Inter-laboratory skin distribution study of 4-n-butyl resorcinol: The importance of liquid chromatography/mass spectrometry (HPLC–MS/MS) bioanalytical validation

Wargniez, William; Jungman, Elsa; Wilkinson, Simon C.; Seyler, Nathalie; Grégoire, Sébastien

doi:10.1016/j.jchromb.2017.05.026

Cited by 11 publications

(5 citation statements)

References 9 publications

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“…The dedicated analytical method was validated for each chemical (e.g. carvacrol and ceramide), according to previously defined criteria 48 . These criteria are classically used for validation of bioanalytical method 49 .…”

Section: Methodsmentioning

confidence: 99%

Imaging the distribution of skin lipids and topically applied compounds in human skin using mass spectrometry

Sjövall

Skedung

Grégoire

et al. 2018

Sci Rep

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The barrier functions of skin against water loss, microbial invasion and penetration of xenobiotics rely, in part, on the spatial distribution of the biomolecular constituents in the skin structure, particularly its horny layer (stratum corneum). However, all skin layers are important to describe normal and dysfunctional skin conditions, and to develop adapted therapies or skin care products. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) combined with scanning electron microscopy (SEM) was used to image the spatial distribution of a variety of molecular species, from stratum corneum down to dermis, in cross-section samples of human abdominal skin. The results demonstrate the expected localization of ceramide and saturated long-chain fatty acids in stratum corneum (SC) and cholesterol sulfate in the upper part of the viable epidermis. The localization of exogenous compounds is demonstrated by the detection and imaging of carvacrol (a constituent of oregano or thyme essential oil) and ceramide, after topical application onto ex vivo human skin. Carvacrol showed pronounced accumulation to triglyceride-containing structures in the deeper parts of dermis. In contrast, the exogenous ceramide was found to be localized in SC. Furthermore, the complementary character of this approach with classical ex vivo skin absorption analysis methods is demonstrated.

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Section: Methodsmentioning

confidence: 99%

Imaging the distribution of skin lipids and topically applied compounds in human skin using mass spectrometry

Sjövall

Skedung

Grégoire

et al. 2018

Sci Rep

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“…Clinical use of RSV as a depigmenting agent has been limited yet, but topical resveratryl triacetate—a prodrug of RSV—and resveratryl triglycolate—a hybrid compound of RSV and glycolic acid—were shown to exhibit a significant reduction in UV‐induced pigmentation . Topically applied 4nBR or RSV was reported to penetrate into the skin …”

Section: Discussionmentioning

confidence: 99%

“…9,10 Topically applied 4nBR or RSV was reported to penetrate into the skin. 19,20 We previously demonstrated that combination of 4nBR and RSV at a low dose synergistically reduced melanin synthesis in vitro. 11 Though 1μM of 4nBR and 1μM of RSV did not individually inhibit melanin synthesis, the combination of 4nBR and RSV at the same concentration significantly reduced melanin synthesis by lowering tyrosinase levels.…”

Section: Rsv Inhibits Melanogenesis Through Various Mechanisms Rsvmentioning

confidence: 99%

Efficacy of liposome‐encapsulated 4‐n‐butylresorcinol and resveratrol cream in the treatment of melasma

Kwon

Yang

Shin

et al. 2019

J of Cosmetic Dermatology

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Backgrounds We previously reported the efficacy of 0.1% 4‐n‐butylresorcinol (4nBR) cream in the treatment melasma and synergistic effect of 4nBR and resveratrol (RSV) to inhibit melanogenesis in vitro. Aims To evaluate efficacy and safety of a cream which contains liposome‐encapsulated 4nBR and RSV in the treatment of melasma. Patients/Methods A total of 21 female patients with melasma were treated with the cream for 4 weeks. At baseline, week 2, and week 4, melanin index (MI) of the lesional and preauricular nonlesional skin was measured and two blinded, independent dermatologists assessed the overall severity by 5‐point scale. Results The lesional MI was significantly decreased at weeks 2 and 4 compared with the baseline while no significant change in the nonlesional MI was observed throughout the study. The mean investigator's global assessment score was also significantly improved at weeks 2 and 4. In patient's self‐assessment, 8 (38.1%) and 11 (52.3%) patients answered moderate to significant improvement in their melasma at weeks 2 and 4, respectively. No serious adverse events were reported. Conclusion The cream containing liposome‐encapsulated 4nBR and RSV was shown to be effective and safe for the treatment of melasma with its effect appearing as early as 2 weeks.

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“…Others agents include azelaic acid, kojic acid, retinoid treatments, niacinamide, corticosteroid medications, salicylic and glycolic acid, arbutin, resveratrol, and resorcinol. These agents and their mechanisms are cited in Table 1 (Al-Niaimi and Chiang, 2017, Birk, 1985, Bissett, 2002, De Caprio, 1999, Deo et al, 2013, Glowka et al, 2018, Grimes, 1995, Grimes, 2009, Hashim et al, 2018; Huh et al, 2010; Kang, 2005; Keeling et al, 2008; Lajis et al, 2012, Menter, 2004, Monteiro et al, 2013, Navarrete-Solís et al, 2011, Niwano et al, 2018, Nordlund et al, 2006, Olejnik et al, 2018, Paine et al, 2001, Picardo and Carrera, 2007, Pires et al, 2018, Schulte et al, 2015, Tse, 2010; Videira et al, 2013; Wargniez et al, 2017, Wohlrab and Kreft, 2014). Evidence-based studies have suggested that combination products that contain hydroquinone 4%, tretinoin, and a steroid produce the best response (Jutley et al, 2014, Rivas and Pandya, 2013; Sarkar, 2013; Sarma et al, 2017).…”

Section: Frequently Used Topical Agentsmentioning

confidence: 99%

New oral and topical approaches for the treatment of melasma

Grimes

Ijaz

Nashawati

et al. 2019

International Journal of Women's Dermatology

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Melasma is a common, therapeutically challenging, and universally relapsing disorder of hyperpigmentation that is most often observed in women and individuals with Fitzpatrick Skin Types III through VI. The pathogenesis of melasma is complex and protean. Contributing factors that are often implicated in the etiopathogenesis of this condition include a genetic predisposition, intense ultraviolet radiation exposure, and hormonal influences. Therapeutic interventions for melasma include a multimodality approach incorporating photoprotection agents, topical and oral skin lighteners, and resurfacing procedures. Given our expanding knowledge of the pathogenesis of melasma, new and effective treatments are expanding our therapeutic armamentarium. This article reviews new and emerging oral and topical treatments for melasma.

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Inter-laboratory skin distribution study of 4-n-butyl resorcinol: The importance of liquid chromatography/mass spectrometry (HPLC–MS/MS) bioanalytical validation

Cited by 11 publications

References 9 publications

Imaging the distribution of skin lipids and topically applied compounds in human skin using mass spectrometry

Imaging the distribution of skin lipids and topically applied compounds in human skin using mass spectrometry

Efficacy of liposome‐encapsulated 4‐n‐butylresorcinol and resveratrol cream in the treatment of melasma

New oral and topical approaches for the treatment of melasma

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