular markers. As a result, the procedure of ISSR-analysis for sea buckthorn growing in Siberia has been developed. From 32 estimated ISSR-markers only 6 have been educed as effective for ISSR-locus polymorphism evaluation of sea buckthorn. The annealing temperature for each primer has been found. Analyses of ISSR-markers polymorphism as well as genetic diversity of 17 sea buckthorn varieties have been done. ISSR-spectrum analyses of sea buckthorn plants have educed 56 amplified DNA fragments, 36 of them were polymorphic. According to obtained data a dendrogram of genetic affinity of evaluated varieties has been built, where one variety generates one cluster. In general, it conforms to the idea that one species is divided into subspecies and one subspecies is divided into different ecotypes. As a result of cluster Облепиха, несмотря на всю ее популярность, не является массо-вым объектом генетических исследований. Предпринятые рядом зарубежных авторов попытки генотипирования этой культуры относятся исключительно к крупным систематическим группам -видам и подвидам. Вместе с тем чрезвычайно актуальным пред-ставляется изучение внутривидового генетического разнообразия облепихи Hippophae rhamnoides ssp. mongolica, произрастающей в Сибири, что обусловлено наличием большого количества так на-зываемых экотипов, приуроченных к различным эколого-геогра-фическим провинциям. Отсутствие проверенной и отработанной методики определения структуры генома сибирской облепихи усложняет обозначенную задачу. В этой связи целью настоящего исследования была отработка методики ISSR-анализа примени-тельно к образцам облепихи, произрастающим в условиях Сибири, а также предварительная оценка генетического полиморфизма сортообразцов облепихи различной популяционно-систематиче-ской принадлежности посредством методов молекулярного мар-кирования. В результате проведенных исследований отрабо тана методика ISSR-анализа для облепихи, произрастающей в Си бири. Из 32 протестированных ISSR-маркеров выявлены шесть эффек-тивных для анализа полиморфизма ...

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Inter- and intraspecific genetic variation inHippophae (Elaeagnaceae) investigated by RAPD markers

Cited by 59 publications

References 20 publications

Genome Size of the Diploid Hybrid Species Hippophae goniocarpa and Its Parental Species, H. rhamnoides ssp. sinensis and H. neurocarpa ssp. neurocarpa (Elaeagnaceae)

Genome Size of the Diploid Hybrid Species Hippophae goniocarpa and Its Parental Species, H. rhamnoides ssp. sinensis and H. neurocarpa ssp. neurocarpa (Elaeagnaceae)

Distinguishing adaptive from nonadaptive genetic differentiation: comparison of QST and FST at two spatial scales

Assessment of genetic polymorphism in sea buckthorn (Hippophae L.) of different environmentally-geographical origin by ISSR-markers

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